Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) Cultivars
The aim of this study was to evaluate physiological responses to short-term heat stress in the leaves of traditional (Bistrica) and modern (Toptaste) plum cultivars. In this study, detached plum leaves were incubated at 25 °C (control) and 40 °C (stress). After 1 h of exposure to heat (40 °C), chlor...
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MDPI AG
2022-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/1/72 |
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| author | Marija Viljevac Vuletić Ines Mihaljević Vesna Tomaš Daniela Horvat Zvonimir Zdunić Dominik Vuković |
| author_facet | Marija Viljevac Vuletić Ines Mihaljević Vesna Tomaš Daniela Horvat Zvonimir Zdunić Dominik Vuković |
| author_sort | Marija Viljevac Vuletić |
| collection | DOAJ |
| description | The aim of this study was to evaluate physiological responses to short-term heat stress in the leaves of traditional (Bistrica) and modern (Toptaste) plum cultivars. In this study, detached plum leaves were incubated at 25 °C (control) and 40 °C (stress). After 1 h of exposure to heat (40 °C), chlorophyll <i>a</i> fluorescence transients were measured, and several biochemical parameters were analyzed. Elevated temperature caused heat stress in both plum cultivars, seen as a decrease in water content (WT), but in the leaves of the cultivar Bistrica, an accumulation of proline and phenols, as well as an accumulation of photosynthetic pigments, suggest the activation of a significant response to unfavorable conditions. Conversely, in the leaves of Toptaste, a significant accumulation of malondialdehyde (MDA) and an activation of guaiacol peroxidase (GPOD), all together with a decreased soluble proteins content, indicate an inadequate response to maintaining homeostasis in the leaf metabolism. The impact of an elevated temperature on photosynthesis was significant in both plum cultivars as reflected in the decrease in performance indexes (PI<sub>ABS</sub> and PI<sub>total</sub>) and the maximum quantum yield of PSII (F<sub>v</sub>/F<sub>m</sub>), with significantly pronounced changes found in Toptaste. Unlike the traditional plum cultivar, Bistrica, in the modern cultivar, Toptaste, short-term heat stress increased the minimal fluorescence (F<sub>0</sub>) and absorption (ABS/RC), as well as Chl <i>b</i> in total chlorophylls. Additionally, the inactivation of RCs (RC/ABS) suggests that excitation energy was not trapped efficiently in the electron chain transport, which resulted in stronger dissipation (DI<sub>0</sub>/RC) and the formation of ROSs. Considering all presented results, it can be presumed that the traditional cultivar Bistrica has better tolerance to heat stress than the modern cultivar Toptaste. The cultivar, Bistrica, can be used as a basis in further plum breeding programs, as a source of tolerance for high temperature stress. |
| first_indexed | 2024-03-10T01:21:55Z |
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| id | doaj.art-69d28848459d4caeaf8430f6a4403d8d |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T01:21:55Z |
| publishDate | 2022-01-01 |
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| series | Horticulturae |
| spelling | doaj.art-69d28848459d4caeaf8430f6a4403d8d2023-11-23T13:58:09ZengMDPI AGHorticulturae2311-75242022-01-01817210.3390/horticulturae8010072Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) CultivarsMarija Viljevac Vuletić0Ines Mihaljević1Vesna Tomaš2Daniela Horvat3Zvonimir Zdunić4Dominik Vuković5Agricultural Institute Osijek, Južno Predgrađe 17, 31000 Osijek, CroatiaAgricultural Institute Osijek, Južno Predgrađe 17, 31000 Osijek, CroatiaAgricultural Institute Osijek, Južno Predgrađe 17, 31000 Osijek, CroatiaAgricultural Institute Osijek, Južno Predgrađe 17, 31000 Osijek, CroatiaAgricultural Institute Osijek, Južno Predgrađe 17, 31000 Osijek, CroatiaAgricultural Institute Osijek, Južno Predgrađe 17, 31000 Osijek, CroatiaThe aim of this study was to evaluate physiological responses to short-term heat stress in the leaves of traditional (Bistrica) and modern (Toptaste) plum cultivars. In this study, detached plum leaves were incubated at 25 °C (control) and 40 °C (stress). After 1 h of exposure to heat (40 °C), chlorophyll <i>a</i> fluorescence transients were measured, and several biochemical parameters were analyzed. Elevated temperature caused heat stress in both plum cultivars, seen as a decrease in water content (WT), but in the leaves of the cultivar Bistrica, an accumulation of proline and phenols, as well as an accumulation of photosynthetic pigments, suggest the activation of a significant response to unfavorable conditions. Conversely, in the leaves of Toptaste, a significant accumulation of malondialdehyde (MDA) and an activation of guaiacol peroxidase (GPOD), all together with a decreased soluble proteins content, indicate an inadequate response to maintaining homeostasis in the leaf metabolism. The impact of an elevated temperature on photosynthesis was significant in both plum cultivars as reflected in the decrease in performance indexes (PI<sub>ABS</sub> and PI<sub>total</sub>) and the maximum quantum yield of PSII (F<sub>v</sub>/F<sub>m</sub>), with significantly pronounced changes found in Toptaste. Unlike the traditional plum cultivar, Bistrica, in the modern cultivar, Toptaste, short-term heat stress increased the minimal fluorescence (F<sub>0</sub>) and absorption (ABS/RC), as well as Chl <i>b</i> in total chlorophylls. Additionally, the inactivation of RCs (RC/ABS) suggests that excitation energy was not trapped efficiently in the electron chain transport, which resulted in stronger dissipation (DI<sub>0</sub>/RC) and the formation of ROSs. Considering all presented results, it can be presumed that the traditional cultivar Bistrica has better tolerance to heat stress than the modern cultivar Toptaste. The cultivar, Bistrica, can be used as a basis in further plum breeding programs, as a source of tolerance for high temperature stress.https://www.mdpi.com/2311-7524/8/1/72antioxidative responseautochthonouschlorophyll fluorescenceheat toleranceJIP testphotosynthesis |
| spellingShingle | Marija Viljevac Vuletić Ines Mihaljević Vesna Tomaš Daniela Horvat Zvonimir Zdunić Dominik Vuković Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) Cultivars Horticulturae antioxidative response autochthonous chlorophyll fluorescence heat tolerance JIP test photosynthesis |
| title | Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) Cultivars |
| title_full | Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) Cultivars |
| title_fullStr | Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) Cultivars |
| title_full_unstemmed | Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) Cultivars |
| title_short | Physiological Response to Short-Term Heat Stress in the Leaves of Traditional and Modern Plum (<i>Prunus domestica</i> L.) Cultivars |
| title_sort | physiological response to short term heat stress in the leaves of traditional and modern plum i prunus domestica i l cultivars |
| topic | antioxidative response autochthonous chlorophyll fluorescence heat tolerance JIP test photosynthesis |
| url | https://www.mdpi.com/2311-7524/8/1/72 |
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