Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation
Fatigue is a common physiological phenomenon caused by many complicated factors. Excessive fatigue will lead to a series of uncomfortable reactions and damage body health. Panax notoginseng leaves (PNL) is a new resource food that good for soothing nerves, nourishing the heart, and strengthening the...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-02-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.824525/full |
| _version_ | 1819280544102350848 |
|---|---|
| author | Min Yang Min Yang Liang Tao Liang Tao Liang Tao Cun-Chao Zhao Cun-Chao Zhao Zi-Lin Wang Zi-Lin Wang Zhi-Jin Yu Zhi-Jin Yu Wen Zhou Yan-Long Wen Yan-Long Wen Ling-Fei Li Ling-Fei Li Yang Tian Yang Tian Yang Tian Jun Sheng Jun Sheng |
| author_facet | Min Yang Min Yang Liang Tao Liang Tao Liang Tao Cun-Chao Zhao Cun-Chao Zhao Zi-Lin Wang Zi-Lin Wang Zhi-Jin Yu Zhi-Jin Yu Wen Zhou Yan-Long Wen Yan-Long Wen Ling-Fei Li Ling-Fei Li Yang Tian Yang Tian Yang Tian Jun Sheng Jun Sheng |
| author_sort | Min Yang |
| collection | DOAJ |
| description | Fatigue is a common physiological phenomenon caused by many complicated factors. Excessive fatigue will lead to a series of uncomfortable reactions and damage body health. Panax notoginseng leaves (PNL) is a new resource food that good for soothing nerves, nourishing the heart, and strengthening the spleen. Microbial fermentation could increase the content of bio-ingredients and produce new active ingredients. However, the effect of fermented P. notoginseng leaves (FPNL) on antifatigue and the molecular mechanisms remain to be elucidated. Thus, in this study, we evaluated the antifatigue effect of co-fermented P. notoginseng leaves by Saccharomyces cerevisiae and Bacillus subtilis in-vitro and in-vivo, and its mechanism was further elucidated. The results showed that FPNL exhibited higher saponins, organic phenolic acids content, and antioxidant activity than PNL. FPNL improved ISO-induced H9c2 myocardial cell damage by alleviating apoptosis (modulating Bax and Bcl-2 protein expression) and reducing antioxidant activity in-vitro. Moreover, in-vivo experiment showed that FPNL significantly prolonged the weight-loading swimming time of mice. After gavaged FPNL, the levels of liver glycogen (LG) and serum lactate dehydrogenase (LDH) activity were increased in mice. In contrast, the levels of blood urea nitrogen (BUN), lactate acid, and malondialdehyde (MDA) were decreased. In summary, our results indicated that FPNL showed a good antifatigue effect in-vivo and in-vitro. |
| first_indexed | 2024-12-24T00:45:29Z |
| format | Article |
| id | doaj.art-6993a6b71c9d4f18b2264bfdabbab232 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-24T00:45:29Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-6993a6b71c9d4f18b2264bfdabbab2322022-12-21T17:23:49ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-02-01910.3389/fnut.2022.824525824525Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo EvaluationMin Yang0Min Yang1Liang Tao2Liang Tao3Liang Tao4Cun-Chao Zhao5Cun-Chao Zhao6Zi-Lin Wang7Zi-Lin Wang8Zhi-Jin Yu9Zhi-Jin Yu10Wen Zhou11Yan-Long Wen12Yan-Long Wen13Ling-Fei Li14Ling-Fei Li15Yang Tian16Yang Tian17Yang Tian18Jun Sheng19Jun Sheng20College of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaNational Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaNational Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaNational Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaNational Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Pu-erh Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaFatigue is a common physiological phenomenon caused by many complicated factors. Excessive fatigue will lead to a series of uncomfortable reactions and damage body health. Panax notoginseng leaves (PNL) is a new resource food that good for soothing nerves, nourishing the heart, and strengthening the spleen. Microbial fermentation could increase the content of bio-ingredients and produce new active ingredients. However, the effect of fermented P. notoginseng leaves (FPNL) on antifatigue and the molecular mechanisms remain to be elucidated. Thus, in this study, we evaluated the antifatigue effect of co-fermented P. notoginseng leaves by Saccharomyces cerevisiae and Bacillus subtilis in-vitro and in-vivo, and its mechanism was further elucidated. The results showed that FPNL exhibited higher saponins, organic phenolic acids content, and antioxidant activity than PNL. FPNL improved ISO-induced H9c2 myocardial cell damage by alleviating apoptosis (modulating Bax and Bcl-2 protein expression) and reducing antioxidant activity in-vitro. Moreover, in-vivo experiment showed that FPNL significantly prolonged the weight-loading swimming time of mice. After gavaged FPNL, the levels of liver glycogen (LG) and serum lactate dehydrogenase (LDH) activity were increased in mice. In contrast, the levels of blood urea nitrogen (BUN), lactate acid, and malondialdehyde (MDA) were decreased. In summary, our results indicated that FPNL showed a good antifatigue effect in-vivo and in-vitro.https://www.frontiersin.org/articles/10.3389/fnut.2022.824525/fullPanax notoginseng leavesmicrobialfermentationweight-loading swimmingantifatigueantioxidant |
| spellingShingle | Min Yang Min Yang Liang Tao Liang Tao Liang Tao Cun-Chao Zhao Cun-Chao Zhao Zi-Lin Wang Zi-Lin Wang Zhi-Jin Yu Zhi-Jin Yu Wen Zhou Yan-Long Wen Yan-Long Wen Ling-Fei Li Ling-Fei Li Yang Tian Yang Tian Yang Tian Jun Sheng Jun Sheng Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation Frontiers in Nutrition Panax notoginseng leaves microbial fermentation weight-loading swimming antifatigue antioxidant |
| title | Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation |
| title_full | Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation |
| title_fullStr | Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation |
| title_full_unstemmed | Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation |
| title_short | Antifatigue Effect of Panax Notoginseng Leaves Fermented With Microorganisms: In-vitro and In-vivo Evaluation |
| title_sort | antifatigue effect of panax notoginseng leaves fermented with microorganisms in vitro and in vivo evaluation |
| topic | Panax notoginseng leaves microbial fermentation weight-loading swimming antifatigue antioxidant |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.824525/full |
| work_keys_str_mv | AT minyang antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT minyang antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT liangtao antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT liangtao antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT liangtao antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT cunchaozhao antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT cunchaozhao antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT zilinwang antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT zilinwang antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT zhijinyu antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT zhijinyu antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT wenzhou antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT yanlongwen antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT yanlongwen antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT lingfeili antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT lingfeili antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT yangtian antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT yangtian antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT yangtian antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT junsheng antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation AT junsheng antifatigueeffectofpanaxnotoginsengleavesfermentedwithmicroorganismsinvitroandinvivoevaluation |