Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals
Abstract Background Our previous study indicated that emergency contraception, including levonorgestrel and progesterone, could lead to ectopic pregnancy following contraception failure. However, our understanding of the effects of levonorgestrel and progesterone on oviductal physiology is limited....
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12958-018-0377-3 |
| _version_ | 1819157047391813632 |
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| author | Cheng Li Hui-Yu Zhang Yan Liang Wei Xia Qian Zhu Duo Zhang Zhen Huang Gui-Lin Liang Rui-Hong Xue Hang Qi Xiao-Qing He Jiang-Jing Yuan Ya-Jing Tan He-Feng Huang Jian Zhang |
| author_facet | Cheng Li Hui-Yu Zhang Yan Liang Wei Xia Qian Zhu Duo Zhang Zhen Huang Gui-Lin Liang Rui-Hong Xue Hang Qi Xiao-Qing He Jiang-Jing Yuan Ya-Jing Tan He-Feng Huang Jian Zhang |
| author_sort | Cheng Li |
| collection | DOAJ |
| description | Abstract Background Our previous study indicated that emergency contraception, including levonorgestrel and progesterone, could lead to ectopic pregnancy following contraception failure. However, our understanding of the effects of levonorgestrel and progesterone on oviductal physiology is limited. Methods The receptivity of the fallopian tubal epithelium after levonorgestrel and progesterone treatment was examined through western blots for receptivity markers and JAr-spheroid-fallopian tubal epithelial cell attachment assays. The ciliary beat frequency was analyzed using an inverted bright-field microscope. Furthermore, an in vivo animal model of embryo-tubal transplantation was also studied to determine the effects of levonorgestrel- and progesterone-induced ciliary beat reduction. Results Our results showed that levonorgestrel and progesterone did not change the levels of fallopian tubal epithelial cell receptive markers, including LIF, STAT3, IGFBP1, ITGB3, MUC1, and ACVR1B, or affect JAr-spheroid implantation. However, levonorgestrel and progesterone reduced the ciliary beat frequency in fallopian tubes in a dose-dependent manner. An in vivo model also showed that levonorgestrel and progesterone could lead to embryo retention in the oviducts. Conclusions These findings show that levonorgestrel and progesterone can reduce the ciliary beat frequency without altering receptivity, indicating a possible mechanism for progesterone- or levonorgestrel-induced tubal pregnancy. |
| first_indexed | 2024-12-22T16:02:33Z |
| format | Article |
| id | doaj.art-69e034ffeb3d434aa21ae1b3e7359616 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-22T16:02:33Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-69e034ffeb3d434aa21ae1b3e73596162022-12-21T18:20:40ZengBMCReproductive Biology and Endocrinology1477-78272018-06-011611810.1186/s12958-018-0377-3Effects of Levonorgestrel and progesterone on Oviductal physiology in mammalsCheng Li0Hui-Yu Zhang1Yan Liang2Wei Xia3Qian Zhu4Duo Zhang5Zhen Huang6Gui-Lin Liang7Rui-Hong Xue8Hang Qi9Xiao-Qing He10Jiang-Jing Yuan11Ya-Jing Tan12He-Feng Huang13Jian Zhang14Department of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong UniversityInstitute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong UniversityDepartment of Gynecology, International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityAbstract Background Our previous study indicated that emergency contraception, including levonorgestrel and progesterone, could lead to ectopic pregnancy following contraception failure. However, our understanding of the effects of levonorgestrel and progesterone on oviductal physiology is limited. Methods The receptivity of the fallopian tubal epithelium after levonorgestrel and progesterone treatment was examined through western blots for receptivity markers and JAr-spheroid-fallopian tubal epithelial cell attachment assays. The ciliary beat frequency was analyzed using an inverted bright-field microscope. Furthermore, an in vivo animal model of embryo-tubal transplantation was also studied to determine the effects of levonorgestrel- and progesterone-induced ciliary beat reduction. Results Our results showed that levonorgestrel and progesterone did not change the levels of fallopian tubal epithelial cell receptive markers, including LIF, STAT3, IGFBP1, ITGB3, MUC1, and ACVR1B, or affect JAr-spheroid implantation. However, levonorgestrel and progesterone reduced the ciliary beat frequency in fallopian tubes in a dose-dependent manner. An in vivo model also showed that levonorgestrel and progesterone could lead to embryo retention in the oviducts. Conclusions These findings show that levonorgestrel and progesterone can reduce the ciliary beat frequency without altering receptivity, indicating a possible mechanism for progesterone- or levonorgestrel-induced tubal pregnancy.http://link.springer.com/article/10.1186/s12958-018-0377-3ProgesteroneLevonorgestrelOviductReceptivityCiliary beat frequency |
| spellingShingle | Cheng Li Hui-Yu Zhang Yan Liang Wei Xia Qian Zhu Duo Zhang Zhen Huang Gui-Lin Liang Rui-Hong Xue Hang Qi Xiao-Qing He Jiang-Jing Yuan Ya-Jing Tan He-Feng Huang Jian Zhang Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals Reproductive Biology and Endocrinology Progesterone Levonorgestrel Oviduct Receptivity Ciliary beat frequency |
| title | Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals |
| title_full | Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals |
| title_fullStr | Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals |
| title_full_unstemmed | Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals |
| title_short | Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals |
| title_sort | effects of levonorgestrel and progesterone on oviductal physiology in mammals |
| topic | Progesterone Levonorgestrel Oviduct Receptivity Ciliary beat frequency |
| url | http://link.springer.com/article/10.1186/s12958-018-0377-3 |
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