Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies
During development of yeast colonies, various cell subpopulations form, which differ in their properties and specifically localize within the structure. Three branches of mitochondrial retrograde (RTG) signaling play a role in colony development and differentiation, each of them activating the produ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/11/5597 |
| _version_ | 1797532730598096896 |
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| author | Vítězslav Plocek Kristýna Fadrhonc Jana Maršíková Libuše Váchová Alexandra Pokorná Otakar Hlaváček Derek Wilkinson Zdena Palková |
| author_facet | Vítězslav Plocek Kristýna Fadrhonc Jana Maršíková Libuše Váchová Alexandra Pokorná Otakar Hlaváček Derek Wilkinson Zdena Palková |
| author_sort | Vítězslav Plocek |
| collection | DOAJ |
| description | During development of yeast colonies, various cell subpopulations form, which differ in their properties and specifically localize within the structure. Three branches of mitochondrial retrograde (RTG) signaling play a role in colony development and differentiation, each of them activating the production of specific markers in different cell types. Here, aiming to identify proteins and processes controlled by the RTG pathway, we analyzed proteomes of individual cell subpopulations from colonies of strains, mutated in genes of the RTG pathway. Resulting data, along with microscopic analyses revealed that the RTG pathway predominantly regulates processes in U cells, long-lived cells with unique properties, which are localized in upper colony regions. Rtg proteins therein activate processes leading to amino acid biosynthesis, including transport of metabolic intermediates between compartments, but also repress expression of mitochondrial ribosome components, thus possibly contributing to reduced mitochondrial translation in U cells. The results reveal the RTG pathway’s role in activating metabolic processes, important in U cell adaptation to altered nutritional conditions. They also point to the important role of Rtg regulators in repressing mitochondrial activity in U cells. |
| first_indexed | 2024-03-10T11:03:27Z |
| format | Article |
| id | doaj.art-6985e1d50f6e41d69b9bb8638b01c998 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:03:27Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6985e1d50f6e41d69b9bb8638b01c9982023-11-21T21:18:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012211559710.3390/ijms22115597Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast ColoniesVítězslav Plocek0Kristýna Fadrhonc1Jana Maršíková2Libuše Váchová3Alexandra Pokorná4Otakar Hlaváček5Derek Wilkinson6Zdena Palková7Department of Genetics and Microbiology, Faculty of Science, Charles University, BIOCEV, 12800 Prague, Czech RepublicDepartment of Genetics and Microbiology, Faculty of Science, Charles University, BIOCEV, 12800 Prague, Czech RepublicDepartment of Genetics and Microbiology, Faculty of Science, Charles University, BIOCEV, 12800 Prague, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, BIOCEV, 14220 Prague, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, BIOCEV, 14220 Prague, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, BIOCEV, 14220 Prague, Czech RepublicDepartment of Genetics and Microbiology, Faculty of Science, Charles University, BIOCEV, 12800 Prague, Czech RepublicDepartment of Genetics and Microbiology, Faculty of Science, Charles University, BIOCEV, 12800 Prague, Czech RepublicDuring development of yeast colonies, various cell subpopulations form, which differ in their properties and specifically localize within the structure. Three branches of mitochondrial retrograde (RTG) signaling play a role in colony development and differentiation, each of them activating the production of specific markers in different cell types. Here, aiming to identify proteins and processes controlled by the RTG pathway, we analyzed proteomes of individual cell subpopulations from colonies of strains, mutated in genes of the RTG pathway. Resulting data, along with microscopic analyses revealed that the RTG pathway predominantly regulates processes in U cells, long-lived cells with unique properties, which are localized in upper colony regions. Rtg proteins therein activate processes leading to amino acid biosynthesis, including transport of metabolic intermediates between compartments, but also repress expression of mitochondrial ribosome components, thus possibly contributing to reduced mitochondrial translation in U cells. The results reveal the RTG pathway’s role in activating metabolic processes, important in U cell adaptation to altered nutritional conditions. They also point to the important role of Rtg regulators in repressing mitochondrial activity in U cells.https://www.mdpi.com/1422-0067/22/11/5597mitochondrial retrograde signalingyeast coloniescolony development and differentiation<i>Saccharomyces cerevisiae</i>proteomic analysis |
| spellingShingle | Vítězslav Plocek Kristýna Fadrhonc Jana Maršíková Libuše Váchová Alexandra Pokorná Otakar Hlaváček Derek Wilkinson Zdena Palková Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies International Journal of Molecular Sciences mitochondrial retrograde signaling yeast colonies colony development and differentiation <i>Saccharomyces cerevisiae</i> proteomic analysis |
| title | Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies |
| title_full | Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies |
| title_fullStr | Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies |
| title_full_unstemmed | Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies |
| title_short | Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies |
| title_sort | mitochondrial retrograde signaling contributes to metabolic differentiation in yeast colonies |
| topic | mitochondrial retrograde signaling yeast colonies colony development and differentiation <i>Saccharomyces cerevisiae</i> proteomic analysis |
| url | https://www.mdpi.com/1422-0067/22/11/5597 |
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