Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes
Overload of hepatic palmitate, like palmitic acid (PA), is known as the key trigger for non-alcoholic fatty liver disease. Here, we investigated whether Chinese propolis (CP) would mitigate PA-induced lipotoxicity in hepatocytes. Total of 20 phenolic compounds were analysed in CP using HPLC-DAD/Q-TO...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617302293 |
| _version_ | 1818953691716124672 |
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| author | Xiao-Lu Jin Kai Wang Qiang-Qiang Li Wen-Li Tian Xiao-Feng Xue Li-Ming Wu Fu-Liang Hu |
| author_facet | Xiao-Lu Jin Kai Wang Qiang-Qiang Li Wen-Li Tian Xiao-Feng Xue Li-Ming Wu Fu-Liang Hu |
| author_sort | Xiao-Lu Jin |
| collection | DOAJ |
| description | Overload of hepatic palmitate, like palmitic acid (PA), is known as the key trigger for non-alcoholic fatty liver disease. Here, we investigated whether Chinese propolis (CP) would mitigate PA-induced lipotoxicity in hepatocytes. Total of 20 phenolic compounds were analysed in CP using HPLC-DAD/Q-TOF-MS. PA has significant cytotoxic effects on HepG2 and L02 hepatocytes, by decreasing cell viability and inducing LDH releases, which was rescued by CP pre-treatment. Lipoapoptosis was also found in HepG2 hepatocytes treated with PA and CP helped restore the energy provision and prevented cell apoptosis. After PA challenges, antioxidant effects of CP were also observed by boosting the HepG2 cellular total-antioxidant potentials, increasing superoxide dismutase level and up-regulating antioxidant/detoxicant gene expressions (GSTA1, TXNRD1, NQO-1, HO-1 and Nrf2). Notably, gene expressions of inflammatory cytokine, TNF-α and IL-8, were decreased by CP. Therefore, CP protects hepatocytes against PA-induced lipotoxicity by lowering biomarkers of apoptosis, oxidative stress and inflammation. |
| first_indexed | 2024-12-20T10:10:18Z |
| format | Article |
| id | doaj.art-69aa7c9135c0419da7fa13dfd512ae55 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T10:10:18Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-69aa7c9135c0419da7fa13dfd512ae552022-12-21T19:44:11ZengElsevierJournal of Functional Foods1756-46462017-07-0134216223Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytesXiao-Lu Jin0Kai Wang1Qiang-Qiang Li2Wen-Li Tian3Xiao-Feng Xue4Li-Ming Wu5Fu-Liang Hu6Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Corresponding authors.Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Corresponding authors.College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaOverload of hepatic palmitate, like palmitic acid (PA), is known as the key trigger for non-alcoholic fatty liver disease. Here, we investigated whether Chinese propolis (CP) would mitigate PA-induced lipotoxicity in hepatocytes. Total of 20 phenolic compounds were analysed in CP using HPLC-DAD/Q-TOF-MS. PA has significant cytotoxic effects on HepG2 and L02 hepatocytes, by decreasing cell viability and inducing LDH releases, which was rescued by CP pre-treatment. Lipoapoptosis was also found in HepG2 hepatocytes treated with PA and CP helped restore the energy provision and prevented cell apoptosis. After PA challenges, antioxidant effects of CP were also observed by boosting the HepG2 cellular total-antioxidant potentials, increasing superoxide dismutase level and up-regulating antioxidant/detoxicant gene expressions (GSTA1, TXNRD1, NQO-1, HO-1 and Nrf2). Notably, gene expressions of inflammatory cytokine, TNF-α and IL-8, were decreased by CP. Therefore, CP protects hepatocytes against PA-induced lipotoxicity by lowering biomarkers of apoptosis, oxidative stress and inflammation.http://www.sciencedirect.com/science/article/pii/S1756464617302293PropolisPolyphenolsLipoapoptosisHepatocytesNAFLD |
| spellingShingle | Xiao-Lu Jin Kai Wang Qiang-Qiang Li Wen-Li Tian Xiao-Feng Xue Li-Ming Wu Fu-Liang Hu Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes Journal of Functional Foods Propolis Polyphenols Lipoapoptosis Hepatocytes NAFLD |
| title | Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes |
| title_full | Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes |
| title_fullStr | Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes |
| title_full_unstemmed | Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes |
| title_short | Antioxidant and anti-inflammatory effects of Chinese propolis during palmitic acid-induced lipotoxicity in cultured hepatocytes |
| title_sort | antioxidant and anti inflammatory effects of chinese propolis during palmitic acid induced lipotoxicity in cultured hepatocytes |
| topic | Propolis Polyphenols Lipoapoptosis Hepatocytes NAFLD |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617302293 |
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