Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease
Abstract The coronavirus disease-19 (COVID-19) pandemic has already claimed millions of lives and remains one of the major catastrophes in the recorded history. While mitigation and control strategies provide short term solutions, vaccines play critical roles in long term control of the disease. Rec...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13560-z |
| _version_ | 1818256970411409408 |
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| author | Manolo Fernández Díaz Katherine Calderón Aldo Rojas-Neyra Vikram N. Vakharia Ricardo Choque-Guevara Angela Montalvan-Avalos Astrid Poma-Acevedo Dora Rios-Matos Andres Agurto-Arteaga Maria de Grecia Cauti-Mendoza Norma Perez-Martinez Gisela Isasi-Rivas Luis Tataje-Lavanda Yacory Sernaque-Aguilar Freddy Ygnacio Manuel Criollo-Orozco Edison Huaccachi-Gonzalez Elmer Delgado-Ccancce Doris Villanueva-Pérez Ricardo Montesinos-Millán Kristel Gutiérrez-Manchay Katherinne Pauyac-Antezana Ingrid Ramirez-Ortiz Stefany Quiñones-Garcia Yudith Cauna-Orocollo Katherine Vallejos-Sánchez Angela Rios-Angulo Dennis Núñez-Fernández Mario I. Salguedo-Bohorquez Julio Ticona Manolo Fernández-Sánchez Eliana Icochea Luis A. Guevara-Sarmiento Mirko Zimic COVID-19 Working Group in Perú |
| author_facet | Manolo Fernández Díaz Katherine Calderón Aldo Rojas-Neyra Vikram N. Vakharia Ricardo Choque-Guevara Angela Montalvan-Avalos Astrid Poma-Acevedo Dora Rios-Matos Andres Agurto-Arteaga Maria de Grecia Cauti-Mendoza Norma Perez-Martinez Gisela Isasi-Rivas Luis Tataje-Lavanda Yacory Sernaque-Aguilar Freddy Ygnacio Manuel Criollo-Orozco Edison Huaccachi-Gonzalez Elmer Delgado-Ccancce Doris Villanueva-Pérez Ricardo Montesinos-Millán Kristel Gutiérrez-Manchay Katherinne Pauyac-Antezana Ingrid Ramirez-Ortiz Stefany Quiñones-Garcia Yudith Cauna-Orocollo Katherine Vallejos-Sánchez Angela Rios-Angulo Dennis Núñez-Fernández Mario I. Salguedo-Bohorquez Julio Ticona Manolo Fernández-Sánchez Eliana Icochea Luis A. Guevara-Sarmiento Mirko Zimic COVID-19 Working Group in Perú |
| author_sort | Manolo Fernández Díaz |
| collection | DOAJ |
| description | Abstract The coronavirus disease-19 (COVID-19) pandemic has already claimed millions of lives and remains one of the major catastrophes in the recorded history. While mitigation and control strategies provide short term solutions, vaccines play critical roles in long term control of the disease. Recent emergence of potentially vaccine-resistant and novel variants necessitated testing and deployment of novel technologies that are safe, effective, stable, easy to administer, and inexpensive to produce. Here we developed three recombinant Newcastle disease virus (rNDV) vectored vaccines and assessed their immunogenicity, safety, and protective efficacy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in mice and hamsters. Intranasal administration of rNDV-based vaccine candidates elicited high levels of neutralizing antibodies. Importantly, the nasally administrated vaccine prevented lung damage, and significantly reduced viral load in the respiratory tract of vaccinated animal which was compounded by profound humoral immune responses. Taken together, the presented NDV-based vaccine candidates fully protected animals against SARS-CoV-2 challenge and warrants evaluation in a Phase I human clinical trial as a promising tool in the fight against COVID-19. |
| first_indexed | 2024-12-12T17:36:13Z |
| format | Article |
