Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization
Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of diff...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/3/401 |
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| author | Abdullah O. Khan Alessandro Di Maio Emily J. Guggenheim Andrew J. Chetwynd Dan Pencross Selina Tang Marie-France A. Belinga-Desaunay Steven G. Thomas Joshua Z. Rappoport Iseult Lynch |
| author_facet | Abdullah O. Khan Alessandro Di Maio Emily J. Guggenheim Andrew J. Chetwynd Dan Pencross Selina Tang Marie-France A. Belinga-Desaunay Steven G. Thomas Joshua Z. Rappoport Iseult Lynch |
| author_sort | Abdullah O. Khan |
| collection | DOAJ |
| description | Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO<sub>2</sub> NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging approaches with an increasing spatial resolution, including automated high throughput live cell imaging, correlative confocal fluorescence and reflectance microscopy, and dSTORM super-resolution microscopy, are used to explore the cellular fate of these NMs and their associated serum proteins. Uncoated TiO<sub>2</sub> NMs demonstrate a rapid loss of corona proteins, while surface coating results in the retention of the corona signal after internalization for at least 24 h (varying with coating composition). Imaging with two-color super-resolution dSTORM revealed that the apparent TiO<sub>2</sub> NM single agglomerates observed in diffraction-limited confocal microscopy are actually adjacent smaller agglomerates, and provides novel insights into the spatial arrangement of the initial and exchanged coronas adsorbed at the NM surfaces. |
| first_indexed | 2024-12-22T21:57:26Z |
| format | Article |
| id | doaj.art-69cb8c400db94ae7b1de5f8f0ea25d55 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-22T21:57:26Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-69cb8c400db94ae7b1de5f8f0ea25d552022-12-21T18:11:13ZengMDPI AGNanomaterials2079-49912020-02-0110340110.3390/nano10030401nano10030401Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular LocalizationAbdullah O. Khan0Alessandro Di Maio1Emily J. Guggenheim2Andrew J. Chetwynd3Dan Pencross4Selina Tang5Marie-France A. Belinga-Desaunay6Steven G. Thomas7Joshua Z. Rappoport8Iseult Lynch9Institute of Cardiovascular Science, College of Medical Dental Sciences, University of Birmingham, Birmingham B15 2TT, UKSchool of Biosciences, University of Birmingham, Birmingham B15 2TT, UKSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UKSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UKInstitute of Cardiovascular Science, College of Medical Dental Sciences, University of Birmingham, Birmingham B15 2TT, UKPromethean Particles Ltd., 1-3 Genesis Park, Midland Way, Nottingham NG7 3EF, UKSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UKInstitute of Cardiovascular Science, College of Medical Dental Sciences, University of Birmingham, Birmingham B15 2TT, UKBoston College, Higgins 644A, 140 Commonwealth Ave, Chestnut Hill, MA 02467, USASchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UKNanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO<sub>2</sub> NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging approaches with an increasing spatial resolution, including automated high throughput live cell imaging, correlative confocal fluorescence and reflectance microscopy, and dSTORM super-resolution microscopy, are used to explore the cellular fate of these NMs and their associated serum proteins. Uncoated TiO<sub>2</sub> NMs demonstrate a rapid loss of corona proteins, while surface coating results in the retention of the corona signal after internalization for at least 24 h (varying with coating composition). Imaging with two-color super-resolution dSTORM revealed that the apparent TiO<sub>2</sub> NM single agglomerates observed in diffraction-limited confocal microscopy are actually adjacent smaller agglomerates, and provides novel insights into the spatial arrangement of the initial and exchanged coronas adsorbed at the NM surfaces.https://www.mdpi.com/2079-4991/10/3/401nanosafetyprotein coronabionano-interfacecellular uptakecellular localizationco-localisationreflectance imaging |
| spellingShingle | Abdullah O. Khan Alessandro Di Maio Emily J. Guggenheim Andrew J. Chetwynd Dan Pencross Selina Tang Marie-France A. Belinga-Desaunay Steven G. Thomas Joshua Z. Rappoport Iseult Lynch Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization Nanomaterials nanosafety protein corona bionano-interface cellular uptake cellular localization co-localisation reflectance imaging |
| title | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization |
| title_full | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization |
| title_fullStr | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization |
| title_full_unstemmed | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization |
| title_short | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization |
| title_sort | surface chemistry dependent evolution of the nanomaterial corona on tio sub 2 sub nanomaterials following uptake and sub cellular localization |
| topic | nanosafety protein corona bionano-interface cellular uptake cellular localization co-localisation reflectance imaging |
| url | https://www.mdpi.com/2079-4991/10/3/401 |
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