Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i>
α-glucosidase is responsible for the hydrolysis of complex carbohydrates into simple absorbable glucose and causes postprandial hyperglycemia. α-glucosidase inhibition is thus the ideal target to prevent postprandial hyperglycemia. The present study was therefore designed to analyze the effects of v...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/3/427 |
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| author | Surriya Amin Barkat Ullah Mumtaz Ali Abdur Rauf Haroon Khan Eugenio Uriarte Eduardo Sobarzo-Sánchez |
| author_facet | Surriya Amin Barkat Ullah Mumtaz Ali Abdur Rauf Haroon Khan Eugenio Uriarte Eduardo Sobarzo-Sánchez |
| author_sort | Surriya Amin |
| collection | DOAJ |
| description | α-glucosidase is responsible for the hydrolysis of complex carbohydrates into simple absorbable glucose and causes postprandial hyperglycemia. α-glucosidase inhibition is thus the ideal target to prevent postprandial hyperglycemia. The present study was therefore designed to analyze the effects of various compounds isolated from <i>Dryopteris cycadina</i> against α-glucosidase including β-Sitosterol <b>1</b>, β-Sitosterol3-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucopyranoside <b>2</b>, 3, 5, 7-trihydroxy-2-(<i>p</i>-tolyl) chorman-4-one <b>3</b>, Quercetin-3-0-β-<span style="font-variant: small-caps">d</span>-glucopyranoside (3<sup>/</sup>→0-3<sup>///</sup>)- β-<span style="font-variant: small-caps">d</span>- Quercetin -3-0- β –<span style="font-variant: small-caps">d</span>-galactopyranoside <b>4</b> and 5, 7, 4<sup>/</sup>-Trihydroxyflavon-3-glucopyranoid 5. The in vitro spectrophotometric method was used for the analysis of test compounds against possible inhibition. Similarly, molecular docking studies were performed using the MOE software. These compounds showed concentration-dependent inhibition on α-glucosidase, and compounds <b>1</b> (IC<sub>50</sub>: 143 ± 0.47 µM), <b>3</b> (IC<sub>50</sub>:133 ± 6.90 µM) and <b>5</b> (IC<sub>50</sub>: 146 ± 1.93 µM) were more potent than the standard drug, acarbose (IC<sub>50</sub>: 290 ± 0.54 µM). Computational studies of these compounds strongly supported the in vitro studies and showed strong binding receptor sensitivity. In short, the secondary metabolites isolated from <i>D. cycadina</i> demonstrated potent α-glucosidase inhibition that were supported by molecular docking with a high docking score. |
| first_indexed | 2024-12-18T19:15:46Z |
| format | Article |
| id | doaj.art-69b8f1248c094e019fb421921a691a90 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-18T19:15:46Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-69b8f1248c094e019fb421921a691a902022-12-21T20:56:08ZengMDPI AGMolecules1420-30492019-01-0124342710.3390/molecules24030427molecules24030427Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i>Surriya Amin0Barkat Ullah1Mumtaz Ali2Abdur Rauf3Haroon Khan4Eugenio Uriarte5Eduardo Sobarzo-Sánchez6Department of Botany, Islamia College University, Peshawar 25000, PakistanDepartment of Botany, Islamia College University, Peshawar 25000, PakistanDepartment of Chemistry, University of Malakand, Upper Dir 23050, PakistanDepartment of Chemistry, University of Swabi, Khyber Pakhtunkhwa, Anbar 23430, PakistanDepartment of Pharmacy, Abdul Wali Khan University, Mardan 23200, PakistanInstituto de Ciencias Químicas Aplicadas, Universidad Autónoma de Chile, Santiago 7500912, ChileInstituto de Investigación e Innovación en Salud, Facultad de Ciencias de la Salud, Universidad Central de Chile, Santiago 8330507, Chileα-glucosidase is responsible for the hydrolysis of complex carbohydrates into simple absorbable glucose and causes postprandial hyperglycemia. α-glucosidase inhibition is thus the ideal target to prevent postprandial hyperglycemia. The present study was therefore designed to analyze the effects of various compounds isolated from <i>Dryopteris cycadina</i> against α-glucosidase including β-Sitosterol <b>1</b>, β-Sitosterol3-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucopyranoside <b>2</b>, 3, 5, 7-trihydroxy-2-(<i>p</i>-tolyl) chorman-4-one <b>3</b>, Quercetin-3-0-β-<span style="font-variant: small-caps">d</span>-glucopyranoside (3<sup>/</sup>→0-3<sup>///</sup>)- β-<span style="font-variant: small-caps">d</span>- Quercetin -3-0- β –<span style="font-variant: small-caps">d</span>-galactopyranoside <b>4</b> and 5, 7, 4<sup>/</sup>-Trihydroxyflavon-3-glucopyranoid 5. The in vitro spectrophotometric method was used for the analysis of test compounds against possible inhibition. Similarly, molecular docking studies were performed using the MOE software. These compounds showed concentration-dependent inhibition on α-glucosidase, and compounds <b>1</b> (IC<sub>50</sub>: 143 ± 0.47 µM), <b>3</b> (IC<sub>50</sub>:133 ± 6.90 µM) and <b>5</b> (IC<sub>50</sub>: 146 ± 1.93 µM) were more potent than the standard drug, acarbose (IC<sub>50</sub>: 290 ± 0.54 µM). Computational studies of these compounds strongly supported the in vitro studies and showed strong binding receptor sensitivity. In short, the secondary metabolites isolated from <i>D. cycadina</i> demonstrated potent α-glucosidase inhibition that were supported by molecular docking with a high docking score.https://www.mdpi.com/1420-3049/24/3/427<i>Dryopteris cycadina</i>isolated compoundsα-glucosidase inhibitionmolecular docking |
| spellingShingle | Surriya Amin Barkat Ullah Mumtaz Ali Abdur Rauf Haroon Khan Eugenio Uriarte Eduardo Sobarzo-Sánchez Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i> Molecules <i>Dryopteris cycadina</i> isolated compounds α-glucosidase inhibition molecular docking |
| title | Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i> |
| title_full | Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i> |
| title_fullStr | Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i> |
| title_full_unstemmed | Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i> |
| title_short | Potent in Vitro α-Glucosidase Inhibition of Secondary Metabolites Derived from <i>Dryopteris cycadina</i> |
| title_sort | potent in vitro α glucosidase inhibition of secondary metabolites derived from i dryopteris cycadina i |
| topic | <i>Dryopteris cycadina</i> isolated compounds α-glucosidase inhibition molecular docking |
| url | https://www.mdpi.com/1420-3049/24/3/427 |
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