Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia
Abstract Background This study investigated neutrophil activation and neutrophil-derived extracellular traps formation in coronary artery ectasia. Methods We enrolled 90 patients who underwent coronary angiography, and included 30 patients with coronary artery ectasia (CAE), 30 patients with obstruc...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | http://link.springer.com/article/10.1186/s12872-020-01398-0 |
| _version_ | 1818411061198454784 |
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| author | Yuchao Guo Ruifeng Liu Lianfeng Chen Wei Wu Shuyang Zhang |
| author_facet | Yuchao Guo Ruifeng Liu Lianfeng Chen Wei Wu Shuyang Zhang |
| author_sort | Yuchao Guo |
| collection | DOAJ |
| description | Abstract Background This study investigated neutrophil activation and neutrophil-derived extracellular traps formation in coronary artery ectasia. Methods We enrolled 90 patients who underwent coronary angiography, and included 30 patients with coronary artery ectasia (CAE), 30 patients with obstructive coronary artery disease (CAD) and 30 patients with normal coronary arteries (CON). Intra-neutrophil mean myeloperoxidase index (MPXI) was determined using an automated blood cell counter (ADVIA2120 Hematology System). Serum concentrations of plasma adhesion molecules, cytokines, and neutrophil-derived extracellular traps were quantified. Results The intra-neutrophil mean myeloperoxidase index was reduced in CAE patients compared to CAD and CON patients (1.02 ± 3.01, 3.22 ± 3.03, 3.52 ± 4.25, respectively; CAE vs CAD, p = 0.016 and CAE vs CON, p = 0.007). Multiple logistic regression analysis showed that MPXI and dsDNA were independent factors that predicted the presence of CAE. CAE patients had higher levels of plasma adhesion molecules (P-selectin glycoprotein ligand-1, E-selectin, L-selectin) and interleukin 1 beta levels. Neutrophil extracellular trap concentrations were significantly higher in the CAE group compared to CAD and CON patients (284.31(258.33–449.91) ng/mL, 225.12(203.34–257.13) ng/mL, and 247.37(231.04–273.01) ng/mL, respectively; CAE vs CAD, p = 0.000 and CAE vs CON, p = 0.001). Conclusions Peripheral neutrophils from CAE patients were activated and neutrophil extracellular traps were elevated in the plasma. IL-1β and soluble adhesion molecules may be the causal factors for neutrophil activation. |
| first_indexed | 2024-12-14T10:25:25Z |
| format | Article |
| id | doaj.art-697281657e4a42cdb340e337f8122c17 |
| institution | Directory Open Access Journal |
| issn | 1471-2261 |
| language | English |
| last_indexed | 2024-12-14T10:25:25Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-697281657e4a42cdb340e337f8122c172022-12-21T23:06:22ZengBMCBMC Cardiovascular Disorders1471-22612020-03-012011810.1186/s12872-020-01398-0Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasiaYuchao Guo0Ruifeng Liu1Lianfeng Chen2Wei Wu3Shuyang Zhang4Department of Cardiology, Peking Union Medical College & Chinese Academy of Medical Science, Peking Union Medical College HospitalDepartment of Cardiology, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Cardiology, Peking Union Medical College & Chinese Academy of Medical Science, Peking Union Medical College HospitalDepartment of Cardiology, Peking Union Medical College & Chinese Academy of Medical Science, Peking Union Medical College HospitalDepartment of Cardiology, Peking Union Medical College & Chinese Academy of Medical Science, Peking Union Medical College HospitalAbstract Background This study investigated neutrophil activation and neutrophil-derived extracellular traps formation in coronary artery ectasia. Methods We enrolled 90 patients who underwent coronary angiography, and included 30 patients with coronary artery ectasia (CAE), 30 patients with obstructive coronary artery disease (CAD) and 30 patients with normal coronary arteries (CON). Intra-neutrophil mean myeloperoxidase index (MPXI) was determined using an automated blood cell counter (ADVIA2120 Hematology System). Serum concentrations of plasma adhesion molecules, cytokines, and neutrophil-derived extracellular traps were quantified. Results The intra-neutrophil mean myeloperoxidase index was reduced in CAE patients compared to CAD and CON patients (1.02 ± 3.01, 3.22 ± 3.03, 3.52 ± 4.25, respectively; CAE vs CAD, p = 0.016 and CAE vs CON, p = 0.007). Multiple logistic regression analysis showed that MPXI and dsDNA were independent factors that predicted the presence of CAE. CAE patients had higher levels of plasma adhesion molecules (P-selectin glycoprotein ligand-1, E-selectin, L-selectin) and interleukin 1 beta levels. Neutrophil extracellular trap concentrations were significantly higher in the CAE group compared to CAD and CON patients (284.31(258.33–449.91) ng/mL, 225.12(203.34–257.13) ng/mL, and 247.37(231.04–273.01) ng/mL, respectively; CAE vs CAD, p = 0.000 and CAE vs CON, p = 0.001). Conclusions Peripheral neutrophils from CAE patients were activated and neutrophil extracellular traps were elevated in the plasma. IL-1β and soluble adhesion molecules may be the causal factors for neutrophil activation.http://link.springer.com/article/10.1186/s12872-020-01398-0NeutrophilNeutrophil extracellular trapsCoronary artery ectasia |
| spellingShingle | Yuchao Guo Ruifeng Liu Lianfeng Chen Wei Wu Shuyang Zhang Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia BMC Cardiovascular Disorders Neutrophil Neutrophil extracellular traps Coronary artery ectasia |
| title | Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia |
| title_full | Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia |
| title_fullStr | Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia |
| title_full_unstemmed | Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia |
| title_short | Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia |
| title_sort | neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia |
| topic | Neutrophil Neutrophil extracellular traps Coronary artery ectasia |
| url | http://link.springer.com/article/10.1186/s12872-020-01398-0 |
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