Essential role for SUN5 in anchoring sperm head to the tail
SUN (Sad1 and UNC84 domain containing)-domain proteins are reported to reside on the nuclear membrane playing distinct roles in nuclear dynamics. SUN5 is a new member of the SUN family, with little knowledge regarding its function. Here, we generated Sun5−/− mice and found that male mice were infert...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/28199 |
| _version_ | 1811181089866121216 |
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| author | Yongliang Shang Fuxi Zhu Lina Wang Ying-Chun Ouyang Ming-Zhe Dong Chao Liu Haichao Zhao Xiuhong Cui Dongyuan Ma Zhiguo Zhang Xiaoyu Yang Yueshuai Guo Feng Liu Li Yuan Fei Gao Xuejiang Guo Qing-Yuan Sun Yunxia Cao Wei Li |
| author_facet | Yongliang Shang Fuxi Zhu Lina Wang Ying-Chun Ouyang Ming-Zhe Dong Chao Liu Haichao Zhao Xiuhong Cui Dongyuan Ma Zhiguo Zhang Xiaoyu Yang Yueshuai Guo Feng Liu Li Yuan Fei Gao Xuejiang Guo Qing-Yuan Sun Yunxia Cao Wei Li |
| author_sort | Yongliang Shang |
| collection | DOAJ |
| description | SUN (Sad1 and UNC84 domain containing)-domain proteins are reported to reside on the nuclear membrane playing distinct roles in nuclear dynamics. SUN5 is a new member of the SUN family, with little knowledge regarding its function. Here, we generated Sun5−/− mice and found that male mice were infertile. Most Sun5-null spermatozoa displayed a globozoospermia-like phenotype but they were actually acephalic spermatozoa. Additional studies revealed that SUN5 was located in the neck of the spermatozoa, anchoring sperm head to the tail, and without functional SUN5 the sperm head to tail coupling apparatus was detached from nucleus during spermatid elongation. Finally, we found that healthy heterozygous offspring could be obtained via intracytoplasmic injection of Sun5-mutated sperm heads for both male mice and patients. Our studies reveal the essential role of SUN5 in anchoring sperm head to the tail and provide a promising way to treat this kind of acephalic spermatozoa-associated male infertility. |
| first_indexed | 2024-04-11T09:12:35Z |
| format | Article |
| id | doaj.art-69cbd51f21454f31b2adf8f2316beff1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:12:35Z |
| publishDate | 2017-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-69cbd51f21454f31b2adf8f2316beff12022-12-22T04:32:26ZengeLife Sciences Publications LtdeLife2050-084X2017-09-01610.7554/eLife.28199Essential role for SUN5 in anchoring sperm head to the tailYongliang Shang0Fuxi Zhu1Lina Wang2Ying-Chun Ouyang3Ming-Zhe Dong4Chao Liu5Haichao Zhao6Xiuhong Cui7Dongyuan Ma8Zhiguo Zhang9Xiaoyu Yang10Yueshuai Guo11Feng Liu12https://orcid.org/0000-0003-3228-0943Li Yuan13Fei Gao14Xuejiang Guo15Qing-Yuan Sun16Yunxia Cao17Wei Li18https://orcid.org/0000-0002-6235-0749State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, ChinaReproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Institute of Reproductive Genetics, Anhui Medical University, Hefei, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaReproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Institute of Reproductive Genetics, Anhui Medical University, Hefei, ChinaCenter of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaUniversity of Chinese Academy of Sciences, Beijing, China; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaSavaid School of Medicine, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Reproductive Medicine, Collaborative Innovation Center of Genetics and Development, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, ChinaReproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Institute of Reproductive Genetics, Anhui Medical University, Hefei, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, ChinaSUN (Sad1 and UNC84 domain containing)-domain proteins are reported to reside on the nuclear membrane playing distinct roles in nuclear dynamics. SUN5 is a new member of the SUN family, with little knowledge regarding its function. Here, we generated Sun5−/− mice and found that male mice were infertile. Most Sun5-null spermatozoa displayed a globozoospermia-like phenotype but they were actually acephalic spermatozoa. Additional studies revealed that SUN5 was located in the neck of the spermatozoa, anchoring sperm head to the tail, and without functional SUN5 the sperm head to tail coupling apparatus was detached from nucleus during spermatid elongation. Finally, we found that healthy heterozygous offspring could be obtained via intracytoplasmic injection of Sun5-mutated sperm heads for both male mice and patients. Our studies reveal the essential role of SUN5 in anchoring sperm head to the tail and provide a promising way to treat this kind of acephalic spermatozoa-associated male infertility.https://elifesciences.org/articles/28199SUN5ICSIsperm head-tail connectionacephalic spermatozoapseudo-globozoospermia |
| spellingShingle | Yongliang Shang Fuxi Zhu Lina Wang Ying-Chun Ouyang Ming-Zhe Dong Chao Liu Haichao Zhao Xiuhong Cui Dongyuan Ma Zhiguo Zhang Xiaoyu Yang Yueshuai Guo Feng Liu Li Yuan Fei Gao Xuejiang Guo Qing-Yuan Sun Yunxia Cao Wei Li Essential role for SUN5 in anchoring sperm head to the tail eLife SUN5 ICSI sperm head-tail connection acephalic spermatozoa pseudo-globozoospermia |
| title | Essential role for SUN5 in anchoring sperm head to the tail |
| title_full | Essential role for SUN5 in anchoring sperm head to the tail |
| title_fullStr | Essential role for SUN5 in anchoring sperm head to the tail |
| title_full_unstemmed | Essential role for SUN5 in anchoring sperm head to the tail |
| title_short | Essential role for SUN5 in anchoring sperm head to the tail |
| title_sort | essential role for sun5 in anchoring sperm head to the tail |
| topic | SUN5 ICSI sperm head-tail connection acephalic spermatozoa pseudo-globozoospermia |
| url | https://elifesciences.org/articles/28199 |
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