Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry
Colletotrichum siamense infected the petiole and crown of strawberry (Fragaria × ananassa), and this anthracnose threatens strawberry production heavily in Hubei province. Characteristic the variation of transcript caused by C. siamense is important for anthracnose control of strawberry. In this st...
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| Format: | Article |
| Language: | English |
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AcademicPres
2022-09-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12791 |
| _version_ | 1811181739346755584 |
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| author | Rong ZHENG Bo SHU Chun LUO |
| author_facet | Rong ZHENG Bo SHU Chun LUO |
| author_sort | Rong ZHENG |
| collection | DOAJ |
| description |
Colletotrichum siamense infected the petiole and crown of strawberry (Fragaria × ananassa), and this anthracnose threatens strawberry production heavily in Hubei province. Characteristic the variation of transcript caused by C. siamense is important for anthracnose control of strawberry. In this study, RNA-seq analysis was used to monitor the strawberry transcripts in response of C. siamense at 0- and 6-days post-inoculation based on lesion measurement and hypha observation. The number of clean reads achieved for each sample was ranged from 5.68 to 8.12 Gb. The 0DPM vs 0DPI group had seven significantly differentially expressed transcripts, whereas the 6DPM vs 6DPI group exhibited 12,097 significantly differentially expressed transcripts. The differentially expressed transcripts of 6DPM vs 6DPI group were enriched in ‘metabolic process’ and ‘biological process’ in the biological process category and ‘oxidoreductase activity’ in the molecular function category by GO analysis. While ‘Starch and sucrose metabolism’, ‘Plant hormone signal transduction’ and ‘Phenylpropanoid biosynthesis’ pathways were significantly enriched by KEGG analysis. The expression of MYC2 in JA, the ETR in Eth and, the SnRK2 and ABF in ABA signal transduction pathways were promoted, whereas expression of the PR1 in SA, JZA1 in JA, and ERF in Eth signal transduction pathways were inhibited. In addition, the transcripts that encode enzymes involved in the ‘Phenylpropanoid biosynthesis’ pathway, such as PAL, CYP73A, CSE, 4CL, HTC, GSE and COMT were all inhibited showed by RNA-seq and qRT-PCR. This study provided basic information of strawberry responding to C. siamense infection.
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| first_indexed | 2024-04-11T09:22:35Z |
| format | Article |
| id | doaj.art-6981cc9ca1004008968cef45d8cc498c |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-04-11T09:22:35Z |
| publishDate | 2022-09-01 |
| publisher | AcademicPres |
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| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-6981cc9ca1004008968cef45d8cc498c2022-12-22T04:32:09ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092022-09-0150310.15835/nbha50312791Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberryRong ZHENG0Bo SHU1Chun LUO2Yangtze University, College of Horticulture and Gardening, 1 Nanhuan road, JingzhouYangtze University, College of Horticulture and Gardening, 1 Nanhuan road, JingzhouYangtze University, College of Horticulture and Gardening, 1 Nanhuan road, Jingzhou Colletotrichum siamense infected the petiole and crown of strawberry (Fragaria × ananassa), and this anthracnose threatens strawberry production heavily in Hubei province. Characteristic the variation of transcript caused by C. siamense is important for anthracnose control of strawberry. In this study, RNA-seq analysis was used to monitor the strawberry transcripts in response of C. siamense at 0- and 6-days post-inoculation based on lesion measurement and hypha observation. The number of clean reads achieved for each sample was ranged from 5.68 to 8.12 Gb. The 0DPM vs 0DPI group had seven significantly differentially expressed transcripts, whereas the 6DPM vs 6DPI group exhibited 12,097 significantly differentially expressed transcripts. The differentially expressed transcripts of 6DPM vs 6DPI group were enriched in ‘metabolic process’ and ‘biological process’ in the biological process category and ‘oxidoreductase activity’ in the molecular function category by GO analysis. While ‘Starch and sucrose metabolism’, ‘Plant hormone signal transduction’ and ‘Phenylpropanoid biosynthesis’ pathways were significantly enriched by KEGG analysis. The expression of MYC2 in JA, the ETR in Eth and, the SnRK2 and ABF in ABA signal transduction pathways were promoted, whereas expression of the PR1 in SA, JZA1 in JA, and ERF in Eth signal transduction pathways were inhibited. In addition, the transcripts that encode enzymes involved in the ‘Phenylpropanoid biosynthesis’ pathway, such as PAL, CYP73A, CSE, 4CL, HTC, GSE and COMT were all inhibited showed by RNA-seq and qRT-PCR. This study provided basic information of strawberry responding to C. siamense infection. https://www.notulaebotanicae.ro/index.php/nbha/article/view/12791‘Benihoppe’ strawberrygene expressionpetiolephenylpropanoidsignal transduction |
| spellingShingle | Rong ZHENG Bo SHU Chun LUO Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry Notulae Botanicae Horti Agrobotanici Cluj-Napoca ‘Benihoppe’ strawberry gene expression petiole phenylpropanoid signal transduction |
| title | Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry |
| title_full | Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry |
| title_fullStr | Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry |
| title_full_unstemmed | Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry |
| title_short | Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry |
| title_sort | colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry |
| topic | ‘Benihoppe’ strawberry gene expression petiole phenylpropanoid signal transduction |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12791 |
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