Combined clarification and affinity capture using magnetic resin enables efficient separation of rAAV5 from cell lysate

Combined clarification and affinity capture using magnetic resin enables efficient separation of rAAV5 from cell lysate

Recombinant adeno-associated virus (rAAV) vectors have displayed enormous potential as a platform for delivery of gene therapies. Purification of rAAV at industrial scale involves a series of elaborate, material, and time-consuming midstream steps, such as clarification by depth filtration and conce...

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Bibliographic Details
Main Authors: Federico Turco, Adam Wegelius, Ola Lind, Nils Norrman, Ann-Christin Magnusson, Christine Sund-Lundström, Björn Norén, Jesper Hedberg, Ronnie Palmgren
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
AAV
gene therapy
affinity chromatography
magnetic separation
bioprocessing
process development
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050123001122

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http://www.sciencedirect.com/science/article/pii/S2329050123001122

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