Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive
Continuous culture study of the ruminal bacterium Megasphaera elsdenii using lactate as carbon source is reported. Butyrate, the minor product in batch culture, was the main product in continuous culture. Under steady state conditions the biomass concentration was increased from 0.46 to 0.72 g l-1....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2001-06-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200011&tlng=en |
| _version_ | 1798021718992748544 |
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| author | Oscar Soto-Cruz Rafael Chávez-Rivera Gerardo Saucedo-Castañeda |
| author_facet | Oscar Soto-Cruz Rafael Chávez-Rivera Gerardo Saucedo-Castañeda |
| author_sort | Oscar Soto-Cruz |
| collection | DOAJ |
| description | Continuous culture study of the ruminal bacterium Megasphaera elsdenii using lactate as carbon source is reported. Butyrate, the minor product in batch culture, was the main product in continuous culture. Under steady state conditions the biomass concentration was increased from 0.46 to 0.72 g l-1. Addition of Yea Sacc did not result an increased availability of carbon. The produced amounts of valerate and CO2 were calculated by using a combined carbon and degree of reduction balances. According to these results, the lactate catabolism of M. elsdenii was changed by the presence of yeast additive, yielding more butyrate and diminishing the production of valerate, while acetate and propionate production was not affected. Results reported in this work suggested that soluble compounds present in the yeast additive filtrate enhanced the anabolism of M. elsdenii and modified the carbon fluxes through its pathways by increasing butyrate and decreasing valerate production in continuous culture. |
| first_indexed | 2024-04-11T17:18:03Z |
| format | Article |
| id | doaj.art-698a508239c24646a2033bd3e2a4e329 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T17:18:03Z |
| publishDate | 2001-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-698a508239c24646a2033bd3e2a4e3292022-12-22T04:12:34ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242001-06-0144217918410.1590/S1516-89132001000200011Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additiveOscar Soto-Cruz0Rafael Chávez-Rivera1Gerardo Saucedo-Castañeda2Autonomous Metropolitan UniversityAutonomous Metropolitan UniversityAutonomous Metropolitan UniversityContinuous culture study of the ruminal bacterium Megasphaera elsdenii using lactate as carbon source is reported. Butyrate, the minor product in batch culture, was the main product in continuous culture. Under steady state conditions the biomass concentration was increased from 0.46 to 0.72 g l-1. Addition of Yea Sacc did not result an increased availability of carbon. The produced amounts of valerate and CO2 were calculated by using a combined carbon and degree of reduction balances. According to these results, the lactate catabolism of M. elsdenii was changed by the presence of yeast additive, yielding more butyrate and diminishing the production of valerate, while acetate and propionate production was not affected. Results reported in this work suggested that soluble compounds present in the yeast additive filtrate enhanced the anabolism of M. elsdenii and modified the carbon fluxes through its pathways by increasing butyrate and decreasing valerate production in continuous culture.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200011&tlng=enMegasphaera elsdeniibutyrateyeast additivecontinuous culture |
| spellingShingle | Oscar Soto-Cruz Rafael Chávez-Rivera Gerardo Saucedo-Castañeda Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive Brazilian Archives of Biology and Technology Megasphaera elsdenii butyrate yeast additive continuous culture |
| title | Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive |
| title_full | Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive |
| title_fullStr | Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive |
| title_full_unstemmed | Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive |
| title_short | Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive |
| title_sort | stimulation of the megasphaera elsdenii s butyrate production in continuous culture by a yeast additive |
| topic | Megasphaera elsdenii butyrate yeast additive continuous culture |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200011&tlng=en |
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