Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditions
<p>Abstract</p> <p>Background</p> <p>To better understand effects of iron restriction on <it>Actinobacillus pleuropneumoniae </it>and to identify new potential vaccine targets, we conducted transcript profiling studies using a DNA microarray containing all 2...
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| Format: | Article |
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BMC
2007-03-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/72 |
| _version_ | 1811260040765505536 |
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| author | Harel Josée Nash John HE Deslandes Vincent Coulton James W Jacques Mario |
| author_facet | Harel Josée Nash John HE Deslandes Vincent Coulton James W Jacques Mario |
| author_sort | Harel Josée |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>To better understand effects of iron restriction on <it>Actinobacillus pleuropneumoniae </it>and to identify new potential vaccine targets, we conducted transcript profiling studies using a DNA microarray containing all 2025 ORFs of the genome of <it>A. pleuropneumoniae </it>serotype 5b strain L20. This is the first study involving the use of microarray technology to monitor the transcriptome of <it>A. pleuropneumoniae </it>grown under iron restriction.</p> <p>Results</p> <p>Upon comparing growth of this pathogen in iron-sufficient <it>versus </it>iron-depleted medium, 210 genes were identified as being differentially expressed. Some genes (92) were identified as being up-regulated; many have confirmed or putative roles in iron acquisition, such as the genes coding for two TonB energy-transducing proteins and the hemoglobin receptor HgbA. Transcript profiling also led to identification of some new iron acquisition systems of <it>A. pleuropneumoniae</it>. Genes coding for a possible Yfe system (<it>yfeABCD</it>), implicated in the acquisition of chelated iron, were detected, as well as genes coding for a putative enterobactin-type siderophore receptor system. ORFs for homologs of the HmbR system of <it>Neisseria meningitidis </it>involved in iron acquisition from hemoglobin were significantly up-regulated. Down-regulated genes included many that encode proteins containing Fe-S clusters or that use heme as a cofactor. Supplementation of the culture medium with exogenous iron re-established the expression level of these genes.</p> <p>Conclusion</p> <p>We have used transcriptional profiling to generate a list of genes showing differential expression during iron restriction. This strategy enabled us to gain a better understanding of the metabolic changes occurring in response to this stress. Many new potential iron acquisition systems were identified, and further studies will have to be conducted to establish their role during iron restriction.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T18:41:31Z |
| publishDate | 2007-03-01 |
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| series | BMC Genomics |
| spelling | doaj.art-69933904d67746e89b1937b22dbfee4e2022-12-22T03:20:46ZengBMCBMC Genomics1471-21642007-03-01817210.1186/1471-2164-8-72Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditionsHarel JoséeNash John HEDeslandes VincentCoulton James WJacques Mario<p>Abstract</p> <p>Background</p> <p>To better understand effects of iron restriction on <it>Actinobacillus pleuropneumoniae </it>and to identify new potential vaccine targets, we conducted transcript profiling studies using a DNA microarray containing all 2025 ORFs of the genome of <it>A. pleuropneumoniae </it>serotype 5b strain L20. This is the first study involving the use of microarray technology to monitor the transcriptome of <it>A. pleuropneumoniae </it>grown under iron restriction.</p> <p>Results</p> <p>Upon comparing growth of this pathogen in iron-sufficient <it>versus </it>iron-depleted medium, 210 genes were identified as being differentially expressed. Some genes (92) were identified as being up-regulated; many have confirmed or putative roles in iron acquisition, such as the genes coding for two TonB energy-transducing proteins and the hemoglobin receptor HgbA. Transcript profiling also led to identification of some new iron acquisition systems of <it>A. pleuropneumoniae</it>. Genes coding for a possible Yfe system (<it>yfeABCD</it>), implicated in the acquisition of chelated iron, were detected, as well as genes coding for a putative enterobactin-type siderophore receptor system. ORFs for homologs of the HmbR system of <it>Neisseria meningitidis </it>involved in iron acquisition from hemoglobin were significantly up-regulated. Down-regulated genes included many that encode proteins containing Fe-S clusters or that use heme as a cofactor. Supplementation of the culture medium with exogenous iron re-established the expression level of these genes.</p> <p>Conclusion</p> <p>We have used transcriptional profiling to generate a list of genes showing differential expression during iron restriction. This strategy enabled us to gain a better understanding of the metabolic changes occurring in response to this stress. Many new potential iron acquisition systems were identified, and further studies will have to be conducted to establish their role during iron restriction.</p>http://www.biomedcentral.com/1471-2164/8/72 |
| spellingShingle | Harel Josée Nash John HE Deslandes Vincent Coulton James W Jacques Mario Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditions BMC Genomics |
| title | Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditions |
| title_full | Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditions |
| title_fullStr | Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditions |
| title_full_unstemmed | Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditions |
| title_short | Transcriptional profiling of <it>Actinobacillus pleuropneumoniae </it>under iron-restricted conditions |
| title_sort | transcriptional profiling of it actinobacillus pleuropneumoniae it under iron restricted conditions |
| url | http://www.biomedcentral.com/1471-2164/8/72 |
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