Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes
The aim of this study was to make the first report on canine heartworm disease in the state of Rondônia and confirm its transmission in this state. Blood samples were randomly collected from 727 dogs in the city of Porto Velho. The samples were analyzed to search for microfilariae and circulating an...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000400559&lng=en&tlng=en |
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| author | Guilherme Maerschner Ogawa Edson Neves da Cruz Príscila Nayara Araújo Cunha Luís Marcelo Aranha Camargo |
| author_facet | Guilherme Maerschner Ogawa Edson Neves da Cruz Príscila Nayara Araújo Cunha Luís Marcelo Aranha Camargo |
| author_sort | Guilherme Maerschner Ogawa |
| collection | DOAJ |
| description | The aim of this study was to make the first report on canine heartworm disease in the state of Rondônia and confirm its transmission in this state. Blood samples were randomly collected from 727 dogs in the city of Porto Velho. The samples were analyzed to search for microfilariae and circulating antigens, using three different techniques: optical microscopy on thick blood smears stained with Giemsa; immunochromatography; and PCR. Mosquitoes were collected inside and outside the homes of all the cases of positive dogs and were tested using PCR to search for DNA of Dirofilaria immitis. Ninety-three blood samples out of 727 (12.8%) were positive according to the immunoassay technique and none according to the thick smear method. Among the 93 positive dogs, 89 (95.7%) were born in Porto Velho. No difference in the frequency of infection was observed between dogs raised indoors and in the yard. PCR on the mosquitoes resulted in only one positive pool. This result shows that the transmission of canine heartworm disease is occurring in the city of Porto Velho and that there is moderate prevalence among the dogs. The techniques of immunochromatography and PCR were more effective for detecting canine heartworm than thick blood smears. The confirmation of canine heartworm disease transmission in Porto Velho places this disease in the ranking for differential diagnosis of pulmonary nodules in humans in Rondônia. |
| first_indexed | 2024-04-12T14:42:33Z |
| format | Article |
| id | doaj.art-69923e54e9624269b231d62680a4c123 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-04-12T14:42:33Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-69923e54e9624269b231d62680a4c1232022-12-22T03:28:46ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296122455956410.1590/S1984-29612013000400017S1984-29612013000400559Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoesGuilherme Maerschner OgawaEdson Neves da CruzPríscila Nayara Araújo CunhaLuís Marcelo Aranha CamargoThe aim of this study was to make the first report on canine heartworm disease in the state of Rondônia and confirm its transmission in this state. Blood samples were randomly collected from 727 dogs in the city of Porto Velho. The samples were analyzed to search for microfilariae and circulating antigens, using three different techniques: optical microscopy on thick blood smears stained with Giemsa; immunochromatography; and PCR. Mosquitoes were collected inside and outside the homes of all the cases of positive dogs and were tested using PCR to search for DNA of Dirofilaria immitis. Ninety-three blood samples out of 727 (12.8%) were positive according to the immunoassay technique and none according to the thick smear method. Among the 93 positive dogs, 89 (95.7%) were born in Porto Velho. No difference in the frequency of infection was observed between dogs raised indoors and in the yard. PCR on the mosquitoes resulted in only one positive pool. This result shows that the transmission of canine heartworm disease is occurring in the city of Porto Velho and that there is moderate prevalence among the dogs. The techniques of immunochromatography and PCR were more effective for detecting canine heartworm than thick blood smears. The confirmation of canine heartworm disease transmission in Porto Velho places this disease in the ranking for differential diagnosis of pulmonary nodules in humans in Rondônia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000400559&lng=en&tlng=enCanine heartworm diseaseDirofilaria immitisPCRimmunochromatographythick blood smearsBrazil |
| spellingShingle | Guilherme Maerschner Ogawa Edson Neves da Cruz Príscila Nayara Araújo Cunha Luís Marcelo Aranha Camargo Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes Revista Brasileira de Parasitologia Veterinária Canine heartworm disease Dirofilaria immitis PCR immunochromatography thick blood smears Brazil |
| title | Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes |
| title_full | Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes |
| title_fullStr | Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes |
| title_full_unstemmed | Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes |
| title_short | Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes |
| title_sort | canine heartworm disease in porto velho first record distribution map and occurrence of positive mosquitoes |
| topic | Canine heartworm disease Dirofilaria immitis PCR immunochromatography thick blood smears Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000400559&lng=en&tlng=en |
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