Synergistic antifungal effect of glabridin and fluconazole.
The incidence of invasive fungal infections is increasing in recent years. The present study mainly investigated glabridin (Gla) alone and especially in combination with fluconazole (FLC) against Cryptococcus neoformans and Candida species (Candida albicans, Candida tropicalis, Candida krusei, Candi...
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Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4110026?pdf=render |
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author | Wei Liu Li Ping Li Jun Dong Zhang Qun Li Hui Shen Si Min Chen Li Juan He Lan Yan Guo Tong Xu Mao Mao An Yuan Ying Jiang |
author_facet | Wei Liu Li Ping Li Jun Dong Zhang Qun Li Hui Shen Si Min Chen Li Juan He Lan Yan Guo Tong Xu Mao Mao An Yuan Ying Jiang |
author_sort | Wei Liu |
collection | DOAJ |
description | The incidence of invasive fungal infections is increasing in recent years. The present study mainly investigated glabridin (Gla) alone and especially in combination with fluconazole (FLC) against Cryptococcus neoformans and Candida species (Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis and Candida Glabratas) by different methods. The minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) indicated that Gla possessed a broad-spectrum antifungal activity at relatively high concentrations. After combining with FLC, Gla exerted a potent synergistic effect against drug-resistant C. albicans and C. tropicalis at lower concentrations when interpreted by fractional inhibitory concentration index (FICI). Disk diffusion test and time-killing test confirming the synergistic fungicidal effect. Cell growth tests suggested that the synergistic effect of the two drugs depended more on the concentration of Gla. The cell envelop damage including a significant decrease of cell size and membrane permeability increasing were found after Gla treatment. Together, our results suggested that Gla possessed a synergistic effect with FLC and the cell envelope damage maybe contributed to the synergistic effect, which providing new information for developing novel antifungal agents. |
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institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
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spelling | doaj.art-699d1da1a6cf4468b9c11d137826e84b2022-12-22T03:34:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10344210.1371/journal.pone.0103442Synergistic antifungal effect of glabridin and fluconazole.Wei LiuLi Ping LiJun Dong ZhangQun LiHui ShenSi Min ChenLi Juan HeLan YanGuo Tong XuMao Mao AnYuan Ying JiangThe incidence of invasive fungal infections is increasing in recent years. The present study mainly investigated glabridin (Gla) alone and especially in combination with fluconazole (FLC) against Cryptococcus neoformans and Candida species (Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis and Candida Glabratas) by different methods. The minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) indicated that Gla possessed a broad-spectrum antifungal activity at relatively high concentrations. After combining with FLC, Gla exerted a potent synergistic effect against drug-resistant C. albicans and C. tropicalis at lower concentrations when interpreted by fractional inhibitory concentration index (FICI). Disk diffusion test and time-killing test confirming the synergistic fungicidal effect. Cell growth tests suggested that the synergistic effect of the two drugs depended more on the concentration of Gla. The cell envelop damage including a significant decrease of cell size and membrane permeability increasing were found after Gla treatment. Together, our results suggested that Gla possessed a synergistic effect with FLC and the cell envelope damage maybe contributed to the synergistic effect, which providing new information for developing novel antifungal agents.http://europepmc.org/articles/PMC4110026?pdf=render |
spellingShingle | Wei Liu Li Ping Li Jun Dong Zhang Qun Li Hui Shen Si Min Chen Li Juan He Lan Yan Guo Tong Xu Mao Mao An Yuan Ying Jiang Synergistic antifungal effect of glabridin and fluconazole. PLoS ONE |
title | Synergistic antifungal effect of glabridin and fluconazole. |
title_full | Synergistic antifungal effect of glabridin and fluconazole. |
title_fullStr | Synergistic antifungal effect of glabridin and fluconazole. |
title_full_unstemmed | Synergistic antifungal effect of glabridin and fluconazole. |
title_short | Synergistic antifungal effect of glabridin and fluconazole. |
title_sort | synergistic antifungal effect of glabridin and fluconazole |
url | http://europepmc.org/articles/PMC4110026?pdf=render |
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