Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.)
The 2-oxoglutarate and Fe (II)-dependent dioxygenase (2ODD-C) family of 2-oxoglutarate-dependent dioxygenases potentially participates in the biosynthesis of various metabolites under various abiotic stresses. However, there is scarce information on the expression profiles and roles of <i>2ODD...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/6/1302 |
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| author | Jingxue Han Xiaojing Wang Suzhen Niu |
| author_facet | Jingxue Han Xiaojing Wang Suzhen Niu |
| author_sort | Jingxue Han |
| collection | DOAJ |
| description | The 2-oxoglutarate and Fe (II)-dependent dioxygenase (2ODD-C) family of 2-oxoglutarate-dependent dioxygenases potentially participates in the biosynthesis of various metabolites under various abiotic stresses. However, there is scarce information on the expression profiles and roles of <i>2ODD-C</i> genes in <i>Camellia sinensis</i>. We identified 153 <i>Cs2ODD-C</i> genes from <i>C. sinensis</i>, and they were distributed unevenly on 15 chromosomes. According to the phylogenetic tree topology, these genes were divided into 21 groups distinguished by conserved motifs and an intron/exon structure. Gene-duplication analyses revealed that 75 <i>Cs2ODD-C</i> genes were expanded and retained after WGD/segmental and tandem duplications. The expression profiles of <i>Cs2ODD-C</i> genes were explored under methyl jasmonate (MeJA), polyethylene glycol (PEG), and salt (NaCl) stress treatments. The expression analysis showed that 14, 13, and 49 <i>Cs2ODD-C</i> genes displayed the same expression pattern under MeJA and PEG treatments, MeJA and NaCl treatments, and PEG and NaCl treatments, respectively. A further analysis showed that two genes, <i>Cs2ODD-C36</i> and <i>Cs2ODD-C21</i>, were significantly upregulated and downregulated after MeJA, PEG, and NaCl treatments, indicating that these two genes played positive and negative roles in enhancing the multi-stress tolerance. These results provide candidate genes for the use of genetic engineering technology to modify plants by enhancing multi-stress tolerance to promote phytoremediation efficiency. |
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| last_indexed | 2024-03-11T06:00:14Z |
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| spelling | doaj.art-697b6c7c86ce45d5a2de3b6d414406f22023-11-17T13:21:57ZengMDPI AGPlants2223-77472023-03-01126130210.3390/plants12061302Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.)Jingxue Han0Xiaojing Wang1Suzhen Niu2College of Tea Science, Guizhou University, Guiyang 550025, ChinaCollege of Tea Science, Guizhou University, Guiyang 550025, ChinaCollege of Tea Science, Guizhou University, Guiyang 550025, ChinaThe 2-oxoglutarate and Fe (II)-dependent dioxygenase (2ODD-C) family of 2-oxoglutarate-dependent dioxygenases potentially participates in the biosynthesis of various metabolites under various abiotic stresses. However, there is scarce information on the expression profiles and roles of <i>2ODD-C</i> genes in <i>Camellia sinensis</i>. We identified 153 <i>Cs2ODD-C</i> genes from <i>C. sinensis</i>, and they were distributed unevenly on 15 chromosomes. According to the phylogenetic tree topology, these genes were divided into 21 groups distinguished by conserved motifs and an intron/exon structure. Gene-duplication analyses revealed that 75 <i>Cs2ODD-C</i> genes were expanded and retained after WGD/segmental and tandem duplications. The expression profiles of <i>Cs2ODD-C</i> genes were explored under methyl jasmonate (MeJA), polyethylene glycol (PEG), and salt (NaCl) stress treatments. The expression analysis showed that 14, 13, and 49 <i>Cs2ODD-C</i> genes displayed the same expression pattern under MeJA and PEG treatments, MeJA and NaCl treatments, and PEG and NaCl treatments, respectively. A further analysis showed that two genes, <i>Cs2ODD-C36</i> and <i>Cs2ODD-C21</i>, were significantly upregulated and downregulated after MeJA, PEG, and NaCl treatments, indicating that these two genes played positive and negative roles in enhancing the multi-stress tolerance. These results provide candidate genes for the use of genetic engineering technology to modify plants by enhancing multi-stress tolerance to promote phytoremediation efficiency.https://www.mdpi.com/2223-7747/12/6/1302<i>C. sinensis</i><i>Cs2ODD-C</i> genesphylogenetic analysisexpression profileabiotic stresses |
| spellingShingle | Jingxue Han Xiaojing Wang Suzhen Niu Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.) Plants <i>C. sinensis</i> <i>Cs2ODD-C</i> genes phylogenetic analysis expression profile abiotic stresses |
| title | Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.) |
| title_full | Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.) |
| title_fullStr | Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.) |
| title_full_unstemmed | Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.) |
| title_short | Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in <i>Camellia sinensis</i> (L.) |
| title_sort | genome wide identification of 2 oxoglutarate and fe ii dependent dioxygenase 2odd c family genes and expression profiles under different abiotic stresses in i camellia sinensis i l |
| topic | <i>C. sinensis</i> <i>Cs2ODD-C</i> genes phylogenetic analysis expression profile abiotic stresses |
| url | https://www.mdpi.com/2223-7747/12/6/1302 |
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