Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis
Abstract Background The hepatopancreas of crustaceans serves as a significant organ for both the synthesis and secretion of digestive enzymes, as well as energy storage. In the event of food shortage, the hepatopancreas can provide energy for survival. To investigate the potential regulatory mechani...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-023-09620-x |
| _version_ | 1797453811796672512 |
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| author | Hongli Liu Yang Feng Ma Yang Ya Huang Minghao Li Yi Geng Ping Ouyang Defang Chen Shiyong Yang Lizi Yin Liangyu Li Xiaoli Huang |
| author_facet | Hongli Liu Yang Feng Ma Yang Ya Huang Minghao Li Yi Geng Ping Ouyang Defang Chen Shiyong Yang Lizi Yin Liangyu Li Xiaoli Huang |
| author_sort | Hongli Liu |
| collection | DOAJ |
| description | Abstract Background The hepatopancreas of crustaceans serves as a significant organ for both the synthesis and secretion of digestive enzymes, as well as energy storage. In the event of food shortage, the hepatopancreas can provide energy for survival. To investigate the potential regulatory mechanisms of the hepatopancreas in response to starvation in Eriocheir Sinensis, transcriptome analysis, histological study and qRT-PCR were performed. Results The results showed that starvation caused a decrease in the hepatopancreas index of E. sinensis, which had certain effects on the tissue structure, metabolism and angiogenesis in the hepatopancreas. In addition, WGCNA and linear regression analysis showed that the genes significantly related to the hepatopancreas index were mainly enriched in the angiogenesis pathway, in which AKT signaling played an important role. Starvation may inhibit AKT signaling pathway by reducing the expression of TGFBI, HSP27, HHEX, and EsPVF1, thereby hindering angiogenesis, promoting apoptosis, and leading to hepatopancreas atrophy. Conclusion These results indicate that AKT plays an important role in the angiogenesis pathway and apoptosis of the starvation induced hepatopancreas index reduction, which is beneficial to further understand the effect of starvation stress on hepatopancreas of Chinese mitten crab. |
| first_indexed | 2024-03-09T15:28:16Z |
| format | Article |
| id | doaj.art-69a82289387845b38d3af149c02084e8 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-09T15:28:16Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-69a82289387845b38d3af149c02084e82023-11-26T12:25:27ZengBMCBMC Genomics1471-21642023-10-0124111010.1186/s12864-023-09620-xStarvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesisHongli Liu0Yang Feng1Ma Yang2Ya Huang3Minghao Li4Yi Geng5Ping Ouyang6Defang Chen7Shiyong Yang8Lizi Yin9Liangyu Li10Xiaoli Huang11Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural UniversityDepartment of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural UniversityFisheries Research Institute, Chengdu Academy of Agriculture and Forestry SciencesDepartment of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural UniversityDepartment of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural UniversityDepartment of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural UniversityDepartment of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural UniversityDepartment of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural UniversityFisheries Research Institute, Chengdu Academy of Agriculture and Forestry SciencesDepartment of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural UniversityAbstract Background The hepatopancreas of crustaceans serves as a significant organ for both the synthesis and secretion of digestive enzymes, as well as energy storage. In the event of food shortage, the hepatopancreas can provide energy for survival. To investigate the potential regulatory mechanisms of the hepatopancreas in response to starvation in Eriocheir Sinensis, transcriptome analysis, histological study and qRT-PCR were performed. Results The results showed that starvation caused a decrease in the hepatopancreas index of E. sinensis, which had certain effects on the tissue structure, metabolism and angiogenesis in the hepatopancreas. In addition, WGCNA and linear regression analysis showed that the genes significantly related to the hepatopancreas index were mainly enriched in the angiogenesis pathway, in which AKT signaling played an important role. Starvation may inhibit AKT signaling pathway by reducing the expression of TGFBI, HSP27, HHEX, and EsPVF1, thereby hindering angiogenesis, promoting apoptosis, and leading to hepatopancreas atrophy. Conclusion These results indicate that AKT plays an important role in the angiogenesis pathway and apoptosis of the starvation induced hepatopancreas index reduction, which is beneficial to further understand the effect of starvation stress on hepatopancreas of Chinese mitten crab.https://doi.org/10.1186/s12864-023-09620-xHepatopancreasStarvationEriocheir sinensisAngiogenesis |
| spellingShingle | Hongli Liu Yang Feng Ma Yang Ya Huang Minghao Li Yi Geng Ping Ouyang Defang Chen Shiyong Yang Lizi Yin Liangyu Li Xiaoli Huang Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis BMC Genomics Hepatopancreas Starvation Eriocheir sinensis Angiogenesis |
| title | Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis |
| title_full | Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis |
| title_fullStr | Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis |
| title_full_unstemmed | Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis |
| title_short | Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis |
| title_sort | starvation induces hepatopancreas atrophy in chinese mitten crab eriocheir sinensis by inhibiting angiogenesis |
| topic | Hepatopancreas Starvation Eriocheir sinensis Angiogenesis |
| url | https://doi.org/10.1186/s12864-023-09620-x |
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