Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells
The purpose of this study was to determine the antiallergic effects of AF-343, a mixture of natural plant extracts from <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on rat basophilic leukemia (RBL-2H3) cells. The inhibitory effec...
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MDPI AG
2020-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/10/2434 |
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| author | Eun Kyeong Lee Jeongah Song Youjin Seo Eun Mi Koh Seon-Hee Kim Kyung Jin Jung |
| author_facet | Eun Kyeong Lee Jeongah Song Youjin Seo Eun Mi Koh Seon-Hee Kim Kyung Jin Jung |
| author_sort | Eun Kyeong Lee |
| collection | DOAJ |
| description | The purpose of this study was to determine the antiallergic effects of AF-343, a mixture of natural plant extracts from <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on rat basophilic leukemia (RBL-2H3) cells. The inhibitory effects on cell degranulation, proinflammatory cytokine secretion, and reactive oxygen species (ROS) production were studied in compound 48/80-treated RBL-2H3 cells. The bioactive compounds in AF-343 were also identified by HPLC–UV. AF-343 was found to effectively suppress compound 48/80-induced β-hexosaminidase release, and interleukin (IL)-4 and tumor necrosis factor-α (TNF-α) production in RBL-2H3 cells. In addition, AF-343 exhibited DPPH free radical scavenging effects in vitro (half-maximal inhibitory concentration (IC<sub>50</sub>) = 105 μg/mL) and potently inhibited compound 48/80-induced cellular ROS generation in a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) assay. Specifically, treatment with AF-343 exerted stronger antioxidant effects in vitro and antiallergic effects in cells than treatment with three single natural plant extracts. Furthermore, AF-343 was observed to contain bioactive compounds, including catechin, aurantio-obtusin, and chicoric acid, which have been reported to elicit antiallergic responses. This study reveals that AF-343 attenuates allergic responses via suppression of β-hexosaminidase release, IL-4 and TNF-α secretion, and ROS generation, perhaps through mechanisms related to catechin, aurantio-obtusin, and chicoric acid. The results indicate that AF-343 can be considered a treatment for various allergic diseases. |
| first_indexed | 2024-03-10T19:38:39Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:38:39Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-699b256fa532460eb1a7b9990191799e2023-11-20T01:26:39ZengMDPI AGMolecules1420-30492020-05-012510243410.3390/molecules25102434Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 CellsEun Kyeong Lee0Jeongah Song1Youjin Seo2Eun Mi Koh3Seon-Hee Kim4Kyung Jin Jung5Bioanalytical and Immunoanalytical Research Group, Korea Institute of Toxicology, 141 Gajungro, Yuseong-gu, Daejeon 34114, KoreaAnimal Model Research Group, Korea Institute of Toxicology, Jeongeup, Jeonbuk 580-185, KoreaJeonbuk Analytical Research Group, Korea Institute of Toxicology, Jeongeup, Jeonbuk 580-185, KoreaBioanalytical and Immunoanalytical Research Group, Korea Institute of Toxicology, 141 Gajungro, Yuseong-gu, Daejeon 34114, KoreaSungkyun Biotech Co., Ltd., SungKyunKwan University, #2066, Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do 61256, KoreaBioanalytical and Immunoanalytical Research Group, Korea Institute of Toxicology, 141 Gajungro, Yuseong-gu, Daejeon 34114, KoreaThe purpose of this study was to determine the antiallergic effects of AF-343, a mixture of natural plant extracts from <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on rat basophilic leukemia (RBL-2H3) cells. The inhibitory effects on cell degranulation, proinflammatory cytokine secretion, and reactive oxygen species (ROS) production were studied in compound 48/80-treated RBL-2H3 cells. The bioactive compounds in AF-343 were also identified by HPLC–UV. AF-343 was found to effectively suppress compound 48/80-induced β-hexosaminidase release, and interleukin (IL)-4 and tumor necrosis factor-α (TNF-α) production in RBL-2H3 cells. In addition, AF-343 exhibited DPPH free radical scavenging effects in vitro (half-maximal inhibitory concentration (IC<sub>50</sub>) = 105 μg/mL) and potently inhibited compound 48/80-induced cellular ROS generation in a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) assay. Specifically, treatment with AF-343 exerted stronger antioxidant effects in vitro and antiallergic effects in cells than treatment with three single natural plant extracts. Furthermore, AF-343 was observed to contain bioactive compounds, including catechin, aurantio-obtusin, and chicoric acid, which have been reported to elicit antiallergic responses. This study reveals that AF-343 attenuates allergic responses via suppression of β-hexosaminidase release, IL-4 and TNF-α secretion, and ROS generation, perhaps through mechanisms related to catechin, aurantio-obtusin, and chicoric acid. The results indicate that AF-343 can be considered a treatment for various allergic diseases.https://www.mdpi.com/1420-3049/25/10/2434AF-343<i>Cassia tora</i> L., <i>Ulmus pumila</i> L., <i>Taraxacum officinale</i>antiallergic responsescompound 48/80RBL-2H3 cells |
| spellingShingle | Eun Kyeong Lee Jeongah Song Youjin Seo Eun Mi Koh Seon-Hee Kim Kyung Jin Jung Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells Molecules AF-343 <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., <i>Taraxacum officinale</i> antiallergic responses compound 48/80 RBL-2H3 cells |
| title | Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells |
| title_full | Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells |
| title_fullStr | Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells |
| title_full_unstemmed | Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells |
| title_short | Inhibitory Effects of AF-343, a Mixture of <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., and <i>Taraxacum officinale</i>, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells |
| title_sort | inhibitory effects of af 343 a mixture of i cassia tora i l i ulmus pumila i l and i taraxacum officinale i on compound 48 80 mediated allergic responses in rbl 2h3 cells |
| topic | AF-343 <i>Cassia tora</i> L., <i>Ulmus pumila</i> L., <i>Taraxacum officinale</i> antiallergic responses compound 48/80 RBL-2H3 cells |
| url | https://www.mdpi.com/1420-3049/25/10/2434 |
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