The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer
Objectives. We aimed to assess the genetic differences between cases of early-stage tongue cancer that were positive or negative for lymph node metastasis. Methods. In total, 35 cases of tongue cancer with RNA sequencing data were enrolled in this study. The gene expression profile of the following...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Otorhinolaryngology-Head and Neck Surgery
2022-11-01
|
| Series: | Clinical and Experimental Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://e-ceo.org/upload/pdf/ceo-2022-00150.pdf |
| _version_ | 1811209487035400192 |
|---|---|
| author | Doh Young Lee J. Hun Hah Woo-Jin Jeong Eun-Jae Chung Tack-Kyun Kwon Soon-Hyun Ahn Myung-Whun Sung Seong Keun Kwon |
| author_facet | Doh Young Lee J. Hun Hah Woo-Jin Jeong Eun-Jae Chung Tack-Kyun Kwon Soon-Hyun Ahn Myung-Whun Sung Seong Keun Kwon |
| author_sort | Doh Young Lee |
| collection | DOAJ |
| description | Objectives. We aimed to assess the genetic differences between cases of early-stage tongue cancer that were positive or negative for lymph node metastasis. Methods. In total, 35 cases of tongue cancer with RNA sequencing data were enrolled in this study. The gene expression profile of the following two groups was compared: N0 group (T stage 1 or 2 with N0 stage) and N+ group (T stage 1 or 2 with N+ stage). Using the R and limma packages in the Bioconductor program, we extracted the differentially expressed genes (DEGs). Gene ontology and pathway enrichment analysis were performed using the Database for Annotation, Visualization and Integration Discovery (DAVID) online tool. Immune cell infiltration was analyzed using the CIBERSORT online program. Immunochemical staining of the cancer tissue was evaluated and The Cancer Genome Atlas (TCGA) data were analyzed to validate the identified DEGs. Results. No significant differences were found in the infiltration of 22 types of immune cells. Among a total of 51 identified DEGs, 14 genes were significantly upregulated, while 37 genes were significantly downregulated (P<0.01; fold change >2). Pathway analysis revealed significant associations with the arachidonic acid metabolism-related pathway, calcium signaling, and the muscle contraction pathway. The following DEGs were the most significantly different between the two groups: DEFB4A, SPRR2B, DEFB103B, SPRR2G, DEFB4B, and FAM25A. TCGA data showed that DEFB4A and DEFB103B were more highly expressed in the N0 group than in the N+ group, although the difference did not achieve statistical significance. Immunochemical staining of cancer tissue revealed significantly higher expression of defensin in the N0 group. Conclusions. Defensin (DEFB4A, DEFB103B, DEFB4B) may be a novel biomarker for early regional metastasis in T1/2 tongue cancer. |
| first_indexed | 2024-04-12T04:38:56Z |
| format | Article |
| id | doaj.art-6994b2cc6cd4451a8793b77b11f23133 |
| institution | Directory Open Access Journal |
| issn | 1976-8710 2005-0720 |
| language | English |
| last_indexed | 2024-04-12T04:38:56Z |
| publishDate | 2022-11-01 |
| publisher | Korean Society of Otorhinolaryngology-Head and Neck Surgery |
| record_format | Article |
| series | Clinical and Experimental Otorhinolaryngology |
| spelling | doaj.art-6994b2cc6cd4451a8793b77b11f231332022-12-22T03:47:42ZengKorean Society of Otorhinolaryngology-Head and Neck SurgeryClinical and Experimental Otorhinolaryngology1976-87102005-07202022-11-0115437237910.21053/ceo.2022.00150835The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue CancerDoh Young Lee0J. Hun Hah1Woo-Jin Jeong2Eun-Jae Chung3Tack-Kyun Kwon4Soon-Hyun Ahn5Myung-Whun Sung6Seong Keun Kwon7 Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Boramae Medical Center, Seoul, Korea Department of Otorhinolaryngology-Head and Neck Surgery, THANQ Seoul Thyroid-Head and Neck Surgery Center, Seoul, Korea Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seongnam, Korea Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Korea Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Boramae Medical Center, Seoul, Korea Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Korea Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Korea Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, KoreaObjectives. We aimed to assess the genetic differences between cases of early-stage tongue cancer that were positive or negative for lymph node metastasis. Methods. In total, 35 cases of tongue cancer with RNA sequencing data were enrolled in this study. The gene expression profile of the following two groups was compared: N0 group (T stage 1 or 2 with N0 stage) and N+ group (T stage 1 or 2 with N+ stage). Using the R and limma packages in the Bioconductor program, we extracted the differentially expressed genes (DEGs). Gene ontology and pathway enrichment analysis were performed using the Database for Annotation, Visualization and Integration Discovery (DAVID) online tool. Immune cell infiltration was analyzed using the CIBERSORT online program. Immunochemical staining of the cancer tissue was evaluated and The Cancer Genome Atlas (TCGA) data were analyzed to validate the identified DEGs. Results. No significant differences were found in the infiltration of 22 types of immune cells. Among a total of 51 identified DEGs, 14 genes were significantly upregulated, while 37 genes were significantly downregulated (P<0.01; fold change >2). Pathway analysis revealed significant associations with the arachidonic acid metabolism-related pathway, calcium signaling, and the muscle contraction pathway. The following DEGs were the most significantly different between the two groups: DEFB4A, SPRR2B, DEFB103B, SPRR2G, DEFB4B, and FAM25A. TCGA data showed that DEFB4A and DEFB103B were more highly expressed in the N0 group than in the N+ group, although the difference did not achieve statistical significance. Immunochemical staining of cancer tissue revealed significantly higher expression of defensin in the N0 group. Conclusions. Defensin (DEFB4A, DEFB103B, DEFB4B) may be a novel biomarker for early regional metastasis in T1/2 tongue cancer.http://e-ceo.org/upload/pdf/ceo-2022-00150.pdftongue cancerssquamous cell carcinomametastasisdefensins |
| spellingShingle | Doh Young Lee J. Hun Hah Woo-Jin Jeong Eun-Jae Chung Tack-Kyun Kwon Soon-Hyun Ahn Myung-Whun Sung Seong Keun Kwon The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer Clinical and Experimental Otorhinolaryngology tongue cancers squamous cell carcinoma metastasis defensins |
| title | The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer |
| title_full | The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer |
| title_fullStr | The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer |
| title_full_unstemmed | The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer |
| title_short | The Expression of Defensin-Associated Genes May Be Correlated With Lymph Node Metastasis of Early-Stage Tongue Cancer |
| title_sort | expression of defensin associated genes may be correlated with lymph node metastasis of early stage tongue cancer |
| topic | tongue cancers squamous cell carcinoma metastasis defensins |
| url | http://e-ceo.org/upload/pdf/ceo-2022-00150.pdf |
| work_keys_str_mv | AT dohyounglee theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT jhunhah theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT woojinjeong theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT eunjaechung theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT tackkyunkwon theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT soonhyunahn theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT myungwhunsung theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT seongkeunkwon theexpressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT dohyounglee expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT jhunhah expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT woojinjeong expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT eunjaechung expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT tackkyunkwon expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT soonhyunahn expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT myungwhunsung expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer AT seongkeunkwon expressionofdefensinassociatedgenesmaybecorrelatedwithlymphnodemetastasisofearlystagetonguecancer |