Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless Steel
Biofilm formation by <i>E. coli</i> is a serious threat to meat processing plants. Chemical disinfectants often fail to eliminate biofilms; thus, bacteriophages are a promising alternative to solve this problem, since they are widely distributed, environmentally friendly, and nontoxic to...
| Hlavní autoři: | , , , , , , |
|---|---|
| Médium: | Článek |
| Jazyk: | English |
| Vydáno: |
MDPI AG
2021-09-01
|
| Edice: | Antibiotics |
| Témata: | |
| On-line přístup: | https://www.mdpi.com/2079-6382/10/10/1150 |
| _version_ | 1827680324348280832 |
|---|---|
| author | Jean Pierre González-Gómez Berenice González-Torres Pedro Javier Guerrero-Medina Osvaldo López-Cuevas Cristóbal Chaidez María Guadalupe Avila-Novoa Melesio Gutiérrez-Lomelí |
| author_facet | Jean Pierre González-Gómez Berenice González-Torres Pedro Javier Guerrero-Medina Osvaldo López-Cuevas Cristóbal Chaidez María Guadalupe Avila-Novoa Melesio Gutiérrez-Lomelí |
| author_sort | Jean Pierre González-Gómez |
| collection | DOAJ |
| description | Biofilm formation by <i>E. coli</i> is a serious threat to meat processing plants. Chemical disinfectants often fail to eliminate biofilms; thus, bacteriophages are a promising alternative to solve this problem, since they are widely distributed, environmentally friendly, and nontoxic to humans. In this study, the biofilm formation of 10 <i>E. coli</i> strains isolated from the meat industry and <i>E. coli</i> ATCC BAA-1430 and ATCC 11303 were evaluated. Three strains, isolated from the meat contact surfaces, showed adhesion ability and produced extracellular polymeric substances. Biofilms of these three strains were developed onto stainless steel (SS) surfaces and enumerated at 2, 12, 24, 48, and 120 h, and were visualized by scanning electron microscopy. Subsequently, three bacteriophages showing podovirus morphology were isolated from ground beef and poultry liver samples, which showed lytic activity against the abovementioned biofilm-forming strains. SS surfaces with biofilms of 2, 14, and 48 h maturity were treated with mixed and individual bacteriophages at 8 and 9 log<sub>10</sub> PFU/mL for 1 h. The results showed reductions greater than 6 log<sub>10</sub> CFU/cm<sup>2</sup> as a result of exposing SS surfaces with biofilms of 24 h maturity to 9 log<sub>10</sub> PFU/mL of bacteriophages; however, the <i>E. coli</i> and bacteriophage strains, phage concentration, and biofilm development stage had significant effects on biofilm reduction (<i>p</i> < 0.05). In conclusion, the isolated bacteriophages showed effectiveness at reducing biofilms of isolated <i>E. coli</i>; however, it is necessary to increase the libraries of phages with lytic activity against the strains isolated from production environments. |
| first_indexed | 2024-03-10T06:46:17Z |
| format | Article |
| id | doaj.art-69e005313db74421907d0de6677174bb |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T06:46:17Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-69e005313db74421907d0de6677174bb2023-11-22T17:12:50ZengMDPI AGAntibiotics2079-63822021-09-011010115010.3390/antibiotics10101150Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless SteelJean Pierre González-Gómez0Berenice González-Torres1Pedro Javier Guerrero-Medina2Osvaldo López-Cuevas3Cristóbal Chaidez4María Guadalupe Avila-Novoa5Melesio Gutiérrez-Lomelí6Centro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Ocotlán 47820, MexicoCentro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Ocotlán 47820, MexicoCentro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Ocotlán 47820, MexicoLaboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a Eldorado Km 5.5, Culiacán 80110, MexicoLaboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a Eldorado Km 5.5, Culiacán 80110, MexicoCentro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Ocotlán 47820, MexicoCentro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Ocotlán 47820, MexicoBiofilm formation by <i>E. coli</i> is a serious threat to meat processing plants. Chemical disinfectants often fail to eliminate biofilms; thus, bacteriophages are a promising alternative to solve this problem, since they are widely distributed, environmentally friendly, and nontoxic to humans. In this study, the biofilm formation of 10 <i>E. coli</i> strains isolated from the meat industry and <i>E. coli</i> ATCC BAA-1430 and ATCC 11303 were evaluated. Three strains, isolated from the meat contact surfaces, showed adhesion ability and produced extracellular polymeric substances. Biofilms of these three strains were developed onto stainless steel (SS) surfaces and enumerated at 2, 12, 24, 48, and 120 h, and were visualized by scanning electron microscopy. Subsequently, three bacteriophages showing podovirus morphology were isolated from ground beef and poultry liver samples, which showed lytic activity against the abovementioned biofilm-forming strains. SS surfaces with biofilms of 2, 14, and 48 h maturity were treated with mixed and individual bacteriophages at 8 and 9 log<sub>10</sub> PFU/mL for 1 h. The results showed reductions greater than 6 log<sub>10</sub> CFU/cm<sup>2</sup> as a result of exposing SS surfaces with biofilms of 24 h maturity to 9 log<sub>10</sub> PFU/mL of bacteriophages; however, the <i>E. coli</i> and bacteriophage strains, phage concentration, and biofilm development stage had significant effects on biofilm reduction (<i>p</i> < 0.05). In conclusion, the isolated bacteriophages showed effectiveness at reducing biofilms of isolated <i>E. coli</i>; however, it is necessary to increase the libraries of phages with lytic activity against the strains isolated from production environments.https://www.mdpi.com/2079-6382/10/10/1150<i>E. coli</i> biofilmsfood contact surfacesbiocontrolbacteriophages |
| spellingShingle | Jean Pierre González-Gómez Berenice González-Torres Pedro Javier Guerrero-Medina Osvaldo López-Cuevas Cristóbal Chaidez María Guadalupe Avila-Novoa Melesio Gutiérrez-Lomelí Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless Steel Antibiotics <i>E. coli</i> biofilms food contact surfaces biocontrol bacteriophages |
| title | Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless Steel |
| title_full | Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless Steel |
| title_fullStr | Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless Steel |
| title_full_unstemmed | Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless Steel |
| title_short | Efficacy of Novel Bacteriophages against <i>Escherichia coli</i> Biofilms on Stainless Steel |
| title_sort | efficacy of novel bacteriophages against i escherichia coli i biofilms on stainless steel |
| topic | <i>E. coli</i> biofilms food contact surfaces biocontrol bacteriophages |
| url | https://www.mdpi.com/2079-6382/10/10/1150 |
| work_keys_str_mv | AT jeanpierregonzalezgomez efficacyofnovelbacteriophagesagainstiescherichiacoliibiofilmsonstainlesssteel AT berenicegonzaleztorres efficacyofnovelbacteriophagesagainstiescherichiacoliibiofilmsonstainlesssteel AT pedrojavierguerreromedina efficacyofnovelbacteriophagesagainstiescherichiacoliibiofilmsonstainlesssteel AT osvaldolopezcuevas efficacyofnovelbacteriophagesagainstiescherichiacoliibiofilmsonstainlesssteel AT cristobalchaidez efficacyofnovelbacteriophagesagainstiescherichiacoliibiofilmsonstainlesssteel AT mariaguadalupeavilanovoa efficacyofnovelbacteriophagesagainstiescherichiacoliibiofilmsonstainlesssteel AT melesiogutierrezlomeli efficacyofnovelbacteriophagesagainstiescherichiacoliibiofilmsonstainlesssteel |