Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian population
Abstract There is no valid instrument to assess n-3 polyunsaturated fatty acids (n-3 PUFAs) intake in Iran. This study aims to develop a food frequency questionnaire (FFQ) that estimates the intake of n-3 PUFA and validate it in a healthy Iranian population based on the n-3 PUFA content of red blood...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41623-2 |
| _version_ | 1827634392621645824 |
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| author | Nasim Abedimanesh Behrooz Motlagh Jalal Hejazi Mohammad Reza Eskandari Mohammad Asghari-Jafarabadi Saeideh Mazloomzadeh |
| author_facet | Nasim Abedimanesh Behrooz Motlagh Jalal Hejazi Mohammad Reza Eskandari Mohammad Asghari-Jafarabadi Saeideh Mazloomzadeh |
| author_sort | Nasim Abedimanesh |
| collection | DOAJ |
| description | Abstract There is no valid instrument to assess n-3 polyunsaturated fatty acids (n-3 PUFAs) intake in Iran. This study aims to develop a food frequency questionnaire (FFQ) that estimates the intake of n-3 PUFA and validate it in a healthy Iranian population based on the n-3 PUFA content of red blood cells (RBCs) and a 3-day food record (FR). A healthy population (n = 221) was recruited between February and July 2021. Participants completed the new FFQ and 3-day FR to evaluate the average intake of n-3 PUFAs. We used gas chromatography to assess the n-3 PUFA content of RBCs. To validate the FFQ based on FR and biomarker as references, the correlation coefficient was calculated. According to the Bland–Altman plots, a good agreement was found between the new FFQ and FR. Moreover, absolute intake values of ALA, EPA, DPA, DHA, and total n-3 PUFAs based on FFQ were positively correlated to their respective RBC membrane levels (coefficients between 0.205 and 0.508, p < 0.005) and FR (coefficients between 0.771 and 0.827, p < 0.001). This new FFQ is a valid instrument that can be applied to estimate the n-3 PUFA status of healthy Iranian adults. |
| first_indexed | 2024-03-09T15:13:39Z |
| format | Article |
| id | doaj.art-699aaae7cfa148609fd12397e6e6ad7b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:13:39Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-699aaae7cfa148609fd12397e6e6ad7b2023-11-26T13:14:13ZengNature PortfolioScientific Reports2045-23222023-09-011311910.1038/s41598-023-41623-2Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian populationNasim Abedimanesh0Behrooz Motlagh1Jalal Hejazi2Mohammad Reza Eskandari3Mohammad Asghari-Jafarabadi4Saeideh Mazloomzadeh5Social Determinants of Health Research Center, Zanjan University of Medical SciencesDepartment of Clinical Biochemistry, School of Medicine, Zanjan University of Medical SciencesDepartment of Nutrition, School of Medicine, Zanjan University of Medical SciencesDepartment of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical SciencesCabrini Research, Cabrini HealthRajaie Cardiovascular, Medical and Research CenterAbstract There is no valid instrument to assess n-3 polyunsaturated fatty acids (n-3 PUFAs) intake in Iran. This study aims to develop a food frequency questionnaire (FFQ) that estimates the intake of n-3 PUFA and validate it in a healthy Iranian population based on the n-3 PUFA content of red blood cells (RBCs) and a 3-day food record (FR). A healthy population (n = 221) was recruited between February and July 2021. Participants completed the new FFQ and 3-day FR to evaluate the average intake of n-3 PUFAs. We used gas chromatography to assess the n-3 PUFA content of RBCs. To validate the FFQ based on FR and biomarker as references, the correlation coefficient was calculated. According to the Bland–Altman plots, a good agreement was found between the new FFQ and FR. Moreover, absolute intake values of ALA, EPA, DPA, DHA, and total n-3 PUFAs based on FFQ were positively correlated to their respective RBC membrane levels (coefficients between 0.205 and 0.508, p < 0.005) and FR (coefficients between 0.771 and 0.827, p < 0.001). This new FFQ is a valid instrument that can be applied to estimate the n-3 PUFA status of healthy Iranian adults.https://doi.org/10.1038/s41598-023-41623-2 |
| spellingShingle | Nasim Abedimanesh Behrooz Motlagh Jalal Hejazi Mohammad Reza Eskandari Mohammad Asghari-Jafarabadi Saeideh Mazloomzadeh Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian population Scientific Reports |
| title | Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian population |
| title_full | Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian population |
| title_fullStr | Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian population |
| title_full_unstemmed | Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian population |
| title_short | Biomarker-based validation of a food frequency questionnaire for the assessment of omega-3 fatty acid status in a healthy Iranian population |
| title_sort | biomarker based validation of a food frequency questionnaire for the assessment of omega 3 fatty acid status in a healthy iranian population |
| url | https://doi.org/10.1038/s41598-023-41623-2 |
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