Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention
Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity of these stem cells depend on the expression of Chemokines and their receptors. Ex vivo expanded MSCs exhibit homing po...
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| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2014-05-01
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| Series: | International Journal of Pediatrics |
| Subjects: | |
| Online Access: | http://ijp.mums.ac.ir/pdf_2668_10d3d7047033ab962e4ec38d2e9ed8cd.html |
| _version_ | 1798040467038797824 |
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| author | Hojjat Naderi-Meshkin Maryam M. Matin Asieh Heirani-Tabasi Maliheh Hasanzadeh Mina Shahryari Naghmeh Ahmadiankia Mahmood Raisolmohaddesin Nasser Sanjar Moussavi Hamid Reza Bidkhori Mahmoud Hosseini Ahmad Reza Bahrami |
| author_facet | Hojjat Naderi-Meshkin Maryam M. Matin Asieh Heirani-Tabasi Maliheh Hasanzadeh Mina Shahryari Naghmeh Ahmadiankia Mahmood Raisolmohaddesin Nasser Sanjar Moussavi Hamid Reza Bidkhori Mahmoud Hosseini Ahmad Reza Bahrami |
| author_sort | Hojjat Naderi-Meshkin |
| collection | DOAJ |
| description | Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity of these stem cells depend on the expression of Chemokines and their receptors. Ex vivo expanded MSCs exhibit homing potential when grafted to injury tissue but their homing efficiency has been observed very poor because of modifications in homing receptor expression and/or functions during culture and/or preparation steps. Hence, this study was designed to investigate the expression of surface CXCR4 by flow cytometric analysis (FACS) and in vitro modified Boyden chamber assay in adipose-derive MSCs (ASCs) stimulated with a hypoxia mimicking agents such as desferrioxamine mesilate (DFX), cobalt chloride (CoCl2), lithium chloride (LiCl), valproic acid (VPA) and hypoxia. Intracellular CXCR4 were also evaluated by conventional and real-time PCR. Then we evaluated the homing ability of DFX-pretreated human DiI-labeled ASCs in vivo, 2 weeks after intravenous (IV), local infusion towards subcutaneously implanted chitosan-glycerophophate-hydroxyethyl cellulose (CH-GP-HEC) injectable hydrogels releasing SDF1 in dorsum of Wistar Rats. Presence of human ASCs in the CH-GP-HEC injectable, spleen, and lung were analyzed histologically by fluorescent microscope, and also quantified by PCR for human specific CXCR4 gene, 2 weeks after transplantation in recipients' Rats. Results showed that short-term (24 hours) pretreatment to ASCs with the hypoxia mimicking agents up-regulate the CXCR4, increase in vitro migration capacity toward 100ng/ml SDF-1 (P |
| first_indexed | 2024-04-11T22:07:59Z |
| format | Article |
| id | doaj.art-69a3170d5f614f68a7b26a9d755aaf76 |
| institution | Directory Open Access Journal |
| issn | 2345-5047 2345-5055 |
| language | English |
| last_indexed | 2024-04-11T22:07:59Z |
| publishDate | 2014-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | International Journal of Pediatrics |
| spelling | doaj.art-69a3170d5f614f68a7b26a9d755aaf762022-12-22T04:00:39ZengMashhad University of Medical SciencesInternational Journal of Pediatrics2345-50472345-50552014-05-0122.319192668Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and RetentionHojjat Naderi-Meshkin0Maryam M. Matin1Asieh Heirani-Tabasi2Maliheh Hasanzadeh3Mina Shahryari4Naghmeh Ahmadiankia5Mahmood Raisolmohaddesin6Nasser Sanjar Moussavi7Hamid Reza Bidkhori8Mahmoud Hosseini9Ahmad Reza Bahrami10Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran.Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.Department of Surgery, Faculty of Medicine, Islamic Azad University-Mashhad Branch, Iran.Stem Cell and Regenerative Medicine Research group, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran.Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity of these stem cells depend on the expression of Chemokines and their receptors. Ex vivo expanded MSCs exhibit homing potential when grafted to injury tissue but their homing efficiency has been observed very poor because of modifications in homing receptor expression and/or functions during culture and/or preparation steps. Hence, this study was designed to investigate the expression of surface CXCR4 by flow cytometric analysis (FACS) and in vitro modified Boyden chamber assay in adipose-derive MSCs (ASCs) stimulated with a hypoxia mimicking agents such as desferrioxamine mesilate (DFX), cobalt chloride (CoCl2), lithium chloride (LiCl), valproic acid (VPA) and hypoxia. Intracellular CXCR4 were also evaluated by conventional and real-time PCR. Then we evaluated the homing ability of DFX-pretreated human DiI-labeled ASCs in vivo, 2 weeks after intravenous (IV), local infusion towards subcutaneously implanted chitosan-glycerophophate-hydroxyethyl cellulose (CH-GP-HEC) injectable hydrogels releasing SDF1 in dorsum of Wistar Rats. Presence of human ASCs in the CH-GP-HEC injectable, spleen, and lung were analyzed histologically by fluorescent microscope, and also quantified by PCR for human specific CXCR4 gene, 2 weeks after transplantation in recipients' Rats. Results showed that short-term (24 hours) pretreatment to ASCs with the hypoxia mimicking agents up-regulate the CXCR4, increase in vitro migration capacity toward 100ng/ml SDF-1 (Phttp://ijp.mums.ac.ir/pdf_2668_10d3d7047033ab962e4ec38d2e9ed8cd.htmlOral Presentation |
| spellingShingle | Hojjat Naderi-Meshkin Maryam M. Matin Asieh Heirani-Tabasi Maliheh Hasanzadeh Mina Shahryari Naghmeh Ahmadiankia Mahmood Raisolmohaddesin Nasser Sanjar Moussavi Hamid Reza Bidkhori Mahmoud Hosseini Ahmad Reza Bahrami Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention International Journal of Pediatrics Oral Presentation |
| title | Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention |
| title_full | Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention |
| title_fullStr | Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention |
| title_full_unstemmed | Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention |
| title_short | Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention |
| title_sort | pretreatment of mesenchymal stem cells and stromal derived factor 1α delivery from chitosan based injectable hydrogels for better cell guidance and retention |
| topic | Oral Presentation |
| url | http://ijp.mums.ac.ir/pdf_2668_10d3d7047033ab962e4ec38d2e9ed8cd.html |
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