Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina Faso
Particulate matter (PM) is one of the main air pollutants with 257,000 deaths per year in Africa. Studying their toxic mechanisms of action could provide a better understanding of their effects on the population health. The objective of this study was to describe the PM10 toxic mechanism of action c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2022-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2022/1786810 |
| _version_ | 1797991547734589440 |
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| author | Joelle Nicole Guissou Isabelle Baudrimont Abdoul Karim Ouattara Jacques Simpore Jean Sakande |
| author_facet | Joelle Nicole Guissou Isabelle Baudrimont Abdoul Karim Ouattara Jacques Simpore Jean Sakande |
| author_sort | Joelle Nicole Guissou |
| collection | DOAJ |
| description | Particulate matter (PM) is one of the main air pollutants with 257,000 deaths per year in Africa. Studying their toxic mechanisms of action could provide a better understanding of their effects on the population health. The objective of this study was to describe the PM10 toxic mechanism of action collected in 3 districts of Ouagadougou. Once per month and per site between November 2015 and February 2016, PM10 was sampled for 24 hours using the MiniVol TAS (AirMetrics, Eugene, USA). The collected filters were then stored in Petri dishes at room temperature for in vitro toxicological studies using human pulmonary artery endothelial cells (HPAEC) at the Bordeaux INSERM-U1045 Cardio-thoracic Research Center. The three study districts were classified based on PM10 level (high, intermediate, and low, respectively, for districts 2, 3, and 4). PM10 induced a concentration-dependent decrease in cell viability. A significant decrease in cell viability was observed at 1 µg/cm2, 10 µg/cm2, and 25 µg/cm2 for, respectively, districts 2, 3, and 4. A significant increase in the production of reactive oxygen species (ROS) was observed at 10 µg/cm2 for district 2 versus 5 µg/cm2 and 1 µg/cm2 for districts 3 and 4, respectively. Finally, a significant production of IL-6 was recorded from 5 µg/cm2 for district 4 versus 10 µg/cm2 for districts 2 and 3. Consequently, Ouagadougou is subjected to PM10 pollution, which can induce a significant production of ROS and IL-6 to cause adverse effects on the health of the population. |
| first_indexed | 2024-04-11T08:53:56Z |
| format | Article |
| id | doaj.art-6971ee3d92a9442bb7ed663a0dc18619 |
| institution | Directory Open Access Journal |
| issn | 1687-8205 |
| language | English |
| last_indexed | 2024-04-11T08:53:56Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj.art-6971ee3d92a9442bb7ed663a0dc186192022-12-22T04:33:21ZengHindawi LimitedJournal of Toxicology1687-82052022-01-01202210.1155/2022/1786810Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina FasoJoelle Nicole Guissou0Isabelle Baudrimont1Abdoul Karim Ouattara2Jacques Simpore3Jean Sakande4Laboratory of Environment and Health Toxicology (LATES)Cardiothoracic Research Center of Bordeaux (CRCTB) INSERM-U1045Center for Biomolecular Research Pietro Annigoni (CERBA)Center for Biomolecular Research Pietro Annigoni (CERBA)Health DepartmentParticulate matter (PM) is one of the main air pollutants with 257,000 deaths per year in Africa. Studying their toxic mechanisms of action could provide a better understanding of their effects on the population health. The objective of this study was to describe the PM10 toxic mechanism of action collected in 3 districts of Ouagadougou. Once per month and per site between November 2015 and February 2016, PM10 was sampled for 24 hours using the MiniVol TAS (AirMetrics, Eugene, USA). The collected filters were then stored in Petri dishes at room temperature for in vitro toxicological studies using human pulmonary artery endothelial cells (HPAEC) at the Bordeaux INSERM-U1045 Cardio-thoracic Research Center. The three study districts were classified based on PM10 level (high, intermediate, and low, respectively, for districts 2, 3, and 4). PM10 induced a concentration-dependent decrease in cell viability. A significant decrease in cell viability was observed at 1 µg/cm2, 10 µg/cm2, and 25 µg/cm2 for, respectively, districts 2, 3, and 4. A significant increase in the production of reactive oxygen species (ROS) was observed at 10 µg/cm2 for district 2 versus 5 µg/cm2 and 1 µg/cm2 for districts 3 and 4, respectively. Finally, a significant production of IL-6 was recorded from 5 µg/cm2 for district 4 versus 10 µg/cm2 for districts 2 and 3. Consequently, Ouagadougou is subjected to PM10 pollution, which can induce a significant production of ROS and IL-6 to cause adverse effects on the health of the population.http://dx.doi.org/10.1155/2022/1786810 |
| spellingShingle | Joelle Nicole Guissou Isabelle Baudrimont Abdoul Karim Ouattara Jacques Simpore Jean Sakande Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina Faso Journal of Toxicology |
| title | Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina Faso |
| title_full | Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina Faso |
| title_fullStr | Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina Faso |
| title_full_unstemmed | Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina Faso |
| title_short | Cytotoxicity of Particulate Matter PM10 Samples from Ouagadougou, Burkina Faso |
| title_sort | cytotoxicity of particulate matter pm10 samples from ouagadougou burkina faso |
| url | http://dx.doi.org/10.1155/2022/1786810 |
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