Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpio
Rab7 is a small GTPase that regulates vesicular traffic from early to late endosomal stages of the endocytic pathway. In this study, full-length cDNA sequences of Rab7 were cloned from Yellow River Carp Cyprinus carpio, which were designated as CcRab7, and its function during pathogen infection was...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003538 |
| _version_ | 1797388813507493888 |
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| author | Guilan Di Ning Wang Xiaoyue Shen Di Lan Yunlong Wu Xianghui Kong Xinhua Chen |
| author_facet | Guilan Di Ning Wang Xiaoyue Shen Di Lan Yunlong Wu Xianghui Kong Xinhua Chen |
| author_sort | Guilan Di |
| collection | DOAJ |
| description | Rab7 is a small GTPase that regulates vesicular traffic from early to late endosomal stages of the endocytic pathway. In this study, full-length cDNA sequences of Rab7 were cloned from Yellow River Carp Cyprinus carpio, which were designated as CcRab7, and its function during pathogen infection was also investigated. The results showed that the full-length cDNA of CcRab7 was composed of 1511 bp, and open reading frame is 618 bp encoding 205 amino acids with 23.10 kDa. The deduced amino acid sequence of CcRab7 shared high similarity to that of Pimephales promelas (99.02%). In this paper, real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression levels in 11 tissues and organs of healthy Yellow River Carp, and the tissue distribution of CcRab7 as well as its defense mechanism against Spring viremia of carp virus (SVCV) and Aeromonas hydrophila in Yellow River Carp C. carpio was investigated. The results revealed that the mRNA transcripts of CcRab7 were widely detected in various tissues of C. carpio, with the highest expression levels in head kidney and blood, indicating that CcRab7 was involved in the innate immune process. Yellow River Carp was immunized with A. hydrophila and SVCV respectively, and the expression changes of CcRab7 were detected in gill, spleen, liver and skin of Yellow River Carp. The mRNA transcripts of CcRab7 in the main organs (gill, and liver, skin and spleen) were significantly affected by SVCV and A. hydrophila infection. The results showed that the CcRab7 expression levels was obviously up-regulated at different time points. The eukaryotic recombinant plasmids of CcRab7 and pEGFP-N3 transfected into Grass carp ovary cell lines (GCO) cells for subcellular localization. The results provide a basis for further investigation into the immunological role of CcRab7 in Yellow River Carp. |
| first_indexed | 2024-03-08T22:46:16Z |
| format | Article |
| id | doaj.art-697cbfd56fde42c2a64252df68201c27 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:16Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-697cbfd56fde42c2a64252df68201c272023-12-17T06:39:49ZengElsevierAquaculture Reports2352-51342023-12-0133101814Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpioGuilan Di0Ning Wang1Xiaoyue Shen2Di Lan3Yunlong Wu4Xianghui Kong5Xinhua Chen6State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; College of Fisheries, Henan Normal University, Xinxiang 453007, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, ChinaState Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaDepartment of Biotechnology, Fuzhou Institute of Technology, 350506, ChinaState Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, China; Correspondence to: Xianghui Kong, College of Fisheries, Henan Normal University, No. 46, Jianshe Road, Xinxiang 453007, China.State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fuzhou Institute of Oceanography, Fuzhou 350108, China; Correspondence to: Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.Rab7 is a small GTPase that regulates vesicular traffic from early to late endosomal stages of the endocytic pathway. In this study, full-length cDNA sequences of Rab7 were cloned from Yellow River Carp Cyprinus carpio, which were designated as CcRab7, and its function during pathogen infection was also investigated. The results showed that the full-length cDNA of CcRab7 was composed of 1511 bp, and open reading frame is 618 bp encoding 205 amino acids with 23.10 kDa. The deduced amino acid sequence of CcRab7 shared high similarity to that of Pimephales promelas (99.02%). In this paper, real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression levels in 11 tissues and organs of healthy Yellow River Carp, and the tissue distribution of CcRab7 as well as its defense mechanism against Spring viremia of carp virus (SVCV) and Aeromonas hydrophila in Yellow River Carp C. carpio was investigated. The results revealed that the mRNA transcripts of CcRab7 were widely detected in various tissues of C. carpio, with the highest expression levels in head kidney and blood, indicating that CcRab7 was involved in the innate immune process. Yellow River Carp was immunized with A. hydrophila and SVCV respectively, and the expression changes of CcRab7 were detected in gill, spleen, liver and skin of Yellow River Carp. The mRNA transcripts of CcRab7 in the main organs (gill, and liver, skin and spleen) were significantly affected by SVCV and A. hydrophila infection. The results showed that the CcRab7 expression levels was obviously up-regulated at different time points. The eukaryotic recombinant plasmids of CcRab7 and pEGFP-N3 transfected into Grass carp ovary cell lines (GCO) cells for subcellular localization. The results provide a basis for further investigation into the immunological role of CcRab7 in Yellow River Carp.http://www.sciencedirect.com/science/article/pii/S2352513423003538Yellow River Carp Cyprinus carpioCcRab7Molecular characterizationImmune function |
| spellingShingle | Guilan Di Ning Wang Xiaoyue Shen Di Lan Yunlong Wu Xianghui Kong Xinhua Chen Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpio Aquaculture Reports Yellow River Carp Cyprinus carpio CcRab7 Molecular characterization Immune function |
| title | Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpio |
| title_full | Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpio |
| title_fullStr | Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpio |
| title_full_unstemmed | Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpio |
| title_short | Identification and molecular characterization of Rab7 form Yellow River Carp Cyprinus carpio |
| title_sort | identification and molecular characterization of rab7 form yellow river carp cyprinus carpio |
| topic | Yellow River Carp Cyprinus carpio CcRab7 Molecular characterization Immune function |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003538 |
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