Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice
Lesions to the CNS/PNS interface are especially severe, leading to elevated neuronal degeneration. In the present work, we establish the ventral root crush model for mice, and demonstrate the potential of such an approach, by analyzing injury evoked motoneuron loss, changes of synaptic coverage and...
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| Format: | Article |
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MDPI AG
2019-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/5/483 |
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| author | Luciana Politti Cartarozzi Matheus Perez Frank Kirchhoff Alexandre Leite Rodrigues de Oliveira |
| author_facet | Luciana Politti Cartarozzi Matheus Perez Frank Kirchhoff Alexandre Leite Rodrigues de Oliveira |
| author_sort | Luciana Politti Cartarozzi |
| collection | DOAJ |
| description | Lesions to the CNS/PNS interface are especially severe, leading to elevated neuronal degeneration. In the present work, we establish the ventral root crush model for mice, and demonstrate the potential of such an approach, by analyzing injury evoked motoneuron loss, changes of synaptic coverage and concomitant glial responses in β2-microglobulin knockout mice (β2m KO). Young adult (8−12 weeks old) C57BL/6J (WT) and β2m KO mice were submitted to a L4−L6 ventral roots crush. Neuronal survival revealed a time-dependent motoneuron-like cell loss, both in WT and β2m KO mice. Along with neuronal loss, astrogliosis increased in WT mice, which was not observed in β2m KO mice. Microglial responses were more pronounced during the acute phase after lesion and decreased over time, in WT and KO mice. At 7 days after lesion β2m KO mice showed stronger Iba-1<sup>+</sup> cell reaction. The synaptic inputs were reduced over time, but in β2m KO, the synaptic loss was more prominent between 7 and 28 days after lesion. Taken together, the results herein demonstrate that ventral root crushing in mice provides robust data regarding neuronal loss and glial reaction. The retrograde reactions after injury were altered in the absence of functional MHC-I surface expression. |
| first_indexed | 2024-03-12T06:21:46Z |
| format | Article |
| id | doaj.art-69ac21ba1c684802a6ae45026f85e8b6 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:21:46Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-69ac21ba1c684802a6ae45026f85e8b62023-09-03T02:11:06ZengMDPI AGCells2073-44092019-05-018548310.3390/cells8050483cells8050483Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in MiceLuciana Politti Cartarozzi0Matheus Perez1Frank Kirchhoff2Alexandre Leite Rodrigues de Oliveira3Laboratory of Nerve Regeneration, University of Campinas–UNICAMP, Cidade Universitaria “Zeferino Vaz, Rua Monteiro Lobato, 255, 13083-970 Campinas, SP, BrazilSchool of Physical Education and Sport of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900, 14040-907 Ribeirão Preto, SP, BrazilMolecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, Building 48, 66421 Homburg, GermanyLaboratory of Nerve Regeneration, University of Campinas–UNICAMP, Cidade Universitaria “Zeferino Vaz, Rua Monteiro Lobato, 255, 13083-970 Campinas, SP, BrazilLesions to the CNS/PNS interface are especially severe, leading to elevated neuronal degeneration. In the present work, we establish the ventral root crush model for mice, and demonstrate the potential of such an approach, by analyzing injury evoked motoneuron loss, changes of synaptic coverage and concomitant glial responses in β2-microglobulin knockout mice (β2m KO). Young adult (8−12 weeks old) C57BL/6J (WT) and β2m KO mice were submitted to a L4−L6 ventral roots crush. Neuronal survival revealed a time-dependent motoneuron-like cell loss, both in WT and β2m KO mice. Along with neuronal loss, astrogliosis increased in WT mice, which was not observed in β2m KO mice. Microglial responses were more pronounced during the acute phase after lesion and decreased over time, in WT and KO mice. At 7 days after lesion β2m KO mice showed stronger Iba-1<sup>+</sup> cell reaction. The synaptic inputs were reduced over time, but in β2m KO, the synaptic loss was more prominent between 7 and 28 days after lesion. Taken together, the results herein demonstrate that ventral root crushing in mice provides robust data regarding neuronal loss and glial reaction. The retrograde reactions after injury were altered in the absence of functional MHC-I surface expression.https://www.mdpi.com/2073-4409/8/5/483astrogliosisPNS/CNS interfacemicroglial reactionsynaptic coveringβ2m knockout mice |
| spellingShingle | Luciana Politti Cartarozzi Matheus Perez Frank Kirchhoff Alexandre Leite Rodrigues de Oliveira Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice Cells astrogliosis PNS/CNS interface microglial reaction synaptic covering β2m knockout mice |
| title | Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice |
| title_full | Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice |
| title_fullStr | Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice |
| title_full_unstemmed | Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice |
| title_short | Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice |
| title_sort | role of mhc i expression on spinal motoneuron survival and glial reactions following ventral root crush in mice |
| topic | astrogliosis PNS/CNS interface microglial reaction synaptic covering β2m knockout mice |
| url | https://www.mdpi.com/2073-4409/8/5/483 |
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