The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells
Porphyra haitanensis, with its rich nutritional value and distinctive flavour, has become a research hotspot in food field. P. haitanensis oligosaccharides (PHO) are oligosaccharides with potential anti-inflammatory properties that ameliorate lipopolysaccharide-induced inflammatory responses in the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624000070 |
| _version_ | 1797356126160814080 |
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| author | Si-Min Qiu Suresh Veeraperumal Karsoon Tan Saiyi Zhong Kit-Leong Cheong |
| author_facet | Si-Min Qiu Suresh Veeraperumal Karsoon Tan Saiyi Zhong Kit-Leong Cheong |
| author_sort | Si-Min Qiu |
| collection | DOAJ |
| description | Porphyra haitanensis, with its rich nutritional value and distinctive flavour, has become a research hotspot in food field. P. haitanensis oligosaccharides (PHO) are oligosaccharides with potential anti-inflammatory properties that ameliorate lipopolysaccharide-induced inflammatory responses in the in vitro IEC-6 cell line model. Here, the structure of PHO was investigated, and LPS–induced injury mechanisms in IEC-6 were determined by cellular, molecular, and transcriptome analyses. After PHO exposure, LPS-induced IEC-6 cells showed reduced apoptosis, decreased oxidative stress levels, improved cell membrane integrity, and paracellular permeability. This study shows that PHO attenuates injury in the IEC-6 cell line by increasing the expression of tight junctions. PHO also inhibited the TLR4/NF-κB pathway and caused downregulation of intracellular pro-inflammatory cytokines, which attenuates LPS-induced injury in an in vitro model, and further offers the possibility of restoring the intestinal barrier. This study provides insight into the role of PHO in alleviating the intestinal epithelial barrier damage. |
| first_indexed | 2024-03-08T14:21:07Z |
| format | Article |
| id | doaj.art-6996ef8522f84fa78ac12e93b1702449 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-08T14:21:07Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6996ef8522f84fa78ac12e93b17024492024-01-14T05:37:32ZengElsevierJournal of Functional Foods1756-46462024-01-01112106005The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cellsSi-Min Qiu0Suresh Veeraperumal1Karsoon Tan2Saiyi Zhong3Kit-Leong Cheong4Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, ChinaDepartment of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, ChinaGuangxi Key Laboratory of Beibu Gulf Biodiversity Conservation, Beibu Gulf University, Qinzhou, Guangxi, China; Corresponding authors.Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; Corresponding authors.Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, China; Corresponding authors.Porphyra haitanensis, with its rich nutritional value and distinctive flavour, has become a research hotspot in food field. P. haitanensis oligosaccharides (PHO) are oligosaccharides with potential anti-inflammatory properties that ameliorate lipopolysaccharide-induced inflammatory responses in the in vitro IEC-6 cell line model. Here, the structure of PHO was investigated, and LPS–induced injury mechanisms in IEC-6 were determined by cellular, molecular, and transcriptome analyses. After PHO exposure, LPS-induced IEC-6 cells showed reduced apoptosis, decreased oxidative stress levels, improved cell membrane integrity, and paracellular permeability. This study shows that PHO attenuates injury in the IEC-6 cell line by increasing the expression of tight junctions. PHO also inhibited the TLR4/NF-κB pathway and caused downregulation of intracellular pro-inflammatory cytokines, which attenuates LPS-induced injury in an in vitro model, and further offers the possibility of restoring the intestinal barrier. This study provides insight into the role of PHO in alleviating the intestinal epithelial barrier damage.http://www.sciencedirect.com/science/article/pii/S1756464624000070Porphyra haitanensis oligosaccharidesTight junctionsIntestinal epithelial barrierTLR4/NF-κB pathway |
| spellingShingle | Si-Min Qiu Suresh Veeraperumal Karsoon Tan Saiyi Zhong Kit-Leong Cheong The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells Journal of Functional Foods Porphyra haitanensis oligosaccharides Tight junctions Intestinal epithelial barrier TLR4/NF-κB pathway |
| title | The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells |
| title_full | The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells |
| title_fullStr | The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells |
| title_full_unstemmed | The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells |
| title_short | The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells |
| title_sort | in vitro anti inflammatory mechanism of porphyra haitanensis oligosaccharides on lipopolysaccharide induced injury in iec 6 cells |
| topic | Porphyra haitanensis oligosaccharides Tight junctions Intestinal epithelial barrier TLR4/NF-κB pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624000070 |
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