Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea)
The biological activity of extract obtained from the ascidians <em>Styela plicata</em> and <em>Ascidia mentula</em>, living in the coastal lake Faro (Messina, Italy) was ascertained with specific regard to cytotoxic effect, tested on cultured human embryonic kidney (HEK 293 P...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2016-06-01
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| Series: | Journal of Biological Research |
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| Online Access: | http://www.pagepressjournals.org/index.php/jbr/article/view/5812 |
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| author | Satheesh Kumar Palanisamy Rossana Morabito Alessia Remigante Nunziacarla Spanò Giuseppina La Spada Salvatore Giacobbe Angela Marino |
| author_facet | Satheesh Kumar Palanisamy Rossana Morabito Alessia Remigante Nunziacarla Spanò Giuseppina La Spada Salvatore Giacobbe Angela Marino |
| author_sort | Satheesh Kumar Palanisamy |
| collection | DOAJ |
| description | The biological activity of extract obtained from the ascidians <em>Styela plicata</em> and <em>Ascidia mentula</em>, living in the coastal lake Faro (Messina, Italy) was ascertained with specific regard to cytotoxic effect, tested on cultured human embryonic kidney (HEK 293 Phoenix) cells after 12, 24 or 48 h, and to antimicrobial effect (10 mg/mL), assayed on both Gram positive and negative strains. Nitrosative stress, possibly induced by the extract on HEK 293 cells and assessed by NO<sub>2</sub> <sup>–</sup>/NO<sub>3</sub> <sup>–</sup> production measurement, was also verified. With regard to cytotoxic activity, <em>A. mentula</em> extract was more effective than <em>S. plicata</em> one, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, with toxic action due to nitrosative stress. <em>S. plicata</em> extract had a more significant antimicrobial activity than <em>A. mentula</em>. The present findings provide a first evidence of the biological power exhibited by both <em>S. plicata</em> and <em>A. mentula</em> extracts, thus increasing the knowledge about the biological activity of marine compounds and providing novel information to possibly correlate the different toxicity pattern displayed by both specimens and their distribution in the lake Faro (Messina). |
| first_indexed | 2024-12-10T22:42:12Z |
| format | Article |
| id | doaj.art-699b55f40d9f4b4eabce20c0c4b14045 |
| institution | Directory Open Access Journal |
| issn | 1826-8838 2284-0230 |
| language | English |
| last_indexed | 2024-12-10T22:42:12Z |
| publishDate | 2016-06-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj.art-699b55f40d9f4b4eabce20c0c4b140452022-12-22T01:30:41ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302016-06-0189110.4081/jbr.2016.58124839Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea)Satheesh Kumar Palanisamy0Rossana Morabito1Alessia Remigante2Nunziacarla Spanò3Giuseppina La Spada4Salvatore Giacobbe5Angela Marino6Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, MessinaDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, MessinaThe biological activity of extract obtained from the ascidians <em>Styela plicata</em> and <em>Ascidia mentula</em>, living in the coastal lake Faro (Messina, Italy) was ascertained with specific regard to cytotoxic effect, tested on cultured human embryonic kidney (HEK 293 Phoenix) cells after 12, 24 or 48 h, and to antimicrobial effect (10 mg/mL), assayed on both Gram positive and negative strains. Nitrosative stress, possibly induced by the extract on HEK 293 cells and assessed by NO<sub>2</sub> <sup>–</sup>/NO<sub>3</sub> <sup>–</sup> production measurement, was also verified. With regard to cytotoxic activity, <em>A. mentula</em> extract was more effective than <em>S. plicata</em> one, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, with toxic action due to nitrosative stress. <em>S. plicata</em> extract had a more significant antimicrobial activity than <em>A. mentula</em>. The present findings provide a first evidence of the biological power exhibited by both <em>S. plicata</em> and <em>A. mentula</em> extracts, thus increasing the knowledge about the biological activity of marine compounds and providing novel information to possibly correlate the different toxicity pattern displayed by both specimens and their distribution in the lake Faro (Messina).http://www.pagepressjournals.org/index.php/jbr/article/view/5812Styela plicataAscidia mentulaExtractBiological activityAscidians |
| spellingShingle | Satheesh Kumar Palanisamy Rossana Morabito Alessia Remigante Nunziacarla Spanò Giuseppina La Spada Salvatore Giacobbe Angela Marino Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea) Journal of Biological Research Styela plicata Ascidia mentula Extract Biological activity Ascidians |
| title | Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea) |
| title_full | Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea) |
| title_fullStr | Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea) |
| title_full_unstemmed | Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea) |
| title_short | Biological activity of extract from Styela plicata and Ascidia mentula (Ascidiacea) |
| title_sort | biological activity of extract from styela plicata and ascidia mentula ascidiacea |
| topic | Styela plicata Ascidia mentula Extract Biological activity Ascidians |
| url | http://www.pagepressjournals.org/index.php/jbr/article/view/5812 |
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