Gut Microbiota of Healthy and Malnourished Children in Bangladesh
Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnouri...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2011-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00228/full |
| _version_ | 1811243322861158400 |
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| author | Shirajum eMonira Shota eNakamura Kazuyoshi eGotoh Kaori eIzutsu Haruo eWatanabe Nur Haque Alam Hubert Ph. Endtz Alejandro eCravioto Sk. Imran Ali Takaaki eNakaya Toshihiro eHorii Tetsuya eIida Munirul eAlam |
| author_facet | Shirajum eMonira Shota eNakamura Kazuyoshi eGotoh Kaori eIzutsu Haruo eWatanabe Nur Haque Alam Hubert Ph. Endtz Alejandro eCravioto Sk. Imran Ali Takaaki eNakaya Toshihiro eHorii Tetsuya eIida Munirul eAlam |
| author_sort | Shirajum eMonira |
| collection | DOAJ |
| description | Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnourished children. DNA was extracted from fecal samples of seven healthy and seven malnourished children (n=14; age 2 - 3 years) were analyzed for the variable region of 16S rRNA genes by universal primer PCR followed by high-throughput 454 parallel sequencing to identify the bacterial phyla and genera. Our results reveal that the healthy children had a significantly higher number of OTU in their gut than that of the malnourished children (healthy vs. malnourished: 546 vs. 310). In malnourished children, bacterial population of the phyla Proteobacteria and Bacteroidetes accounted for 46% and 18%, respectively. Conversely, in healthy children, Proteobacteria and Bacteroidetes accounted for 5% and 44%, respectively (P<0.001). In malnourished children, the phylum Proteobacteria included pathogenic genera, namely Klebsiella and Escherichia, which were 174-fold and 9-fold higher, respectively, than their healthy counterpart. The predominance of potentially pathogenic Proteobacteria and minimal level of Bacteroidetes as commensal microbiota might be associated to the ill health of malnourished children in Bangladesh. |
| first_indexed | 2024-04-12T14:05:26Z |
| format | Article |
| id | doaj.art-69ba4bff973849fc8d7380f85661f0b8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T14:05:26Z |
| publishDate | 2011-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-69ba4bff973849fc8d7380f85661f0b82022-12-22T03:30:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2011-11-01210.3389/fmicb.2011.0022814754Gut Microbiota of Healthy and Malnourished Children in BangladeshShirajum eMonira0Shota eNakamura1Kazuyoshi eGotoh2Kaori eIzutsu3Haruo eWatanabe4Nur Haque Alam5Hubert Ph. Endtz6Alejandro eCravioto7Sk. Imran Ali8Takaaki eNakaya9Toshihiro eHorii10Tetsuya eIida11Munirul eAlam12International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.Research Institute for Microbial Diseases, Osaka University, JapanResearch Institute for Microbial Diseases, Osaka University, JapanResearch Institute for Microbial Diseases, Osaka University, JapanNational Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.International Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.Research Institute for Microbial Diseases, Osaka University, JapanResearch Institute for Microbial Diseases, Osaka University, JapanResearch Institute for Microbial Diseases, Osaka University, JapanInternational Center for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnourished children. DNA was extracted from fecal samples of seven healthy and seven malnourished children (n=14; age 2 - 3 years) were analyzed for the variable region of 16S rRNA genes by universal primer PCR followed by high-throughput 454 parallel sequencing to identify the bacterial phyla and genera. Our results reveal that the healthy children had a significantly higher number of OTU in their gut than that of the malnourished children (healthy vs. malnourished: 546 vs. 310). In malnourished children, bacterial population of the phyla Proteobacteria and Bacteroidetes accounted for 46% and 18%, respectively. Conversely, in healthy children, Proteobacteria and Bacteroidetes accounted for 5% and 44%, respectively (P<0.001). In malnourished children, the phylum Proteobacteria included pathogenic genera, namely Klebsiella and Escherichia, which were 174-fold and 9-fold higher, respectively, than their healthy counterpart. The predominance of potentially pathogenic Proteobacteria and minimal level of Bacteroidetes as commensal microbiota might be associated to the ill health of malnourished children in Bangladesh.http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00228/fullmicrobiotaChildrengutnutrition16S rDNA |
| spellingShingle | Shirajum eMonira Shota eNakamura Kazuyoshi eGotoh Kaori eIzutsu Haruo eWatanabe Nur Haque Alam Hubert Ph. Endtz Alejandro eCravioto Sk. Imran Ali Takaaki eNakaya Toshihiro eHorii Tetsuya eIida Munirul eAlam Gut Microbiota of Healthy and Malnourished Children in Bangladesh Frontiers in Microbiology microbiota Children gut nutrition 16S rDNA |
| title | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
| title_full | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
| title_fullStr | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
| title_full_unstemmed | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
| title_short | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
| title_sort | gut microbiota of healthy and malnourished children in bangladesh |
| topic | microbiota Children gut nutrition 16S rDNA |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00228/full |
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