Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ Emission
Manure spreading from farm animals can release antibiotic-resistant bacteria (ARB) carrying antimicrobial resistance genes (ARGs) into the air, posing a potential threat to human and animal health due to the intensive use of antibiotics in the livestock industry. This study analyzed the effect of di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/7/1797 |
| _version_ | 1797588092644753408 |
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| author | Mahsa Baghdadi Patrick Brassard Stéphane Godbout Valérie Létourneau Nathalie Turgeon Florent Rossi Émie Lachance Marc Veillette Marie-Lou Gaucher Caroline Duchaine |
| author_facet | Mahsa Baghdadi Patrick Brassard Stéphane Godbout Valérie Létourneau Nathalie Turgeon Florent Rossi Émie Lachance Marc Veillette Marie-Lou Gaucher Caroline Duchaine |
| author_sort | Mahsa Baghdadi |
| collection | DOAJ |
| description | Manure spreading from farm animals can release antibiotic-resistant bacteria (ARB) carrying antimicrobial resistance genes (ARGs) into the air, posing a potential threat to human and animal health due to the intensive use of antibiotics in the livestock industry. This study analyzed the effect of different manure types and spreading methods on airborne bacterial emissions and antibiotic resistance genes in a controlled setting. Cow, poultry manure, and pig slurry were spread in a confined environment using two types of spreaders (splash plate and dribble bar), and the resulting emissions were collected before, during, and after spreading using high-volume air samplers coupled to a particle counter. Total bacteria, fecal indicators, and a total of 38 different subtypes of ARGs were further quantified by qPCR. Spreading poultry manure resulted in the highest emission rates of total bacteria (10<sup>11</sup> 16S gene copies/kg manure spread), <i>Archaea</i> (10<sup>6</sup> 16S gene copies/kg manure), <i>Enterococcus</i> (10<sup>5</sup> 16S gene copies/kg manure), and <i>E. coli</i> (10<sup>4</sup> 16S gene copies/kg manure), followed by cow manure and pig slurry with splash plates and the dribble bar. Manure spreading was associated with the highest rates of airborne aminoglycoside genes for cow and poultry (10<sup>6</sup> gene copies/kg manure), followed by pig slurry (10<sup>4</sup> gene copies/kg manure). This study shows that the type of manure and spreading equipment can affect the emission rates of airborne bacteria, and ARGs. |
| first_indexed | 2024-03-11T00:48:50Z |
| format | Article |
| id | doaj.art-698f864d9d664114a214144897521bc4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T00:48:50Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-698f864d9d664114a214144897521bc42023-11-18T20:36:35ZengMDPI AGMicroorganisms2076-26072023-07-01117179710.3390/microorganisms11071797Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ EmissionMahsa Baghdadi0Patrick Brassard1Stéphane Godbout2Valérie Létourneau3Nathalie Turgeon4Florent Rossi5Émie Lachance6Marc Veillette7Marie-Lou Gaucher8Caroline Duchaine9Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaInstitut de Recherche et de Développement en Agroenvironnement, Québec, QC G1P 3W8, CanadaInstitut de Recherche et de Développement en Agroenvironnement, Québec, QC G1P 3W8, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaChaire de Recherche en Salubrité des Viandes, Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaManure spreading from farm animals can release antibiotic-resistant bacteria (ARB) carrying antimicrobial resistance genes (ARGs) into the air, posing a potential threat to human and animal health due to the intensive use of antibiotics in the livestock industry. This study analyzed the effect of different manure types and spreading methods on airborne bacterial emissions and antibiotic resistance genes in a controlled setting. Cow, poultry manure, and pig slurry were spread in a confined environment using two types of spreaders (splash plate and dribble bar), and the resulting emissions were collected before, during, and after spreading using high-volume air samplers coupled to a particle counter. Total bacteria, fecal indicators, and a total of 38 different subtypes of ARGs were further quantified by qPCR. Spreading poultry manure resulted in the highest emission rates of total bacteria (10<sup>11</sup> 16S gene copies/kg manure spread), <i>Archaea</i> (10<sup>6</sup> 16S gene copies/kg manure), <i>Enterococcus</i> (10<sup>5</sup> 16S gene copies/kg manure), and <i>E. coli</i> (10<sup>4</sup> 16S gene copies/kg manure), followed by cow manure and pig slurry with splash plates and the dribble bar. Manure spreading was associated with the highest rates of airborne aminoglycoside genes for cow and poultry (10<sup>6</sup> gene copies/kg manure), followed by pig slurry (10<sup>4</sup> gene copies/kg manure). This study shows that the type of manure and spreading equipment can affect the emission rates of airborne bacteria, and ARGs.https://www.mdpi.com/2076-2607/11/7/1797bioaerosolsantimicrobial resistance genesmanure spreading |
| spellingShingle | Mahsa Baghdadi Patrick Brassard Stéphane Godbout Valérie Létourneau Nathalie Turgeon Florent Rossi Émie Lachance Marc Veillette Marie-Lou Gaucher Caroline Duchaine Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ Emission Microorganisms bioaerosols antimicrobial resistance genes manure spreading |
| title | Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ Emission |
| title_full | Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ Emission |
| title_fullStr | Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ Emission |
| title_full_unstemmed | Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ Emission |
| title_short | Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes’ Emission |
| title_sort | contribution of manure spreading operations to bioaerosols and antibiotic resistance genes emission |
| topic | bioaerosols antimicrobial resistance genes manure spreading |
| url | https://www.mdpi.com/2076-2607/11/7/1797 |
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