Development of the Method to Produce Functionally Active Recombinant Streptavidin in Escherichia coli Cells
Streptavidin is a homotetrameric protein produced by Streptomyces avidinii, each subunit of which binds biotin (vitamin H), forming a stable complex (Kd = 10-15 М). Streptavidin-biotin coreaction is widely used in analytical systems, for targeted delivery of compounds, for affinity purification, etc...
Main Authors: | Eugenia E. Bashmakova, Alexander N. Kudryavtsev, Ludmila A. Frank |
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Format: | Article |
Language: | English |
Published: |
Siberian Federal University
2020-06-01
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Series: | Журнал Сибирского федерального университета: Серия Биология |
Subjects: | |
Online Access: | http://elib.sfu-kras.ru/bitstream/2311/135353/1/08_Bashmakova.pdf |
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