Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro Study

The aim of this study is to evaluate salivary remineralisation versus chemical remineralisation/infiltration of enamel, using different dentistry materials. The enamel changes were studied using confocal laser scanning microscopy (CLSM), and the depth of lesions and demineralisation/remineralisation...

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Main Authors: Lia-Raluca Damian, Ramona Dumitrescu, Vlad Tiberiu Alexa, David Focht, Cristoph Schwartz, Octavia Balean, Daniela Jumanca, Diana Obistioiu, Dacian Lalescu, Sebastian-Aurelian Stefaniga, Adina Berbecea, Aurora Doris Fratila, Alexandra Denisa Scurtu, Atena Galuscan
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Materials
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Online Access:https://www.mdpi.com/1996-1944/15/20/7258
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author Lia-Raluca Damian
Ramona Dumitrescu
Vlad Tiberiu Alexa
David Focht
Cristoph Schwartz
Octavia Balean
Daniela Jumanca
Diana Obistioiu
Dacian Lalescu
Sebastian-Aurelian Stefaniga
Adina Berbecea
Aurora Doris Fratila
Alexandra Denisa Scurtu
Atena Galuscan
author_facet Lia-Raluca Damian
Ramona Dumitrescu
Vlad Tiberiu Alexa
David Focht
Cristoph Schwartz
Octavia Balean
Daniela Jumanca
Diana Obistioiu
Dacian Lalescu
Sebastian-Aurelian Stefaniga
Adina Berbecea
Aurora Doris Fratila
Alexandra Denisa Scurtu
Atena Galuscan
author_sort Lia-Raluca Damian
collection DOAJ
description The aim of this study is to evaluate salivary remineralisation versus chemical remineralisation/infiltration of enamel, using different dentistry materials. The enamel changes were studied using confocal laser scanning microscopy (CLSM), and the depth of lesions and demineralisation/remineralisation/infiltration percentage were calculated. Additionally, the macro elemental composition of the teeth was performed using atomic absorption spectroscopy (AAS). Two studies were performed: (i) demineralisation of enamel in 3% citric acid and infiltration treatment with infiltration resin (Icon, DMG), remineralisation with Fluor Protector (Ivoclar Vivadent) and artificial saliva pH 8; and (ii) enamel demineralisation in saliva at pH 3 and remineralisation at salivary pH 8. The results showed that, firstly, for the remineralisation of demineralised enamel samples, Fluor Protector (Ivoclar Vivadent) was very effective for medium demineralised lesions followed by saliva remineralisation. In cases of deep demineralisation lesions where fluoride could not penetrate, low viscosity resin (Icon, DMG, Hamburg) effectively infiltrated to stop the demineralisation process. Secondly, remineralisation in salivary conditions needed supplementary study over a longer period, to analyse the habits, diet and nutrition of patients in detail. Finally, demineralisation/remineralisation processes were found to influence the macro elemental composition of enamel demineralisation, with natural saliva proving to be less aggressive in terms of decreasing Ca and Mg content.
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spelling doaj.art-69d2aa1075d5409a8f64ab872415ed052023-11-24T01:04:48ZengMDPI AGMaterials1996-19442022-10-011520725810.3390/ma15207258Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro StudyLia-Raluca Damian0Ramona Dumitrescu1Vlad Tiberiu Alexa2David Focht3Cristoph Schwartz4Octavia Balean5Daniela Jumanca6Diana Obistioiu7Dacian Lalescu8Sebastian-Aurelian Stefaniga9Adina Berbecea10Aurora Doris Fratila11Alexandra Denisa Scurtu12Atena Galuscan13Faculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timișoara, Calea Aradului No. 119, 300645 Timisoara, RomaniaFaculty of Food Engineering, University of Life Sciences “King Michael I” from Timișoara, Calea Aradului No. 119, 300645 Timisoara, RomaniaComputer Science Department, West University of Timisoara, UVT, 300223 Timisoara, RomaniaFaculty of Agriculture, University of Life Sciences “King Michael I” from Timișoara, Calea Aradului No. 119, 300641 Timisoara, RomaniaFaculty of Dental Medicine, Ludwig-Maximilian University Munich, Goethestr. 70, 80336 Munich, GermanyFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaFaculty of Dentistry, Department I, University of Medicine and Pharmacy “Victor Babes”, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaThe aim of this study is to evaluate salivary remineralisation versus chemical remineralisation/infiltration of enamel, using different dentistry materials. The enamel changes were studied using confocal laser scanning microscopy (CLSM), and the depth of lesions and demineralisation/remineralisation/infiltration percentage were calculated. Additionally, the macro elemental composition of the teeth was performed using atomic absorption spectroscopy (AAS). Two studies were performed: (i) demineralisation of enamel in 3% citric acid and infiltration treatment with infiltration resin (Icon, DMG), remineralisation with Fluor Protector (Ivoclar Vivadent) and artificial saliva pH 8; and (ii) enamel demineralisation in saliva at pH 3 and remineralisation at salivary pH 8. The results showed that, firstly, for the remineralisation of demineralised enamel samples, Fluor Protector (Ivoclar Vivadent) was very effective for medium demineralised lesions followed by saliva remineralisation. In cases of deep demineralisation lesions where fluoride could not penetrate, low viscosity resin (Icon, DMG, Hamburg) effectively infiltrated to stop the demineralisation process. Secondly, remineralisation in salivary conditions needed supplementary study over a longer period, to analyse the habits, diet and nutrition of patients in detail. Finally, demineralisation/remineralisation processes were found to influence the macro elemental composition of enamel demineralisation, with natural saliva proving to be less aggressive in terms of decreasing Ca and Mg content.https://www.mdpi.com/1996-1944/15/20/7258confocal microscopyremineralisationsalivary pHresin infiltrationFluorprevention dentistry
spellingShingle Lia-Raluca Damian
Ramona Dumitrescu
Vlad Tiberiu Alexa
David Focht
Cristoph Schwartz
Octavia Balean
Daniela Jumanca
Diana Obistioiu
Dacian Lalescu
Sebastian-Aurelian Stefaniga
Adina Berbecea
Aurora Doris Fratila
Alexandra Denisa Scurtu
Atena Galuscan
Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro Study
Materials
confocal microscopy
remineralisation
salivary pH
resin infiltration
Fluor
prevention dentistry
title Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro Study
title_full Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro Study
title_fullStr Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro Study
title_full_unstemmed Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro Study
title_short Impact of Dentistry Materials on Chemical Remineralisation/Infiltration versus Salivary Remineralisation of Enamel—In Vitro Study
title_sort impact of dentistry materials on chemical remineralisation infiltration versus salivary remineralisation of enamel in vitro study
topic confocal microscopy
remineralisation
salivary pH
resin infiltration
Fluor
prevention dentistry
url https://www.mdpi.com/1996-1944/15/20/7258
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