Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6
<p>Abstract</p> <p>Background</p> <p>Human kallikrein-related peptidase 6 (KLK6) has been implicated in various types of cancer and in neurodegenerative and demyelinating diseases including multiple sclerosis. Further, anti-KLK6 antibodies attenuated disease manifestati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-12-01
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| Series: | Chemistry Central Journal |
| Subjects: | |
| Online Access: | http://journal.chemistrycentral.com/content/6/1/148 |
| _version_ | 1830232958809669632 |
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| author | Sotiropoulou Georgia Pampalakis Georgios Prosnikli Evangelia Evangelatos Gregory P Livaniou Evangelia |
| author_facet | Sotiropoulou Georgia Pampalakis Georgios Prosnikli Evangelia Evangelatos Gregory P Livaniou Evangelia |
| author_sort | Sotiropoulou Georgia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Human kallikrein-related peptidase 6 (KLK6) has been implicated in various types of cancer and in neurodegenerative and demyelinating diseases including multiple sclerosis. Further, anti-KLK6 antibodies attenuated disease manifestations in the mouse model of multiple sclerosis. Availability of specific antibodies against KLK6 is fundamental to the development of improved diagnostic and/or immunotherapeutic applications. Here, we exploited the enhanced immunogenicity of mammalian proteins in avian species to generate a polyclonal antibody against KLK6.</p> <p>Results</p> <p>Chicken were immunized with recombinant KLK6 and antibodies Y (IgYs) were purified from egg yolk with a simple procedure and evaluated for KLK6 detection by ELISA and Western blot using recombinant proteins and human cell lysates and supernatants. The anti-KLK6 Y polyclonal exhibited high affinity for KLK6 with a detection limit of 30 fmol. On the other hand, the widely used rabbit polyclonal antibody that was raised against the same recombinant KLK6 had a detection limit of 300 fmol. Moreover, the IgYs did not display any crossreactivity with recombinant KLKs or endogenous KLKs and other cellular proteins.</p> <p>Conclusions</p> <p>Based on its high specificity and sensitivity the developed anti-KLK6 IgY is expected to aid the development of improved diagnostic tools for the detection of KLK6 in biological and clinical samples.</p> |
| first_indexed | 2024-12-18T11:46:54Z |
| format | Article |
| id | doaj.art-697dab148e72489e881faf9686d7e724 |
| institution | Directory Open Access Journal |
| issn | 1752-153X |
| language | English |
| last_indexed | 2024-12-18T11:46:54Z |
| publishDate | 2012-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Chemistry Central Journal |
| spelling | doaj.art-697dab148e72489e881faf9686d7e7242022-12-21T21:09:16ZengBMCChemistry Central Journal1752-153X2012-12-016114810.1186/1752-153X-6-148Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6Sotiropoulou GeorgiaPampalakis GeorgiosProsnikli EvangeliaEvangelatos Gregory PLivaniou Evangelia<p>Abstract</p> <p>Background</p> <p>Human kallikrein-related peptidase 6 (KLK6) has been implicated in various types of cancer and in neurodegenerative and demyelinating diseases including multiple sclerosis. Further, anti-KLK6 antibodies attenuated disease manifestations in the mouse model of multiple sclerosis. Availability of specific antibodies against KLK6 is fundamental to the development of improved diagnostic and/or immunotherapeutic applications. Here, we exploited the enhanced immunogenicity of mammalian proteins in avian species to generate a polyclonal antibody against KLK6.</p> <p>Results</p> <p>Chicken were immunized with recombinant KLK6 and antibodies Y (IgYs) were purified from egg yolk with a simple procedure and evaluated for KLK6 detection by ELISA and Western blot using recombinant proteins and human cell lysates and supernatants. The anti-KLK6 Y polyclonal exhibited high affinity for KLK6 with a detection limit of 30 fmol. On the other hand, the widely used rabbit polyclonal antibody that was raised against the same recombinant KLK6 had a detection limit of 300 fmol. Moreover, the IgYs did not display any crossreactivity with recombinant KLKs or endogenous KLKs and other cellular proteins.</p> <p>Conclusions</p> <p>Based on its high specificity and sensitivity the developed anti-KLK6 IgY is expected to aid the development of improved diagnostic tools for the detection of KLK6 in biological and clinical samples.</p>http://journal.chemistrycentral.com/content/6/1/148Kallikrein-related peptidase 6(KLK6)IgYImmunoassays |
| spellingShingle | Sotiropoulou Georgia Pampalakis Georgios Prosnikli Evangelia Evangelatos Gregory P Livaniou Evangelia Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6 Chemistry Central Journal Kallikrein-related peptidase 6(KLK6) IgY Immunoassays |
| title | Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6 |
| title_full | Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6 |
| title_fullStr | Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6 |
| title_full_unstemmed | Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6 |
| title_short | Development and immunochemical evaluation of a novel chicken IgY antibody specific for KLK6 |
| title_sort | development and immunochemical evaluation of a novel chicken igy antibody specific for klk6 |
| topic | Kallikrein-related peptidase 6(KLK6) IgY Immunoassays |
| url | http://journal.chemistrycentral.com/content/6/1/148 |
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