Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity
Persimmon (<i>Diospyros kaki</i>), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals. However, its high-molecular-weight compounds, particularly polysaccharides,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/6/1600 |
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| author | Ki Cheol Hwang Hyun Young Shin Woo Jung Kim Mi Suk Seo Hoon Kim |
| author_facet | Ki Cheol Hwang Hyun Young Shin Woo Jung Kim Mi Suk Seo Hoon Kim |
| author_sort | Ki Cheol Hwang |
| collection | DOAJ |
| description | Persimmon (<i>Diospyros kaki</i>), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals. However, its high-molecular-weight compounds, particularly polysaccharides, have not been extensively studied. In this study, <i>D. kaki</i> extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system. DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L. Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon. Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues. These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries. |
| first_indexed | 2024-03-10T13:15:26Z |
| format | Article |
| id | doaj.art-697c35f5677143adb4435f6b0356295e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:15:26Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-697c35f5677143adb4435f6b0356295e2023-11-21T10:24:11ZengMDPI AGMolecules1420-30492021-03-01266160010.3390/molecules26061600Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle ActivityKi Cheol Hwang0Hyun Young Shin1Woo Jung Kim2Mi Suk Seo3Hoon Kim4Rafarophe Co, Venture Research Center Suite 605, 194-41, Osongsaengmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju 28160, KoreaDepartment of Integrated Biomedical and Life Science, Korea University, Seoul 02841, KoreaBiocenter, Gyeonggido Business and Science Accelerator, Gwanggyo-ro 147, Yeongtong-gu, Suwon 16229, KoreaBiocenter, Gyeonggido Business and Science Accelerator, Gwanggyo-ro 147, Yeongtong-gu, Suwon 16229, KoreaSkin-Biotechnology Center, Kyung Hee University, Gwanggyo-ro 147, Yeongtong-gu, Suwon 16229, KoreaPersimmon (<i>Diospyros kaki</i>), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals. However, its high-molecular-weight compounds, particularly polysaccharides, have not been extensively studied. In this study, <i>D. kaki</i> extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system. DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L. Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon. Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues. These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.https://www.mdpi.com/1420-3049/26/6/1600<i>Diospyros kaki</i>persimmon fruitcosmeceuticalskin functionpolysaccharide |
| spellingShingle | Ki Cheol Hwang Hyun Young Shin Woo Jung Kim Mi Suk Seo Hoon Kim Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity Molecules <i>Diospyros kaki</i> persimmon fruit cosmeceutical skin function polysaccharide |
| title | Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity |
| title_full | Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity |
| title_fullStr | Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity |
| title_full_unstemmed | Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity |
| title_short | Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity |
| title_sort | effects of a high molecular weight polysaccharides isolated from korean persimmon on the antioxidant anti inflammatory and antiwrinkle activity |
| topic | <i>Diospyros kaki</i> persimmon fruit cosmeceutical skin function polysaccharide |
| url | https://www.mdpi.com/1420-3049/26/6/1600 |
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