Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing Cells
Background: Curcumin (Cur) shows anti-inflammatory and antioxidant effects on central nervous system diseases. The aim of this study was to develop Cur-loaded polymeric and lipid nanoparticles for intranasal delivery to enhance its stability and increase antioxidant effect on olfactory ensheathing c...
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MDPI AG
2021-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/1/159 |
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| author | Angela Bonaccorso Rosalia Pellitteri Barbara Ruozi Carmelo Puglia Debora Santonocito Rosario Pignatello Teresa Musumeci |
| author_facet | Angela Bonaccorso Rosalia Pellitteri Barbara Ruozi Carmelo Puglia Debora Santonocito Rosario Pignatello Teresa Musumeci |
| author_sort | Angela Bonaccorso |
| collection | DOAJ |
| description | Background: Curcumin (Cur) shows anti-inflammatory and antioxidant effects on central nervous system diseases. The aim of this study was to develop Cur-loaded polymeric and lipid nanoparticles for intranasal delivery to enhance its stability and increase antioxidant effect on olfactory ensheathing cells (OECs). Methods: The nanosuspensions were subjected to physico-chemical and technological evaluation through photon correlation spectroscopy (PCS), differential scanning calorimetry (DSC) and UV-spectrophotometry. The cytotoxicity studies of nanosuspensions were carried out on OECs. A viability test was performed after 24 h of exposure of OECs to unloaded and curcumin-loaded nanosuspensions. The potential protective effect of Cur was assessed on hypoxic OECs cells. Uptake studies were performed on the same cell cultures. Thermal analysis was performed to evaluate potential interaction of Cur with a 1,2-Dimyristoyl-<i>sn</i>-glycero-3-phosphocholine (DMPC) biomembrane model. Results: PCS analysis indicated that lipid and polymeric nanosuspensions showed a mean size of 127.10 and 338.20 nm, respectively, high homogeneity and negative zeta potential. Incorporation of Cur into both nanocarriers increased drug stability up to 135 days in cryoprotected freeze-dried nanosuspensions. Cell viability was improved when hypoxic OECs were treated with Cur-loaded polymeric and lipid nanosuspensions compared with the control. Conclusions: Both nanocarriers could improve the stability of Cur as demonstrated by technological studies. Biological studies revealed that both nanocarriers could be used to deliver Cur by intranasal administration for brain targeting. |
| first_indexed | 2024-03-09T05:21:13Z |
| format | Article |
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| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T05:21:13Z |
| publishDate | 2021-01-01 |
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| series | Nanomaterials |
| spelling | doaj.art-6993519e73fc48c68e5bebed3435a8112023-12-03T12:40:20ZengMDPI AGNanomaterials2079-49912021-01-0111115910.3390/nano11010159Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing CellsAngela Bonaccorso0Rosalia Pellitteri1Barbara Ruozi2Carmelo Puglia3Debora Santonocito4Rosario Pignatello5Teresa Musumeci6Department of Drug Sciences, University of Catania, V.le Andrea Doria, 6, 95125 Catania, ItalyInstitute for Biomedical Research and Innovation, National Research Council, Via Paolo Gaifami 18, 95126 Catania, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Drug Sciences, University of Catania, V.le Andrea Doria, 6, 95125 Catania, ItalyDepartment of Drug Sciences, University of Catania, V.le Andrea Doria, 6, 95125 Catania, ItalyDepartment of Drug Sciences, University of Catania, V.le Andrea Doria, 6, 95125 Catania, ItalyDepartment of Drug Sciences, University of Catania, V.le Andrea Doria, 6, 95125 Catania, ItalyBackground: Curcumin (Cur) shows anti-inflammatory and antioxidant effects on central nervous system diseases. The aim of this study was to develop Cur-loaded polymeric and lipid nanoparticles for intranasal delivery to enhance its stability and increase antioxidant effect on olfactory ensheathing cells (OECs). Methods: The nanosuspensions were subjected to physico-chemical and technological evaluation through photon correlation spectroscopy (PCS), differential scanning calorimetry (DSC) and UV-spectrophotometry. The cytotoxicity studies of nanosuspensions were carried out on OECs. A viability test was performed after 24 h of exposure of OECs to unloaded and curcumin-loaded nanosuspensions. The potential protective effect of Cur was assessed on hypoxic OECs cells. Uptake studies were performed on the same cell cultures. Thermal analysis was performed to evaluate potential interaction of Cur with a 1,2-Dimyristoyl-<i>sn</i>-glycero-3-phosphocholine (DMPC) biomembrane model. Results: PCS analysis indicated that lipid and polymeric nanosuspensions showed a mean size of 127.10 and 338.20 nm, respectively, high homogeneity and negative zeta potential. Incorporation of Cur into both nanocarriers increased drug stability up to 135 days in cryoprotected freeze-dried nanosuspensions. Cell viability was improved when hypoxic OECs were treated with Cur-loaded polymeric and lipid nanosuspensions compared with the control. Conclusions: Both nanocarriers could improve the stability of Cur as demonstrated by technological studies. Biological studies revealed that both nanocarriers could be used to deliver Cur by intranasal administration for brain targeting.https://www.mdpi.com/2079-4991/11/1/159intranasal deliverynanomedicineolfactory ensheathing cellscurcuminPLGA |
| spellingShingle | Angela Bonaccorso Rosalia Pellitteri Barbara Ruozi Carmelo Puglia Debora Santonocito Rosario Pignatello Teresa Musumeci Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing Cells Nanomaterials intranasal delivery nanomedicine olfactory ensheathing cells curcumin PLGA |
| title | Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing Cells |
| title_full | Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing Cells |
| title_fullStr | Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing Cells |
| title_full_unstemmed | Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing Cells |
| title_short | Curcumin Loaded Polymeric vs. Lipid Nanoparticles: Antioxidant Effect on Normal and Hypoxic Olfactory Ensheathing Cells |
| title_sort | curcumin loaded polymeric vs lipid nanoparticles antioxidant effect on normal and hypoxic olfactory ensheathing cells |
| topic | intranasal delivery nanomedicine olfactory ensheathing cells curcumin PLGA |
| url | https://www.mdpi.com/2079-4991/11/1/159 |
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