Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii Isolates
Background & Objectives : Acinetobacter baumannii is an opportunistic Gram-negative pathogen with increasing relevance in a variety of hospital-acquired infections especially among intensive care unit patients. A. baumannii is mostly a cause of septicemia, pneumonia and urinary tract infection f...
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Ardabil University of Medical Sciences
2014-04-01
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| Series: | Journal of Ardabil University of Medical Sciences |
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| Online Access: | http://jarums.arums.ac.ir/browse.php?a_code=A-10-27-223&slc_lang=en&sid=1 |
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| author | Delsuz Rezaee Gholamreza Zarrini Mohammad Ahangarzadeh Rezaee |
| author_facet | Delsuz Rezaee Gholamreza Zarrini Mohammad Ahangarzadeh Rezaee |
| author_sort | Delsuz Rezaee |
| collection | DOAJ |
| description | Background & Objectives : Acinetobacter baumannii is an opportunistic Gram-negative pathogen with increasing relevance in a variety of hospital-acquired infections especially among intensive care unit patients. A. baumannii is mostly a cause of septicemia, pneumonia and urinary tract infection following hospitalization of patients. In this study antibiotic susceptibility pattern of A.baumannii isolates and molecular typing among isolates resistant by REP-PCR were determined. Methods : During study, the A. baumannii, were isolated from hospitals in Tehran. The isolates were identified using standard biochemical tests and antibiotic susceptibility was determined by the disk diffusion method. Extraction of DNA and molecular typing of isolates performed using CTAB method and REP-PCR, respectively. Results : In this study 75 A. baumannii isolates separated from patients with an average age of 51 ± 18.45 years . The highest resistance rate was against azteronam (97%), ceftazidim (93%), cefepime (93%), piperacillin-tazobactam (93%), ciprofloxacin (93%) and ticarcillin (93%) while the lowest resistance rate was against tigecycline (n= 51, 68%), followed by tobramycin (n=24, 32%), ampicillin-sulbactam (n=21, 28%), amikacin (n=16, 21%), and carbapenems (n=11, 15%). The REP-PCR in resistant of A. baumannii isolates showed that the genotypes of A, B and C are the predominant genotypes in the resistant antibiotics. Conclusion: This study showed a high percentage of resistance to antimicrobial agents among genotypes A, B, and C of the A. baumannii isolates therefore strategies to control the spread of A. baumannii isolates must be designed and evaluated. |
| first_indexed | 2024-12-10T21:26:33Z |
| format | Article |
| id | doaj.art-69b701e48bb944679dc52f7f548cd0e8 |
| institution | Directory Open Access Journal |
| issn | 2228-7280 2228-7299 |
| language | fas |
| last_indexed | 2024-12-10T21:26:33Z |
| publishDate | 2014-04-01 |
| publisher | Ardabil University of Medical Sciences |
| record_format | Article |
| series | Journal of Ardabil University of Medical Sciences |
| spelling | doaj.art-69b701e48bb944679dc52f7f548cd0e82022-12-22T01:32:58ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992014-04-011412836Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii IsolatesDelsuz Rezaee0Gholamreza Zarrini1Mohammad Ahangarzadeh Rezaee2 Background & Objectives : Acinetobacter baumannii is an opportunistic Gram-negative pathogen with increasing relevance in a variety of hospital-acquired infections especially among intensive care unit patients. A. baumannii is mostly a cause of septicemia, pneumonia and urinary tract infection following hospitalization of patients. In this study antibiotic susceptibility pattern of A.baumannii isolates and molecular typing among isolates resistant by REP-PCR were determined. Methods : During study, the A. baumannii, were isolated from hospitals in Tehran. The isolates were identified using standard biochemical tests and antibiotic susceptibility was determined by the disk diffusion method. Extraction of DNA and molecular typing of isolates performed using CTAB method and REP-PCR, respectively. Results : In this study 75 A. baumannii isolates separated from patients with an average age of 51 ± 18.45 years . The highest resistance rate was against azteronam (97%), ceftazidim (93%), cefepime (93%), piperacillin-tazobactam (93%), ciprofloxacin (93%) and ticarcillin (93%) while the lowest resistance rate was against tigecycline (n= 51, 68%), followed by tobramycin (n=24, 32%), ampicillin-sulbactam (n=21, 28%), amikacin (n=16, 21%), and carbapenems (n=11, 15%). The REP-PCR in resistant of A. baumannii isolates showed that the genotypes of A, B and C are the predominant genotypes in the resistant antibiotics. Conclusion: This study showed a high percentage of resistance to antimicrobial agents among genotypes A, B, and C of the A. baumannii isolates therefore strategies to control the spread of A. baumannii isolates must be designed and evaluated.http://jarums.arums.ac.ir/browse.php?a_code=A-10-27-223&slc_lang=en&sid=1Acinetobacter baumannii; Antibiotic Resistance; molecular Typing; REP-PCR |
| spellingShingle | Delsuz Rezaee Gholamreza Zarrini Mohammad Ahangarzadeh Rezaee Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii Isolates Journal of Ardabil University of Medical Sciences Acinetobacter baumannii; Antibiotic Resistance; molecular Typing; REP-PCR |
| title | Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii Isolates |
| title_full | Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii Isolates |
| title_fullStr | Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii Isolates |
| title_full_unstemmed | Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii Isolates |
| title_short | Antibiotic Susceptibility and Molecular Typing by REP-PCR among Acinetobacter baumannii Isolates |
| title_sort | antibiotic susceptibility and molecular typing by rep pcr among acinetobacter baumannii isolates |
| topic | Acinetobacter baumannii; Antibiotic Resistance; molecular Typing; REP-PCR |
| url | http://jarums.arums.ac.ir/browse.php?a_code=A-10-27-223&slc_lang=en&sid=1 |
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