The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss
Recurrent pregnancy loss (RPL) is a common fertility problem that affects 1%-2% of couples all over the world. Despite exciting discoveries regarding the important roles of the decidual natural killer cell (dNK) and regulatory T cell in pregnancy, the immune heterogeneity in patients with unexplaine...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.689019/full |
| _version_ | 1818643682422685696 |
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| author | Pengfei Chen Pengfei Chen Liying Zhou Jiying Chen Ying Lu Chaoxia Cao Shuangli Lv Zhihong Wei Zhihong Wei Liping Wang Jiao Chen Xinglin Hu Zijing Wu Xiaohua Zhou Danna Su Xuefeng Deng Changchun Zeng Huiyun Wang Zuhui Pu Ruiying Diao Lisha Mou |
| author_facet | Pengfei Chen Pengfei Chen Liying Zhou Jiying Chen Ying Lu Chaoxia Cao Shuangli Lv Zhihong Wei Zhihong Wei Liping Wang Jiao Chen Xinglin Hu Zijing Wu Xiaohua Zhou Danna Su Xuefeng Deng Changchun Zeng Huiyun Wang Zuhui Pu Ruiying Diao Lisha Mou |
| author_sort | Pengfei Chen |
| collection | DOAJ |
| description | Recurrent pregnancy loss (RPL) is a common fertility problem that affects 1%-2% of couples all over the world. Despite exciting discoveries regarding the important roles of the decidual natural killer cell (dNK) and regulatory T cell in pregnancy, the immune heterogeneity in patients with unexplained recurrent pregnancy loss (URPL) remains elusive. Here, we profiled the transcriptomes of 13,953 CD45+ cells from three normal and three URPL deciduas. Based on our data, the cellular composition revealed three major populations of immune cells including dNK cell, T cell, and macrophage, and four minor populations including monocytes, dendritic cell (DC), mast cell, and B cell. Especially, we identified a subpopulation of CSF1+ CD59+ KIRs-expressing dNK cells in normal deciduas, while the proportion of this subpopulation was decreased in URPL deciduas. We also identified a small subpopulation of activated dDCs that were accumulated mainly in URPL deciduas. Furthermore, our data revealed that in decidua at early pregnancy, CD8+ T cells exhibited cytotoxic properties. The decidual macrophages expressed high levels of both M1 and M2 feature genes, which made them unique to the conventional M1/M2 classification. Our single-cell data revealed the immune heterogeneity in decidua and the potentially pathogenic immune variations in URPL. |
| first_indexed | 2024-12-17T00:02:50Z |
| format | Article |
| id | doaj.art-69ae4d7fa56b46289c52714fc1ed1b42 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-17T00:02:50Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-69ae4d7fa56b46289c52714fc1ed1b422022-12-21T22:11:01ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-06-011210.3389/fimmu.2021.689019689019The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy LossPengfei Chen0Pengfei Chen1Liying Zhou2Jiying Chen3Ying Lu4Chaoxia Cao5Shuangli Lv6Zhihong Wei7Zhihong Wei8Liping Wang9Jiao Chen10Xinglin Hu11Zijing Wu12Xiaohua Zhou13Danna Su14Xuefeng Deng15Changchun Zeng16Huiyun Wang17Zuhui Pu18Ruiying Diao19Lisha Mou20Department of Traumatic Orthopedics, Shenzhen Longhua District Central Hospital, Shenzhen, ChinaShenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Gynaecology, Shenzhen Longhua District Central Hospital, Shenzhen, ChinaDepartment of Gynaecology, Shenzhen Longhua District Central Hospital, Shenzhen, ChinaShenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Gynaecology, Shenzhen Longhua District Central Hospital, Shenzhen, ChinaDepartment of Gynaecology, Shenzhen Longhua District Central Hospital, Shenzhen, ChinaDepartment of Gynaecology, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Gynaecology, Shenzhen Baoan People’s Hospital (Group), Shenzhen, ChinaCentre of Reproductive Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaShenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaShenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaShenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Gynaecology, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaCentre of Reproductive Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Traumatic Orthopedics, Shenzhen Longhua District Central Hospital, Shenzhen, ChinaDepartment of Traumatic Orthopedics, Shenzhen Longhua District Central Hospital, Shenzhen, ChinaState Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, ChinaDepartment of Radiology, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaCentre of Reproductive Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaShenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaRecurrent pregnancy loss (RPL) is a common fertility problem that affects 1%-2% of couples all over the world. Despite exciting discoveries regarding the important roles of the decidual natural killer cell (dNK) and regulatory T cell in pregnancy, the immune heterogeneity in patients with unexplained recurrent pregnancy loss (URPL) remains elusive. Here, we profiled the transcriptomes of 13,953 CD45+ cells from three normal and three URPL deciduas. Based on our data, the cellular composition revealed three major populations of immune cells including dNK cell, T cell, and macrophage, and four minor populations including monocytes, dendritic cell (DC), mast cell, and B cell. Especially, we identified a subpopulation of CSF1+ CD59+ KIRs-expressing dNK cells in normal deciduas, while the proportion of this subpopulation was decreased in URPL deciduas. We also identified a small subpopulation of activated dDCs that were accumulated mainly in URPL deciduas. Furthermore, our data revealed that in decidua at early pregnancy, CD8+ T cells exhibited cytotoxic properties. The decidual macrophages expressed high levels of both M1 and M2 feature genes, which made them unique to the conventional M1/M2 classification. Our single-cell data revealed the immune heterogeneity in decidua and the potentially pathogenic immune variations in URPL.https://www.frontiersin.org/articles/10.3389/fimmu.2021.689019/fullunexplained recurrent pregnancy losshuman deciduaimmune heterogeneitysingle-cell RNA sequencingscRNA decidual nature killer cellthe immune atlas |
| spellingShingle | Pengfei Chen Pengfei Chen Liying Zhou Jiying Chen Ying Lu Chaoxia Cao Shuangli Lv Zhihong Wei Zhihong Wei Liping Wang Jiao Chen Xinglin Hu Zijing Wu Xiaohua Zhou Danna Su Xuefeng Deng Changchun Zeng Huiyun Wang Zuhui Pu Ruiying Diao Lisha Mou The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss Frontiers in Immunology unexplained recurrent pregnancy loss human decidua immune heterogeneity single-cell RNA sequencing scRNA decidual nature killer cell the immune atlas |
| title | The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss |
| title_full | The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss |
| title_fullStr | The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss |
| title_full_unstemmed | The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss |
| title_short | The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss |
| title_sort | immune atlas of human deciduas with unexplained recurrent pregnancy loss |
| topic | unexplained recurrent pregnancy loss human decidua immune heterogeneity single-cell RNA sequencing scRNA decidual nature killer cell the immune atlas |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.689019/full |
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