Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes
Aim: The present study was carried out to evaluate the effect of supplementation of β-mercaptoethanol (β-ME) on in vitro maturation rate and glutathione (GSH) level of buffalo oocytes. Materials and Methods: Oocytes were recovered from buffalo’s ovaries collected from government approved slaughter...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2015-02-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.8/February-2015/15.pdf |
| _version_ | 1818442624242024448 |
|---|---|
| author | Pankaj A. Patel Sandhya S. Chaudhary Gopal Puri Virendra Kumar Singh Arjun B. Odedara |
| author_facet | Pankaj A. Patel Sandhya S. Chaudhary Gopal Puri Virendra Kumar Singh Arjun B. Odedara |
| author_sort | Pankaj A. Patel |
| collection | DOAJ |
| description | Aim: The present study was carried out to evaluate the effect of supplementation of β-mercaptoethanol (β-ME) on in vitro maturation rate and glutathione (GSH) level of buffalo oocytes.
Materials and Methods: Oocytes were recovered from buffalo’s ovaries collected from government approved slaughter house (near Kamela darwaza, Surat) of Surat Municipal Corporation. The obtained oocytes were in vitro matured in maturation media supplemented with 0 μM (117 oocytes), 100 μM (46 oocytes) and 200 μM (42 oocytes) concentration of β-ME. After 24 h of incubation, maturation rate of oocytes and intra-cellular GSH level were determined.
Results: The results showed that the presence of β-ME did not influence (p>0.05) the oocyte maturation rate. However, GSH level increased significantly (p<0.05) in matured oocytes when supplemented with 100 μM and 200 μM β-ME (6.19±0.10 and 6.37±0.20 pmol/oocyte) as compared to control media (4.68±0.26 pmol/oocyte).
Conclusion: It was concluded that β-ME may have a potential to increase the meiotic maturation of in vitro cultured oocytes and protect it from oxidative damage. |
| first_indexed | 2024-12-14T18:47:06Z |
| format | Article |
| id | doaj.art-69b98056e96c4b039d0c5e1ad08d8ba8 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-14T18:47:06Z |
| publishDate | 2015-02-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-69b98056e96c4b039d0c5e1ad08d8ba82022-12-21T22:51:21ZengVeterinary WorldVeterinary World0972-89882231-09162015-02-018221321610.14202/vetworld.2015.213-216Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytesPankaj A. Patel0Sandhya S. Chaudhary1Gopal Puri2Virendra Kumar Singh 3Arjun B. Odedara4Department of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India; gamitpankaj09@gmail.comDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India; sandhyachaudhary6@gmail.comDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India; drgopalpuri@gmail.comDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India; drvksingh1981@gmail.comDepartment of Veterinary Physiology & Biochemistry, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Navsari-396450, Gujarat, India; arjunodedra81@gmail.comAim: The present study was carried out to evaluate the effect of supplementation of β-mercaptoethanol (β-ME) on in vitro maturation rate and glutathione (GSH) level of buffalo oocytes. Materials and Methods: Oocytes were recovered from buffalo’s ovaries collected from government approved slaughter house (near Kamela darwaza, Surat) of Surat Municipal Corporation. The obtained oocytes were in vitro matured in maturation media supplemented with 0 μM (117 oocytes), 100 μM (46 oocytes) and 200 μM (42 oocytes) concentration of β-ME. After 24 h of incubation, maturation rate of oocytes and intra-cellular GSH level were determined. Results: The results showed that the presence of β-ME did not influence (p>0.05) the oocyte maturation rate. However, GSH level increased significantly (p<0.05) in matured oocytes when supplemented with 100 μM and 200 μM β-ME (6.19±0.10 and 6.37±0.20 pmol/oocyte) as compared to control media (4.68±0.26 pmol/oocyte). Conclusion: It was concluded that β-ME may have a potential to increase the meiotic maturation of in vitro cultured oocytes and protect it from oxidative damage.http://www.veterinaryworld.org/Vol.8/February-2015/15.pdfbuffalo oocytesin vitro maturationβ-mercaptoethanolglutathione |
| spellingShingle | Pankaj A. Patel Sandhya S. Chaudhary Gopal Puri Virendra Kumar Singh Arjun B. Odedara Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes Veterinary World buffalo oocytes in vitro maturation β-mercaptoethanol glutathione |
| title | Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes |
| title_full | Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes |
| title_fullStr | Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes |
| title_full_unstemmed | Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes |
| title_short | Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes |
| title_sort | effects of β mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes |
| topic | buffalo oocytes in vitro maturation β-mercaptoethanol glutathione |
| url | http://www.veterinaryworld.org/Vol.8/February-2015/15.pdf |
| work_keys_str_mv | AT pankajapatel effectsofbmercaptoethanoloninvitromaturationandglutathionelevelofbuffalooocytes AT sandhyaschaudhary effectsofbmercaptoethanoloninvitromaturationandglutathionelevelofbuffalooocytes AT gopalpuri effectsofbmercaptoethanoloninvitromaturationandglutathionelevelofbuffalooocytes AT virendrakumarsingh effectsofbmercaptoethanoloninvitromaturationandglutathionelevelofbuffalooocytes AT arjunbodedara effectsofbmercaptoethanoloninvitromaturationandglutathionelevelofbuffalooocytes |