Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes
GM3 is implicated in cell signaling, inflammation and insulin resistance. The intestinal mucosa metabolizes ganglioside and provides gangliosides for uptake by peripheral tissues. Gangliosides downregulate acute and chronic inflammatory signals. It is likely that transport of intestinal derived gang...
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MDPI AG
2022-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/12/3141 |
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| author | Irma Magaly Rivas Serna Michelle Beveridge Michaelann Wilke Edmond A. Ryan Michael Thomas Clandinin Vera Christine Mazurak |
| author_facet | Irma Magaly Rivas Serna Michelle Beveridge Michaelann Wilke Edmond A. Ryan Michael Thomas Clandinin Vera Christine Mazurak |
| author_sort | Irma Magaly Rivas Serna |
| collection | DOAJ |
| description | GM3 is implicated in cell signaling, inflammation and insulin resistance. The intestinal mucosa metabolizes ganglioside and provides gangliosides for uptake by peripheral tissues. Gangliosides downregulate acute and chronic inflammatory signals. It is likely that transport of intestinal derived gangliosides to other tissues impact the same signals characteristic of inflammatory change in other chronic conditions such as Type 2 Diabetes (T2DM). The postprandial ceramide composition of GM3 and other gangliosides in plasma and chylomicrons has not been examined in T2DM. The present study assessed if diet or T2DM alters ganglioside components in plasma and chylomicrons secreted from the intestinal mucosa after a meal. GD1, GD3, and GM3 content of chylomicrons and plasma was determined by LC/triple quad MS in non-diabetic (control) and T2DM individuals in the fasting and postprandial state after 2 days of consuming a low or high fat diet in a randomized blinded crossover design. Diet fat level did not alter baseline plasma or chylomicron ganglioside levels. Four hours after the test meal, plasma monounsaturated GD3 was 75% higher, plasma saturated GD3 was 140% higher and plasma polyunsaturated GM3 30% lower in diabetic subjects compared to control subjects. At 4 h, chylomicron GD1 was 50% lower in T2DM compared to controls. The proportion of d34:1 in GD3 was more abundant and d36:1 in GD1 less abundant in T2DM compared to control subjects at 4 h. The present study indicates that T2DM alters ceramide composition of ganglioside available for uptake by peripheral tissues. |
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| id | doaj.art-699f7c5daebe4549b2cf99d86f40b2e0 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T17:18:13Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-699f7c5daebe4549b2cf99d86f40b2e02023-11-24T13:27:33ZengMDPI AGBiomedicines2227-90592022-12-011012314110.3390/biomedicines10123141Interorgan Metabolism of Ganglioside Is Altered in Type 2 DiabetesIrma Magaly Rivas Serna0Michelle Beveridge1Michaelann Wilke2Edmond A. Ryan3Michael Thomas Clandinin4Vera Christine Mazurak5Division of Human Nutrition, Department of Agricultural, Food and Nutritional Science, Li Ka Shing Centre for Health Research Innovation, University of Alberta, Edmonton, AB T6G 2P5, CanadaDivision of Human Nutrition, Department of Agricultural, Food and Nutritional Science, Li Ka Shing Centre for Health Research Innovation, University of Alberta, Edmonton, AB T6G 2P5, CanadaDivision of Human Nutrition, Department of Agricultural, Food and Nutritional Science, Li Ka Shing Centre for Health Research Innovation, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartment of Medicine, University of Alberta, Edmonton, AB T6G 2R7, CanadaDivision of Human Nutrition, Department of Agricultural, Food and Nutritional Science, Li Ka Shing Centre for Health Research Innovation, University of Alberta, Edmonton, AB T6G 2P5, CanadaDivision of Human Nutrition, Department of Agricultural, Food and Nutritional Science, Li Ka Shing Centre for Health Research Innovation, University of Alberta, Edmonton, AB T6G 2P5, CanadaGM3 is implicated in cell signaling, inflammation and insulin resistance. The intestinal mucosa metabolizes ganglioside and provides gangliosides for uptake by peripheral tissues. Gangliosides downregulate acute and chronic inflammatory signals. It is likely that transport of intestinal derived gangliosides to other tissues impact the same signals characteristic of inflammatory change in other chronic conditions such as Type 2 Diabetes (T2DM). The postprandial ceramide composition of GM3 and other gangliosides in plasma and chylomicrons has not been examined in T2DM. The present study assessed if diet or T2DM alters ganglioside components in plasma and chylomicrons secreted from the intestinal mucosa after a meal. GD1, GD3, and GM3 content of chylomicrons and plasma was determined by LC/triple quad MS in non-diabetic (control) and T2DM individuals in the fasting and postprandial state after 2 days of consuming a low or high fat diet in a randomized blinded crossover design. Diet fat level did not alter baseline plasma or chylomicron ganglioside levels. Four hours after the test meal, plasma monounsaturated GD3 was 75% higher, plasma saturated GD3 was 140% higher and plasma polyunsaturated GM3 30% lower in diabetic subjects compared to control subjects. At 4 h, chylomicron GD1 was 50% lower in T2DM compared to controls. The proportion of d34:1 in GD3 was more abundant and d36:1 in GD1 less abundant in T2DM compared to control subjects at 4 h. The present study indicates that T2DM alters ceramide composition of ganglioside available for uptake by peripheral tissues.https://www.mdpi.com/2227-9059/10/12/3141GD1GM3GD3postprandial perioddiabetesLC/triple quad MS |
| spellingShingle | Irma Magaly Rivas Serna Michelle Beveridge Michaelann Wilke Edmond A. Ryan Michael Thomas Clandinin Vera Christine Mazurak Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes Biomedicines GD1 GM3 GD3 postprandial period diabetes LC/triple quad MS |
| title | Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes |
| title_full | Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes |
| title_fullStr | Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes |
| title_full_unstemmed | Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes |
| title_short | Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes |
| title_sort | interorgan metabolism of ganglioside is altered in type 2 diabetes |
| topic | GD1 GM3 GD3 postprandial period diabetes LC/triple quad MS |
| url | https://www.mdpi.com/2227-9059/10/12/3141 |
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