Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and Characterisation
Thirty isolated fungal strains were screened for lipase production using Phenol Red plates, containing tributyrin as lipidic substrate, and a novel fungus identified genetically as Curvularia sp. DHE 5 was found as the most prominent strain. Various agro-industrial substrates were evaluated as inert...
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University of Zagreb Faculty of Food Technology and Biotechnology
2017-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/269928 |
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| author | Dina Helmy El-Ghonemy Mamdouh S. El-Gamal Amir Elsayed Tantawy Thanaa Hamed Ali |
| author_facet | Dina Helmy El-Ghonemy Mamdouh S. El-Gamal Amir Elsayed Tantawy Thanaa Hamed Ali |
| author_sort | Dina Helmy El-Ghonemy |
| collection | DOAJ |
| description | Thirty isolated fungal strains were screened for lipase production using Phenol Red plates, containing tributyrin as lipidic substrate, and a novel fungus identified genetically as Curvularia sp. DHE 5 was found as the most prominent strain. Various agro-industrial substrates were evaluated as inert supports for lipase production in solid-state fermentation. The highest yield of lipase ((83.4±2.2) U/g on dry mass basis) was reported with wheat bran medium after seven days of fermentation at pH=7.0, temperature of 30 °C, 70 % moisture content, inoculum size of 1.27·107 spore/mL and 2 % olive oil as an inducer. Supplementation of the medium with 0.05 % KCl as an ion source further increased lipase production to (88.9±1.2) U/g on dry mass basis. The enzyme was partially purified through ammonium sulphate fractionation (40 %) followed by dialysis, and its optimum pH and temperature were reported at 8.0 and 50 °C, respectively, with remarkable pH and thermal stability. |
| first_indexed | 2024-04-24T09:29:53Z |
| format | Article |
| id | doaj.art-69b8e85729db4d9f8ae57a45f27b7b1d |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:29:53Z |
| publishDate | 2017-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-69b8e85729db4d9f8ae57a45f27b7b1d2024-04-15T14:12:55ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062017-01-0155220621710.17113/ftb.55.02.17.4958Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and CharacterisationDina Helmy El-Ghonemy0Mamdouh S. El-Gamal1Amir Elsayed Tantawy2Thanaa Hamed Ali3Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Buhouth St., EG-12 622 Giza, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, EG-11 884 Nasr City, EgyptDepartment of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Buhouth St., EG-12 622 Giza, EgyptDepartment of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Buhouth St., EG-12 622 Giza, EgyptThirty isolated fungal strains were screened for lipase production using Phenol Red plates, containing tributyrin as lipidic substrate, and a novel fungus identified genetically as Curvularia sp. DHE 5 was found as the most prominent strain. Various agro-industrial substrates were evaluated as inert supports for lipase production in solid-state fermentation. The highest yield of lipase ((83.4±2.2) U/g on dry mass basis) was reported with wheat bran medium after seven days of fermentation at pH=7.0, temperature of 30 °C, 70 % moisture content, inoculum size of 1.27·107 spore/mL and 2 % olive oil as an inducer. Supplementation of the medium with 0.05 % KCl as an ion source further increased lipase production to (88.9±1.2) U/g on dry mass basis. The enzyme was partially purified through ammonium sulphate fractionation (40 %) followed by dialysis, and its optimum pH and temperature were reported at 8.0 and 50 °C, respectively, with remarkable pH and thermal stability.https://hrcak.srce.hr/file/269928lipaseCurvularia sp. DHE 5optimisation of culture conditionsagro-industrial residueslipase characterisation |
| spellingShingle | Dina Helmy El-Ghonemy Mamdouh S. El-Gamal Amir Elsayed Tantawy Thanaa Hamed Ali Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and Characterisation Food Technology and Biotechnology lipase Curvularia sp. DHE 5 optimisation of culture conditions agro-industrial residues lipase characterisation |
| title | Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and Characterisation |
| title_full | Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and Characterisation |
| title_fullStr | Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and Characterisation |
| title_full_unstemmed | Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and Characterisation |
| title_short | Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimisation of Physicochemical Parameters, Partial Purification and Characterisation |
| title_sort | extracellular alkaline lipase from a novel fungus curvularia sp dhe 5 optimisation of physicochemical parameters partial purification and characterisation |
| topic | lipase Curvularia sp. DHE 5 optimisation of culture conditions agro-industrial residues lipase characterisation |
| url | https://hrcak.srce.hr/file/269928 |
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