A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice
Bioluminescence imaging of neuronal activity is desired. Here the authors report a luciferase prosubstrate activatable in vivo by nonspecific esterase and engineer a bioluminescent indicator with responsiveness to calcium ions: integration of these components enabled imaging of neuronal activity in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-31673-x |
| _version_ | 1811324377148424192 |
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| author | Xiaodong Tian Yiyu Zhang Xinyu Li Ying Xiong Tianchen Wu Hui-Wang Ai |
| author_facet | Xiaodong Tian Yiyu Zhang Xinyu Li Ying Xiong Tianchen Wu Hui-Wang Ai |
| author_sort | Xiaodong Tian |
| collection | DOAJ |
| description | Bioluminescence imaging of neuronal activity is desired. Here the authors report a luciferase prosubstrate activatable in vivo by nonspecific esterase and engineer a bioluminescent indicator with responsiveness to calcium ions: integration of these components enabled imaging of neuronal activity in mice. |
| first_indexed | 2024-04-13T14:13:10Z |
| format | Article |
| id | doaj.art-699731f9e86b45eaa8e2a839bd191d47 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T14:13:10Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-699731f9e86b45eaa8e2a839bd191d472022-12-22T02:43:44ZengNature PortfolioNature Communications2041-17232022-07-0113111310.1038/s41467-022-31673-xA luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in miceXiaodong Tian0Yiyu Zhang1Xinyu Li2Ying Xiong3Tianchen Wu4Hui-Wang Ai5Department of Molecular Physiology and Biological Physics, University of VirginiaDepartment of Molecular Physiology and Biological Physics, University of VirginiaDepartment of Molecular Physiology and Biological Physics, University of VirginiaDepartment of Molecular Physiology and Biological Physics, University of VirginiaDepartment of Molecular Physiology and Biological Physics, University of VirginiaDepartment of Molecular Physiology and Biological Physics, University of VirginiaBioluminescence imaging of neuronal activity is desired. Here the authors report a luciferase prosubstrate activatable in vivo by nonspecific esterase and engineer a bioluminescent indicator with responsiveness to calcium ions: integration of these components enabled imaging of neuronal activity in mice.https://doi.org/10.1038/s41467-022-31673-x |
| spellingShingle | Xiaodong Tian Yiyu Zhang Xinyu Li Ying Xiong Tianchen Wu Hui-Wang Ai A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice Nature Communications |
| title | A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice |
| title_full | A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice |
| title_fullStr | A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice |
| title_full_unstemmed | A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice |
| title_short | A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice |
| title_sort | luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice |
| url | https://doi.org/10.1038/s41467-022-31673-x |
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