Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China
<i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Derby (<i>S.</i> Derby) is one of the numerous non-typhoidal <i>Salmonella</i> serovars and has been recognized as a food-borne pathogen. In 2019, outbreaks of salmonellosis were reported in 13...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/8/2397 |
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| author | Xue Fu Lan Feng Linghan Kong Chun Li Xiaodong Zhao Huade Li Pengfei Cui Wenjun Yan Yaru Zhai Lan Zhang Hao Li Hongning Wang Xin Yang |
| author_facet | Xue Fu Lan Feng Linghan Kong Chun Li Xiaodong Zhao Huade Li Pengfei Cui Wenjun Yan Yaru Zhai Lan Zhang Hao Li Hongning Wang Xin Yang |
| author_sort | Xue Fu |
| collection | DOAJ |
| description | <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Derby (<i>S.</i> Derby) is one of the numerous non-typhoidal <i>Salmonella</i> serovars and has been recognized as a food-borne pathogen. In 2019, outbreaks of salmonellosis were reported in 13 yak farms in the Aba Tibetan Autonomous Prefecture, China. A total of 32 salmonella strains were isolated from 162 fecal samples of yaks with diarrhea as well as from drinking water samples. The isolates were subjected to serovar identification, animal experiments, and whole-genome sequencing (WGS) analyses. The serovar of all the isolates was <i>S.</i> Derby, and the sequence types (STs) were ST40. The analysis of the differences of single-nucleotide polymorphisms (SNPs) showed that the salmonella strains isolated from 13 farms were clonally related. Animal experiments showed that the lethal dose (LD<sub>50</sub>) was 4.57 × 10<sup>7</sup> CFU (colony-forming units); the shedding time of <i>S.</i> Derby in mice was 24 days; the bacterial loads in spleen were higher than those in other organs (ileum, liver, and cecum). Pathological analyses by hematoxylin and eosin (H&E) staining revealed obvious damage in the spleen, liver, and intestine. These results indicate that the <i>S.</i> Derby from yaks can cause infection in mice. |
| first_indexed | 2024-03-10T09:04:20Z |
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| id | doaj.art-69b64025f06a445bbcc2106495bafaf4 |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:04:20Z |
| publishDate | 2021-08-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-69b64025f06a445bbcc2106495bafaf42023-11-22T06:31:32ZengMDPI AGAnimals2076-26152021-08-01118239710.3390/ani11082397Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, ChinaXue Fu0Lan Feng1Linghan Kong2Chun Li3Xiaodong Zhao4Huade Li5Pengfei Cui6Wenjun Yan7Yaru Zhai8Lan Zhang9Hao Li10Hongning Wang11Xin Yang12Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaChengdu Centre for Disease Prevention and Control, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaSichuan Animal Disease Control Center, Chengdu 610064, ChinaSichuan Longri Breeding Stock Farm, Hongyuan 624400, ChinaSichuan Academy of Grassland Sciences, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China<i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Derby (<i>S.</i> Derby) is one of the numerous non-typhoidal <i>Salmonella</i> serovars and has been recognized as a food-borne pathogen. In 2019, outbreaks of salmonellosis were reported in 13 yak farms in the Aba Tibetan Autonomous Prefecture, China. A total of 32 salmonella strains were isolated from 162 fecal samples of yaks with diarrhea as well as from drinking water samples. The isolates were subjected to serovar identification, animal experiments, and whole-genome sequencing (WGS) analyses. The serovar of all the isolates was <i>S.</i> Derby, and the sequence types (STs) were ST40. The analysis of the differences of single-nucleotide polymorphisms (SNPs) showed that the salmonella strains isolated from 13 farms were clonally related. Animal experiments showed that the lethal dose (LD<sub>50</sub>) was 4.57 × 10<sup>7</sup> CFU (colony-forming units); the shedding time of <i>S.</i> Derby in mice was 24 days; the bacterial loads in spleen were higher than those in other organs (ileum, liver, and cecum). Pathological analyses by hematoxylin and eosin (H&E) staining revealed obvious damage in the spleen, liver, and intestine. These results indicate that the <i>S.</i> Derby from yaks can cause infection in mice.https://www.mdpi.com/2076-2615/11/8/2397<i>Salmonella</i> DerbyyakdiarrheaSNP analysisvirulence |
| spellingShingle | Xue Fu Lan Feng Linghan Kong Chun Li Xiaodong Zhao Huade Li Pengfei Cui Wenjun Yan Yaru Zhai Lan Zhang Hao Li Hongning Wang Xin Yang Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China Animals <i>Salmonella</i> Derby yak diarrhea SNP analysis virulence |
| title | Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China |
| title_full | Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China |
| title_fullStr | Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China |
| title_full_unstemmed | Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China |
| title_short | Prevalence, Characterization, and Pathogenicity of <i>Salmonella enterica</i> Subspecies <i>enterica</i> Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China |
| title_sort | prevalence characterization and pathogenicity of i salmonella enterica i subspecies i enterica i serovar derby from yaks in the aba tibetan autonomous prefecture china |
| topic | <i>Salmonella</i> Derby yak diarrhea SNP analysis virulence |
| url | https://www.mdpi.com/2076-2615/11/8/2397 |
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