| id | doaj.art-696e9de1515149608bd5186f685f0c3b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T17:36:13Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-696e9de1515149608bd5186f685f0c3b2022-12-22T00:17:12ZengNature PortfolioScientific Reports2045-23222022-06-0112111810.1038/s41598-022-13560-zIntranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 diseaseManolo Fernández Díaz0Katherine Calderón1Aldo Rojas-Neyra2Vikram N. Vakharia3Ricardo Choque-Guevara4Angela Montalvan-Avalos5Astrid Poma-Acevedo6Dora Rios-Matos7Andres Agurto-Arteaga8Maria de Grecia Cauti-Mendoza9Norma Perez-Martinez10Gisela Isasi-Rivas11Luis Tataje-Lavanda12Yacory Sernaque-Aguilar13Freddy Ygnacio14Manuel Criollo-Orozco15Edison Huaccachi-Gonzalez16Elmer Delgado-Ccancce17Doris Villanueva-Pérez18Ricardo Montesinos-Millán19Kristel Gutiérrez-Manchay20Katherinne Pauyac-Antezana21Ingrid Ramirez-Ortiz22Stefany Quiñones-Garcia23Yudith Cauna-Orocollo24Katherine Vallejos-Sánchez25Angela Rios-Angulo26Dennis Núñez-Fernández27Mario I. Salguedo-Bohorquez28Julio Ticona29Manolo Fernández-Sánchez30Eliana Icochea31Luis A. Guevara-Sarmiento32Mirko Zimic33COVID-19 Working Group in PerúFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETInstitute of Marine and Environmental Technology, University of Maryland Baltimore CountyFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETFarmacológicos Veterinarios S.A.C. FARVETAbstract The coronavirus disease-19 (COVID-19) pandemic has already claimed millions of lives and remains one of the major catastrophes in the recorded history. While mitigation and control strategies provide short term solutions, vaccines play critical roles in long term control of the disease. Recent emergence of potentially vaccine-resistant and novel variants necessitated testing and deployment of novel technologies that are safe, effective, stable, easy to administer, and inexpensive to produce. Here we developed three recombinant Newcastle disease virus (rNDV) vectored vaccines and assessed their immunogenicity, safety, and protective efficacy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in mice and hamsters. Intranasal administration of rNDV-based vaccine candidates elicited high levels of neutralizing antibodies. Importantly, the nasally administrated vaccine prevented lung damage, and significantly reduced viral load in the respiratory tract of vaccinated animal which was compounded by profound humoral immune responses. Taken together, the presented NDV-based vaccine candidates fully protected animals against SARS-CoV-2 challenge and warrants evaluation in a Phase I human clinical trial as a promising tool in the fight against COVID-19.https://doi.org/10.1038/s41598-022-13560-z |
| spellingShingle | Manolo Fernández Díaz Katherine Calderón Aldo Rojas-Neyra Vikram N. Vakharia Ricardo Choque-Guevara Angela Montalvan-Avalos Astrid Poma-Acevedo Dora Rios-Matos Andres Agurto-Arteaga Maria de Grecia Cauti-Mendoza Norma Perez-Martinez Gisela Isasi-Rivas Luis Tataje-Lavanda Yacory Sernaque-Aguilar Freddy Ygnacio Manuel Criollo-Orozco Edison Huaccachi-Gonzalez Elmer Delgado-Ccancce Doris Villanueva-Pérez Ricardo Montesinos-Millán Kristel Gutiérrez-Manchay Katherinne Pauyac-Antezana Ingrid Ramirez-Ortiz Stefany Quiñones-Garcia Yudith Cauna-Orocollo Katherine Vallejos-Sánchez Angela Rios-Angulo Dennis Núñez-Fernández Mario I. Salguedo-Bohorquez Julio Ticona Manolo Fernández-Sánchez Eliana Icochea Luis A. Guevara-Sarmiento Mirko Zimic COVID-19 Working Group in Perú Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease Scientific Reports |
| title | Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease |
| title_full | Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease |
| title_fullStr | Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease |
| title_full_unstemmed | Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease |
| title_short | Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease |
| title_sort | intranasal vaccination of hamsters with a newcastle disease virus vector expressing the s1 subunit protects animals against sars cov 2 disease |
| url | https://doi.org/10.1038/s41598-022-13560-z |
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