Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP)...
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MDPI AG
2018-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/10/3537 |
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| author | Ruijia Huang Ke Zhang Guoshuai Zhu Zhencheng Sun Songliang He Wenwen Chen |
| author_facet | Ruijia Huang Ke Zhang Guoshuai Zhu Zhencheng Sun Songliang He Wenwen Chen |
| author_sort | Ruijia Huang |
| collection | DOAJ |
| description | Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP) coated microwell plate to perform blocking-free ELISA, in which no nonspecific protein adsorption appears on the GNP layer. If the PEI-GNP coated microwell plate and immobilization of captured antibodies on the plate are prepared in advance, such as using an ELISA kit, the whole ELISA process can be finished in less than 2 h. Meanwhile, we have ensured that the GNP layer can preserve the precision and good linearity of ELISA without causing negative effects on the plate. |
| first_indexed | 2024-04-14T00:58:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T00:58:16Z |
| publishDate | 2018-10-01 |
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| spelling | doaj.art-6971bda864f24c74a0fc045e48ff56032022-12-22T02:21:30ZengMDPI AGSensors1424-82202018-10-011810353710.3390/s18103537s18103537Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell PlateRuijia Huang0Ke Zhang1Guoshuai Zhu2Zhencheng Sun3Songliang He4Wenwen Chen5Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, ChinaGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, ChinaGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, ChinaGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, ChinaGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, ChinaGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, ChinaEnzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP) coated microwell plate to perform blocking-free ELISA, in which no nonspecific protein adsorption appears on the GNP layer. If the PEI-GNP coated microwell plate and immobilization of captured antibodies on the plate are prepared in advance, such as using an ELISA kit, the whole ELISA process can be finished in less than 2 h. Meanwhile, we have ensured that the GNP layer can preserve the precision and good linearity of ELISA without causing negative effects on the plate.http://www.mdpi.com/1424-8220/18/10/3537gold nanoparticlesblocking-freeELISA |
| spellingShingle | Ruijia Huang Ke Zhang Guoshuai Zhu Zhencheng Sun Songliang He Wenwen Chen Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate Sensors gold nanoparticles blocking-free ELISA |
| title | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
| title_full | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
| title_fullStr | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
| title_full_unstemmed | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
| title_short | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
| title_sort | blocking free elisa using a gold nanoparticle layer coated commercial microwell plate |
| topic | gold nanoparticles blocking-free ELISA |
| url | http://www.mdpi.com/1424-8220/18/10/3537 |
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