Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection
Aims: This study aimed to explore the diagnostic value of synovial fluid neutrophil extracellular traps (SF-NETs) in periprosthetic joint infection (PJI) diagnosis, and compare it with that of microbial culture, serum ESR and CRP, synovial white blood cell (WBC) count, and polymorphonuclear neutroph...
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| Format: | Article |
| Language: | English |
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The British Editorial Society of Bone & Joint Surgery
2023-02-01
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| Series: | Bone & Joint Research |
| Subjects: | |
| Online Access: | https://online.boneandjoint.org.uk/doi/epdf/10.1302/2046-3758.122.BJR-2022-0391.R1 |
| _version_ | 1797895408068853760 |
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| author | Yuanqing Cai Jialin Liang Xiaoqing Chen Guangyang Zhang Zhaopu Jing Rupeng Zhang Leifeng Lv Wenming Zhang Xiaoqian Dang |
| author_facet | Yuanqing Cai Jialin Liang Xiaoqing Chen Guangyang Zhang Zhaopu Jing Rupeng Zhang Leifeng Lv Wenming Zhang Xiaoqian Dang |
| author_sort | Yuanqing Cai |
| collection | DOAJ |
| description | Aims: This study aimed to explore the diagnostic value of synovial fluid neutrophil extracellular traps (SF-NETs) in periprosthetic joint infection (PJI) diagnosis, and compare it with that of microbial culture, serum ESR and CRP, synovial white blood cell (WBC) count, and polymorphonuclear neutrophil percentage (PMN%). Methods: In a single health centre, patients with suspected PJI were enrolled from January 2013 to December 2021. The inclusion criteria were: 1) patients who were suspected to have PJI; 2) patients with complete medical records; and 3) patients from whom sufficient synovial fluid was obtained for microbial culture and NET test. Patients who received revision surgeries due to aseptic failure (AF) were selected as controls. Synovial fluid was collected for microbial culture and SF-WBC, SF-PNM%, and SF-NET detection. The receiver operating characteristic curve (ROC) of synovial NET, WBC, PMN%, and area under the curve (AUC) were obtained; the diagnostic efficacies of these diagnostic indexes were calculated and compared. Results: The levels of SF-NETs in the PJI group were significantly higher than those of the AF group. The AUC of SF-NET was 0.971 (95% confidence interval (CI) 0.903 to 0.996), the sensitivity was 93.48% (95% CI 82.10% to 98.63%), the specificity was 96.43% (95% CI 81.65% to 99.91%), the accuracy was 94.60% (95% CI 86.73% to 98.50%), the positive predictive value was 97.73%, and the negative predictive value was 90%. Further analysis showed that SF-NET could improve the diagnosis of culture-negative PJI, patients with PJI who received antibiotic treatment preoperatively, and fungal PJI. Conclusion: SF-NET is a novel and ideal synovial fluid biomarker for PJI diagnosis, which could improve PJI diagnosis greatly. Cite this article: Bone Joint Res 2023;12(2):113–120. |
| first_indexed | 2024-04-10T07:25:07Z |
| format | Article |
| id | doaj.art-69c99c1d664e4c9c8bc6d0dc01572e4e |
| institution | Directory Open Access Journal |
| issn | 2046-3758 |
| language | English |
| last_indexed | 2024-04-10T07:25:07Z |
| publishDate | 2023-02-01 |
| publisher | The British Editorial Society of Bone & Joint Surgery |
| record_format | Article |
| series | Bone & Joint Research |
| spelling | doaj.art-69c99c1d664e4c9c8bc6d0dc01572e4e2023-02-24T07:27:22ZengThe British Editorial Society of Bone & Joint SurgeryBone & Joint Research2046-37582023-02-0112211312010.1302/2046-3758.122.BJR-2022-0391.R1Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infectionYuanqing Cai0Jialin Liang1Xiaoqing Chen2Guangyang Zhang3Zhaopu Jing4Rupeng Zhang5Leifeng Lv6Wenming Zhang7Xiaoqian Dang8Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, ChinaDepartment of Orthopaedics, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, ChinaDepartment of Orthopaedics, First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi'an, ChinaAims: This study aimed to explore the diagnostic value of synovial fluid neutrophil extracellular traps (SF-NETs) in periprosthetic joint infection (PJI) diagnosis, and compare it with that of microbial culture, serum ESR and CRP, synovial white blood cell (WBC) count, and polymorphonuclear neutrophil percentage (PMN%). Methods: In a single health centre, patients with suspected PJI were enrolled from January 2013 to December 2021. The inclusion criteria were: 1) patients who were suspected to have PJI; 2) patients with complete medical records; and 3) patients from whom sufficient synovial fluid was obtained for microbial culture and NET test. Patients who received revision surgeries due to aseptic failure (AF) were selected as controls. Synovial fluid was collected for microbial culture and SF-WBC, SF-PNM%, and SF-NET detection. The receiver operating characteristic curve (ROC) of synovial NET, WBC, PMN%, and area under the curve (AUC) were obtained; the diagnostic efficacies of these diagnostic indexes were calculated and compared. Results: The levels of SF-NETs in the PJI group were significantly higher than those of the AF group. The AUC of SF-NET was 0.971 (95% confidence interval (CI) 0.903 to 0.996), the sensitivity was 93.48% (95% CI 82.10% to 98.63%), the specificity was 96.43% (95% CI 81.65% to 99.91%), the accuracy was 94.60% (95% CI 86.73% to 98.50%), the positive predictive value was 97.73%, and the negative predictive value was 90%. Further analysis showed that SF-NET could improve the diagnosis of culture-negative PJI, patients with PJI who received antibiotic treatment preoperatively, and fungal PJI. Conclusion: SF-NET is a novel and ideal synovial fluid biomarker for PJI diagnosis, which could improve PJI diagnosis greatly. Cite this article: Bone Joint Res 2023;12(2):113–120.https://online.boneandjoint.org.uk/doi/epdf/10.1302/2046-3758.122.BJR-2022-0391.R1periprosthetic joint infectionneutrophil extracellular trapsdiagnosissynovial fluidperiprosthetic joint infection (pji)biomarkersrevision surgeriesantibioticscrpwhite blood cellaseptic failureserummann-whitney u test |
| spellingShingle | Yuanqing Cai Jialin Liang Xiaoqing Chen Guangyang Zhang Zhaopu Jing Rupeng Zhang Leifeng Lv Wenming Zhang Xiaoqian Dang Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection Bone & Joint Research periprosthetic joint infection neutrophil extracellular traps diagnosis synovial fluid periprosthetic joint infection (pji) biomarkers revision surgeries antibiotics crp white blood cell aseptic failure serum mann-whitney u test |
| title | Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection |
| title_full | Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection |
| title_fullStr | Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection |
| title_full_unstemmed | Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection |
| title_short | Synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection |
| title_sort | synovial fluid neutrophil extracellular traps could improve the diagnosis of periprosthetic joint infection |
| topic | periprosthetic joint infection neutrophil extracellular traps diagnosis synovial fluid periprosthetic joint infection (pji) biomarkers revision surgeries antibiotics crp white blood cell aseptic failure serum mann-whitney u test |
| url | https://online.boneandjoint.org.uk/doi/epdf/10.1302/2046-3758.122.BJR-2022-0391.R1 |
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