Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects
Sericin-alginate hydrogel formulations with purple waxy corn (<i>Zea mays</i> L.) cob extract (PWCC) for topical anti-inflammatory application are developed and evaluated. The physical properties such as viscosity, pH, and anthocyanin release are examined and in vitro anti-inflammatory a...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/3/577 |
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| author | Nattawadee Kanpipit Natsajee Nualkaew Worawikunya Kiatponglarp Aroonsri Priprem Suthasinee Thapphasaraphong |
| author_facet | Nattawadee Kanpipit Natsajee Nualkaew Worawikunya Kiatponglarp Aroonsri Priprem Suthasinee Thapphasaraphong |
| author_sort | Nattawadee Kanpipit |
| collection | DOAJ |
| description | Sericin-alginate hydrogel formulations with purple waxy corn (<i>Zea mays</i> L.) cob extract (PWCC) for topical anti-inflammatory application are developed and evaluated. The physical properties such as viscosity, pH, and anthocyanin release are examined and in vitro anti-inflammatory activities, such as NO inhibition and IL-6, IL-1β, TNF-α, iNOS, and COX-2 expression, are evaluated in LPS-stimulated RAW 264.7 murine macrophages. The sericin-alginate hydrogel is prepared by physical crosslinking through the ionic interaction of the polymers combined with anthocyanin from PWCC at pH 6.5. The hydrogel formulation with 2.00% <i>w</i>/<i>v</i> sericin, 0.20% <i>w</i>/<i>v</i> alginate, and 0.15% <i>w</i>/<i>v</i> PWCC (SN6) shows a suitable viscosity for topical treatment, the highest nitric oxide inhibition (79.43%), no cytotoxicity, and reduced expression of IL-6, IL-1β, and TNF-α mediators. Moreover, the SN6 formulation displays a sustained anthocyanin release over 8–12 h, which correlates with the Korsmeyer–Peppas model. The FT-IR spectrum of SN6 confirmed interaction of the sericin polymer with anthocyanins from PWCC via H-bonding by the shifted peak of amide I and amide III. In addition, the anthocyanin is stable in sericin hydrogels under heating-cooling storage conditions. Therefore, we suggest that this hydrogel formulation has potential as an anti-inflammatory agent. The formulation will be further investigated for in vivo studies and clinical trials in the future. |
| first_indexed | 2024-03-09T12:58:49Z |
| format | Article |
| id | doaj.art-69de74f61f484cb19be8e1e92cea928b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T12:58:49Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-69de74f61f484cb19be8e1e92cea928b2023-11-30T21:56:53ZengMDPI AGPharmaceutics1999-49232022-03-0114357710.3390/pharmaceutics14030577Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory EffectsNattawadee Kanpipit0Natsajee Nualkaew1Worawikunya Kiatponglarp2Aroonsri Priprem3Suthasinee Thapphasaraphong4Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandSynchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, ThailandFaculty of Pharmacy, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandSericin-alginate hydrogel formulations with purple waxy corn (<i>Zea mays</i> L.) cob extract (PWCC) for topical anti-inflammatory application are developed and evaluated. The physical properties such as viscosity, pH, and anthocyanin release are examined and in vitro anti-inflammatory activities, such as NO inhibition and IL-6, IL-1β, TNF-α, iNOS, and COX-2 expression, are evaluated in LPS-stimulated RAW 264.7 murine macrophages. The sericin-alginate hydrogel is prepared by physical crosslinking through the ionic interaction of the polymers combined with anthocyanin from PWCC at pH 6.5. The hydrogel formulation with 2.00% <i>w</i>/<i>v</i> sericin, 0.20% <i>w</i>/<i>v</i> alginate, and 0.15% <i>w</i>/<i>v</i> PWCC (SN6) shows a suitable viscosity for topical treatment, the highest nitric oxide inhibition (79.43%), no cytotoxicity, and reduced expression of IL-6, IL-1β, and TNF-α mediators. Moreover, the SN6 formulation displays a sustained anthocyanin release over 8–12 h, which correlates with the Korsmeyer–Peppas model. The FT-IR spectrum of SN6 confirmed interaction of the sericin polymer with anthocyanins from PWCC via H-bonding by the shifted peak of amide I and amide III. In addition, the anthocyanin is stable in sericin hydrogels under heating-cooling storage conditions. Therefore, we suggest that this hydrogel formulation has potential as an anti-inflammatory agent. The formulation will be further investigated for in vivo studies and clinical trials in the future.https://www.mdpi.com/1999-4923/14/3/577sericin-alginate hydrogelsFT-IRcontrolled releaseinflammatory cytokinestopical hydrogels |
| spellingShingle | Nattawadee Kanpipit Natsajee Nualkaew Worawikunya Kiatponglarp Aroonsri Priprem Suthasinee Thapphasaraphong Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects Pharmaceutics sericin-alginate hydrogels FT-IR controlled release inflammatory cytokines topical hydrogels |
| title | Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects |
| title_full | Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects |
| title_fullStr | Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects |
| title_full_unstemmed | Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects |
| title_short | Development of a Sericin Hydrogel to Deliver Anthocyanins from Purple Waxy Corn Cob (<i>Zea mays</i> L.) Extract and In Vitro Evaluation of Anti-Inflammatory Effects |
| title_sort | development of a sericin hydrogel to deliver anthocyanins from purple waxy corn cob i zea mays i l extract and in vitro evaluation of anti inflammatory effects |
| topic | sericin-alginate hydrogels FT-IR controlled release inflammatory cytokines topical hydrogels |
| url | https://www.mdpi.com/1999-4923/14/3/577 |
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