Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and Resistin
Objective:Cystic echinococcosis (CE) is one of the most common zoonotic diseases worldwide. Diagnosis of CE is predominantly based on imaging techniques and serological tests are used in cases of non-characteristic imaging findings as diagnostic reference. However, serological test results cannot be...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Yayinevi
2022-09-01
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| Series: | Türkiye Parazitoloji Dergisi |
| Subjects: | |
| Online Access: |
http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/evaluation-of-potential-nflammatory-markers-for-cy/52905
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| _version_ | 1797908748883197952 |
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| author | Serra Örsten İpek Baysal Türkmen Çiftçi Emre Ünal Samiye Yabanoğlu Çiftçi Ahmet Bülent Doğrul Devrim Akıncı Yakut Akyön Okan Akhan |
| author_facet | Serra Örsten İpek Baysal Türkmen Çiftçi Emre Ünal Samiye Yabanoğlu Çiftçi Ahmet Bülent Doğrul Devrim Akıncı Yakut Akyön Okan Akhan |
| author_sort | Serra Örsten |
| collection | DOAJ |
| description | Objective:Cystic echinococcosis (CE) is one of the most common zoonotic diseases worldwide. Diagnosis of CE is predominantly based on imaging techniques and serological tests are used in cases of non-characteristic imaging findings as diagnostic reference. However, serological test results cannot be completely reliable as they are affected by multi-factors. P-selectin and resistin are inflammatory markers that are altered during the acute stages of infection. In this purpose, inflammatory markers as P-selectin and resistin have been investigated for a potential diagnostic reference for CE diagnosis.Methods:A total of 60 patients who were diagnosed with CE and twenty-five healthy individuals were included in this study. Blood samples were obtained from all participants. Obtained sera were evaluated using the P-selectin and resistin ELISA kits for protein levels. Additionally, the relative expression of SELP (P-selectin) and RETN (resistin) genes were determined using the comparative CT (ΔΔCT) method between groups as CE patients with active and inactive cysts, CE patients and healthy controls.Results:SELP (13.9-fold change, p<0.05) and RETN (8.1-fold change, p<0.05) were differentially expressed in CE patients compared in the control group. Whereas resistin protein levels were significantly higher in CE patients than the healthy controls (p<0.001), the difference in P-selectin protein levels was not significant (p>0.05). There was no difference between active and inactive CE patients in terms of P-selectin and resistin in gene and protein levels (p>0.05).Conclusion:Although there was no difference between the active and inactive CE patients, the good differentiation between the healthy controls and the CE patients suggested that resistin is a potential inflammatory diagnostic reference. |
| first_indexed | 2024-04-10T10:57:29Z |
| format | Article |
| id | doaj.art-69a6e9bdd2304a859873d30f20b222ee |
| institution | Directory Open Access Journal |
| issn | 1300-6320 2146-3077 |
| language | English |
| last_indexed | 2024-04-10T10:57:29Z |
| publishDate | 2022-09-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türkiye Parazitoloji Dergisi |
| spelling | doaj.art-69a6e9bdd2304a859873d30f20b222ee2023-02-15T16:19:51ZengGalenos YayineviTürkiye Parazitoloji Dergisi1300-63202146-30772022-09-0146319520010.4274/tpd.galenos.2022.5526513049054Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and ResistinSerra Örsten0İpek Baysal1Türkmen Çiftçi2Emre Ünal3Samiye Yabanoğlu Çiftçi4Ahmet Bülent Doğrul5Devrim Akıncı6Yakut Akyön7Okan Akhan8 Hacettepe University Vocational School of Health Services, Ankara, Turkey Hacettepe University Vocational School of Health Services, Ankara, Turkey Hacettepe University Faculty of Medicine, Department of Radiology, Ankara, Turkey Hacettepe University Faculty of Medicine, Department of Radiology, Ankara, Turkey Hacettepe University Faculty of Pharmacy, Department of Biochemistry, Ankara, Turkey Hacettepe University Faculty of Medicine, Department of General Surgery, Ankara, Turkey Hacettepe University Faculty of Medicine, Department of Radiology, Ankara, Turkey Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey Hacettepe University Faculty of Medicine, Department of Radiology, Ankara, Turkey Objective:Cystic echinococcosis (CE) is one of the most common zoonotic diseases worldwide. Diagnosis of CE is predominantly based on imaging techniques and serological tests are used in cases of non-characteristic imaging findings as diagnostic reference. However, serological test results cannot be completely reliable as they are affected by multi-factors. P-selectin and resistin are inflammatory markers that are altered during the acute stages of infection. In this purpose, inflammatory markers as P-selectin and resistin have been investigated for a potential diagnostic reference for CE diagnosis.Methods:A total of 60 patients who were diagnosed with CE and twenty-five healthy individuals were included in this study. Blood samples were obtained from all participants. Obtained sera were evaluated using the P-selectin and resistin ELISA kits for protein levels. Additionally, the relative expression of SELP (P-selectin) and RETN (resistin) genes were determined using the comparative CT (ΔΔCT) method between groups as CE patients with active and inactive cysts, CE patients and healthy controls.Results:SELP (13.9-fold change, p<0.05) and RETN (8.1-fold change, p<0.05) were differentially expressed in CE patients compared in the control group. Whereas resistin protein levels were significantly higher in CE patients than the healthy controls (p<0.001), the difference in P-selectin protein levels was not significant (p>0.05). There was no difference between active and inactive CE patients in terms of P-selectin and resistin in gene and protein levels (p>0.05).Conclusion:Although there was no difference between the active and inactive CE patients, the good differentiation between the healthy controls and the CE patients suggested that resistin is a potential inflammatory diagnostic reference. http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/evaluation-of-potential-nflammatory-markers-for-cy/52905 cystic echinococcosisresistinp-selectininflammatory markerhydatid cyst |
| spellingShingle | Serra Örsten İpek Baysal Türkmen Çiftçi Emre Ünal Samiye Yabanoğlu Çiftçi Ahmet Bülent Doğrul Devrim Akıncı Yakut Akyön Okan Akhan Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and Resistin Türkiye Parazitoloji Dergisi cystic echinococcosis resistin p-selectin inflammatory marker hydatid cyst |
| title | Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and Resistin |
| title_full | Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and Resistin |
| title_fullStr | Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and Resistin |
| title_full_unstemmed | Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and Resistin |
| title_short | Evaluation of Potential Inflammatory Markers for Cystic Echinococcosis: P-selectin and Resistin |
| title_sort | evaluation of potential inflammatory markers for cystic echinococcosis p selectin and resistin |
| topic | cystic echinococcosis resistin p-selectin inflammatory marker hydatid cyst |
| url |
http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/evaluation-of-potential-nflammatory-markers-for-cy/52905
|
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