Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, <i>Brassica napus</i> L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf v...
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MDPI AG
2022-02-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/3/413 |
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| author | Jing Luo Sha Huang Min Wang Ruimao Zhang Degang Zhao Yuanyu Yang Fang Wang Zhuanzhuan Wang Rong Tang Lulu Wang Huagui Xiao Bin Yang Chao Li |
| author_facet | Jing Luo Sha Huang Min Wang Ruimao Zhang Degang Zhao Yuanyu Yang Fang Wang Zhuanzhuan Wang Rong Tang Lulu Wang Huagui Xiao Bin Yang Chao Li |
| author_sort | Jing Luo |
| collection | DOAJ |
| description | Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, <i>Brassica napus</i> L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dwarfing mechanism of DW871 and provide a theoretical foundation for future applications of the variety, we used transcriptome and proteome sequencing to identify genes and proteins associated with the dwarfing phenotype, using homologous high-stalk material HW871 as a control. By RNA-seq and iTRAQ, we discovered 8665 DEGs and 50 DAPs. Comprehensive transcription and translation level analysis revealed 25 correlations, 23 of which have the same expression trend, involving monolignin synthesis, pectin-lignin assembly, lignification, glucose modification, cell wall composition and architecture, cell morphology, vascular bundle development, and stalk tissue composition and architecture. As a result of these results, we can formulate a hypothesis about the DW871 dwarfing phenotype: plant hormone signal transduction, such as IAA and BRs, is linked to the formation of dwarf phenotypes, and metabolic pathways related to lignin synthesis, such as phenylpropane biosynthesis, also play a role. Our works will contribute to a better understanding of the genes and proteins involved in the rapeseed dwarf phenotype, and we will propose new insights into the dwarfing mechanism of <i>Brassica napus</i> L. |
| first_indexed | 2024-03-09T23:18:34Z |
| format | Article |
| id | doaj.art-6977782a70ba45ce9cb06724f4c57bdc |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T23:18:34Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-6977782a70ba45ce9cb06724f4c57bdc2023-11-23T17:31:35ZengMDPI AGPlants2223-77472022-02-0111341310.3390/plants11030413Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk TissueJing Luo0Sha Huang1Min Wang2Ruimao Zhang3Degang Zhao4Yuanyu Yang5Fang Wang6Zhuanzhuan Wang7Rong Tang8Lulu Wang9Huagui Xiao10Bin Yang11Chao Li12Guizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaSchool of Life Sciences, Guizhou Normal University, Huaxi University City, Gui’an New District, Guiyang 550025, ChinaGuizhou Rapeseed Institute, Guizhou Academy of Agriculture Sciences, No. 111 Duyun Road, Guanshanhu District, Guiyang 520115, ChinaGuizhou Plant Conservation Technology Center, Guizhou Academy of Agricultural Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaGuizhou Oil Crops Institute, Guizhou Academy of Agriculture Sciences, No. 502 Xinzhong Road, Jinxin Community, Huaxi District, Guiyang 550006, ChinaRapeseed is a significant oil-bearing cash crop. As a hybrid crop, <i>Brassica napus</i> L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dwarfing mechanism of DW871 and provide a theoretical foundation for future applications of the variety, we used transcriptome and proteome sequencing to identify genes and proteins associated with the dwarfing phenotype, using homologous high-stalk material HW871 as a control. By RNA-seq and iTRAQ, we discovered 8665 DEGs and 50 DAPs. Comprehensive transcription and translation level analysis revealed 25 correlations, 23 of which have the same expression trend, involving monolignin synthesis, pectin-lignin assembly, lignification, glucose modification, cell wall composition and architecture, cell morphology, vascular bundle development, and stalk tissue composition and architecture. As a result of these results, we can formulate a hypothesis about the DW871 dwarfing phenotype: plant hormone signal transduction, such as IAA and BRs, is linked to the formation of dwarf phenotypes, and metabolic pathways related to lignin synthesis, such as phenylpropane biosynthesis, also play a role. Our works will contribute to a better understanding of the genes and proteins involved in the rapeseed dwarf phenotype, and we will propose new insights into the dwarfing mechanism of <i>Brassica napus</i> L.https://www.mdpi.com/2223-7747/11/3/413<i>Brassica napus</i> L.dwarfing mechanismmultiomicsplant height |
| spellingShingle | Jing Luo Sha Huang Min Wang Ruimao Zhang Degang Zhao Yuanyu Yang Fang Wang Zhuanzhuan Wang Rong Tang Lulu Wang Huagui Xiao Bin Yang Chao Li Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue Plants <i>Brassica napus</i> L. dwarfing mechanism multiomics plant height |
| title | Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
| title_full | Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
| title_fullStr | Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
| title_full_unstemmed | Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
| title_short | Characterization of the Transcriptome and Proteome of <i>Brassica napus</i> Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
| title_sort | characterization of the transcriptome and proteome of i brassica napus i reveals the close relation between dw871 dwarfing phenotype and stalk tissue |
| topic | <i>Brassica napus</i> L. dwarfing mechanism multiomics plant height |
| url | https://www.mdpi.com/2223-7747/11/3/413 |
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