| Summary: | High-temperature and high-light are the main adversity stresses faced by strawberry seedlings and open-field cultivation in summer. Chitosan (CTS) is widely used in agriculture as a soil amendment, plant growth regulator, and promoter of cold resistance. However, the effects of CTS on strawberry seedlings under the combined stress of high-temperature and high-light are unclear. This study aimed to determine the effect of CTS on strawberry seedlings under the combined stress of high-temperature and high-light. In this study, Xuelixiang strawberry was used as the experimental plant material. The leaves were sprayed with 100 mg/Kg CTS or deionized aqueous solution and placed under high-temperature and high-light (38 °C, 1800 μ mol·m<sup>−2</sup>·s<sup>−1</sup>, respectively) for 8 h. In addition, a suitable temperature and light (23 °C, 400 μ mol·m<sup>−2</sup>·s<sup>−1</sup>, respectively) was sprayed with deionized water as a control. The results showed that compared with non-CTS treatment, the chlorophyll (Chl) content in strawberry plants increased by 16.9% after CTS treatment; the net CO<sub>2</sub> exchange (Pn) increased by 74.9%; and the maximum photochemical efficiency increased by 20.1%. In addition, CTS treatment increased the content of antioxidants; increased osmotic adjustment substances; improved the activity of reduced glutathione with reduced ascorbic acid (AsA-GSH) circulating antioxidant enzymes; removed reactive oxygen species in plants in time; and reduced the damage of reactive oxygen species to photosynthetic organs and cell membranes, thereby reducing high-temperature and high-light injury to strawberry seedlings. This study shows that CTS can improve the negative effects of high-temperature and high-light stress on strawberry seedlings.
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