Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based Films
Chitosan-based film with and without antagonistic yeast was prepared and its effect against <i>Penicillium italicum</i> was evaluated. The biocompatibility of yeast cells in the developed films was assessed in terms of population dynamics. Furthermore, the impact on physicochemical prope...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/4/369 |
| _version_ | 1827619647888818176 |
|---|---|
| author | César A. García-Bramasco Francisco J. Blancas-Benitez Beatriz Montaño-Leyva Laura M. Medrano-Castellón Porfirio Gutierrez-Martinez Ramsés R. González-Estrada |
| author_facet | César A. García-Bramasco Francisco J. Blancas-Benitez Beatriz Montaño-Leyva Laura M. Medrano-Castellón Porfirio Gutierrez-Martinez Ramsés R. González-Estrada |
| author_sort | César A. García-Bramasco |
| collection | DOAJ |
| description | Chitosan-based film with and without antagonistic yeast was prepared and its effect against <i>Penicillium italicum</i> was evaluated. The biocompatibility of yeast cells in the developed films was assessed in terms of population dynamics. Furthermore, the impact on physicochemical properties of the prepared films with and without yeast cells incorporated were evaluated in terms of thickness, mechanical properties, color and opacity. Chitosan films with the antagonistic yeast entrapped exhibited strong antifungal activity by inhibiting the mycelial development (55%), germination (45%) and reducing the sporulation process (87%). Chitosan matrix at 0.5% and 1.0% was maintained over 9 days of cell viability. However, at 1.5% of chitosan the population dynamics was strongly affected. The addition of yeast cells only impacted color values such as a*, b*, chroma and hue angle when 1.0% of chitosan concentration was used. Conversely, luminosity was not affected in the presence of yeast cells as well as the opacity. Besides, the addition of antagonistic yeast improved the mechanical resistance of the films. The addition of <i>D. hansenii</i> in chitosan films improve their efficacy for controlling <i>P. italicum,</i> and besides showed desirable characteristics for future use as packaging for citrus products. |
| first_indexed | 2024-03-09T10:33:02Z |
| format | Article |
| id | doaj.art-699ba99cc19944e0b0444e02e47be59a |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T10:33:02Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-699ba99cc19944e0b0444e02e47be59a2023-12-01T21:08:19ZengMDPI AGJournal of Fungi2309-608X2022-04-018436910.3390/jof8040369Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based FilmsCésar A. García-Bramasco0Francisco J. Blancas-Benitez1Beatriz Montaño-Leyva2Laura M. Medrano-Castellón3Porfirio Gutierrez-Martinez4Ramsés R. González-Estrada5Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Avenida Tecnológico 2595, Col. Lagos del Country, Tepic 63175, Nayarit, MexicoTecnológico Nacional de México/Instituto Tecnológico de Tepic, Avenida Tecnológico 2595, Col. Lagos del Country, Tepic 63175, Nayarit, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Rosales y Blvd. Luis Encinas, C.P., Hermosillo 83000, Sonora, MexicoTecnológico Nacional de México/Instituto Tecnológico de Tepic, Avenida Tecnológico 2595, Col. Lagos del Country, Tepic 63175, Nayarit, MexicoTecnológico Nacional de México/Instituto Tecnológico de Tepic, Avenida Tecnológico 2595, Col. Lagos del Country, Tepic 63175, Nayarit, MexicoTecnológico Nacional de México/Instituto Tecnológico de Tepic, Avenida Tecnológico 2595, Col. Lagos del Country, Tepic 63175, Nayarit, MexicoChitosan-based film with and without antagonistic yeast was prepared and its effect against <i>Penicillium italicum</i> was evaluated. The biocompatibility of yeast cells in the developed films was assessed in terms of population dynamics. Furthermore, the impact on physicochemical properties of the prepared films with and without yeast cells incorporated were evaluated in terms of thickness, mechanical properties, color and opacity. Chitosan films with the antagonistic yeast entrapped exhibited strong antifungal activity by inhibiting the mycelial development (55%), germination (45%) and reducing the sporulation process (87%). Chitosan matrix at 0.5% and 1.0% was maintained over 9 days of cell viability. However, at 1.5% of chitosan the population dynamics was strongly affected. The addition of yeast cells only impacted color values such as a*, b*, chroma and hue angle when 1.0% of chitosan concentration was used. Conversely, luminosity was not affected in the presence of yeast cells as well as the opacity. Besides, the addition of antagonistic yeast improved the mechanical resistance of the films. The addition of <i>D. hansenii</i> in chitosan films improve their efficacy for controlling <i>P. italicum,</i> and besides showed desirable characteristics for future use as packaging for citrus products.https://www.mdpi.com/2309-608X/8/4/369biocontrol potentialcell viabilitychitosan films<i>Penicillium italicum</i> |
| spellingShingle | César A. García-Bramasco Francisco J. Blancas-Benitez Beatriz Montaño-Leyva Laura M. Medrano-Castellón Porfirio Gutierrez-Martinez Ramsés R. González-Estrada Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based Films Journal of Fungi biocontrol potential cell viability chitosan films <i>Penicillium italicum</i> |
| title | Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based Films |
| title_full | Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based Films |
| title_fullStr | Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based Films |
| title_full_unstemmed | Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based Films |
| title_short | Influence of Marine Yeast <i>Debaryomyces hansenii</i> on Antifungal and Physicochemical Properties of Chitosan-Based Films |
| title_sort | influence of marine yeast i debaryomyces hansenii i on antifungal and physicochemical properties of chitosan based films |
| topic | biocontrol potential cell viability chitosan films <i>Penicillium italicum</i> |
| url | https://www.mdpi.com/2309-608X/8/4/369 |
| work_keys_str_mv | AT cesaragarciabramasco influenceofmarineyeastidebaryomyceshanseniiionantifungalandphysicochemicalpropertiesofchitosanbasedfilms AT franciscojblancasbenitez influenceofmarineyeastidebaryomyceshanseniiionantifungalandphysicochemicalpropertiesofchitosanbasedfilms AT beatrizmontanoleyva influenceofmarineyeastidebaryomyceshanseniiionantifungalandphysicochemicalpropertiesofchitosanbasedfilms AT laurammedranocastellon influenceofmarineyeastidebaryomyceshanseniiionantifungalandphysicochemicalpropertiesofchitosanbasedfilms AT porfiriogutierrezmartinez influenceofmarineyeastidebaryomyceshanseniiionantifungalandphysicochemicalpropertiesofchitosanbasedfilms AT ramsesrgonzalezestrada influenceofmarineyeastidebaryomyceshanseniiionantifungalandphysicochemicalpropertiesofchitosanbasedfilms |