Detection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposes
Abstract Cholera infection results from the ingestion of water or food contaminated with toxigenic Vibrio cholerae. This study evaluated the occurrence of toxigenic V. cholerae in Asata River, Enugu Metropolis, Nigeria, and estimated V. cholerae infection risks from use of the River water for drinki...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2024-02-01
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| Series: | Discover Water |
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| Online Access: | https://doi.org/10.1007/s43832-024-00059-z |
| _version_ | 1797273317893210112 |
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| author | Chizoba A. Ozochi Christopher C. Okonkwo Emmanuel C. Adukwu Victor C. Ujor Matthew C. Enebe Vincent N. Chigor |
| author_facet | Chizoba A. Ozochi Christopher C. Okonkwo Emmanuel C. Adukwu Victor C. Ujor Matthew C. Enebe Vincent N. Chigor |
| author_sort | Chizoba A. Ozochi |
| collection | DOAJ |
| description | Abstract Cholera infection results from the ingestion of water or food contaminated with toxigenic Vibrio cholerae. This study evaluated the occurrence of toxigenic V. cholerae in Asata River, Enugu Metropolis, Nigeria, and estimated V. cholerae infection risks from use of the River water for drinking, domestic and recreational purposes. Vibrio was detected and quantified using membrane filtration technique and thiosulfate–citrate–bile salts–sucrose agar. Isolates were screened by PCR, using specific primers targeting the internal transcribed spacer (its) region between 16 and 23S rRNA and the cholera toxin (ctx) gene. Sequenced 16SrRNA gene amplicons of two selected isolates were used for phylogenetic analysis. Quantitative microbial risk assessment (QMRA) was conducted using the β-Poisson dose–response model. About 81% (58/72) of Asata River samples recorded Vibrio counts above 1.0 $$\times$$ × 103 cfu/100 mL. Of the fifty Vibrio isolates screened, its was detected in 54% (27/50), out of which 74% (20/27) had the ctx gene of toxigenic V. cholerae. Evolutionary relatedness of the sequenced isolates to V. cholerae was revealed. The estimated risks of cholera infection in persons exposed to untreated Asata River water were above 0.5 for all the exposure scenarios, for both the rainy and dry seasons. The risks were highest (~ 0.9) for exposure via drinking water and annual risk of infection was deduced to have a probability of 1.0. Therefore, dependence on the untreated Asata River water for drinking, recreational, domestic and irrigation purposes may present a potential public health risk of cholera outbreak. We recommend increased monitoring and surveillance of River water for Vibrio abundance and that Asata River be protected from further degradation. |
| first_indexed | 2024-03-07T14:41:42Z |
| format | Article |
| id | doaj.art-69e20c288c914d498b54bbf8ef431288 |
| institution | Directory Open Access Journal |
| issn | 2730-647X |
| language | English |
| last_indexed | 2024-03-07T14:41:42Z |
| publishDate | 2024-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Water |
| spelling | doaj.art-69e20c288c914d498b54bbf8ef4312882024-03-05T20:12:50ZengSpringerDiscover Water2730-647X2024-02-014111610.1007/s43832-024-00059-zDetection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposesChizoba A. Ozochi0Christopher C. Okonkwo1Emmanuel C. Adukwu2Victor C. Ujor3Matthew C. Enebe4Vincent N. Chigor5Water and Public Health Research Group (WPHRG), University of NigeriaBiotechnology Program, Chemistry and Chemical Biology Department, College of Science, The Roux Institute, Northeastern UniversitySchool of Applied Sciences, University of the West of EnglandDepartment of Food Science, University of Wisconsin-MadisonWater and Public Health Research Group (WPHRG), University of NigeriaWater and Public Health Research Group (WPHRG), University of NigeriaAbstract Cholera infection results from the ingestion of water or food contaminated with toxigenic Vibrio cholerae. This study evaluated the occurrence of toxigenic V. cholerae in Asata River, Enugu Metropolis, Nigeria, and estimated V. cholerae infection risks from use of the River water for drinking, domestic and recreational purposes. Vibrio was detected and quantified using membrane filtration technique and thiosulfate–citrate–bile salts–sucrose agar. Isolates were screened by PCR, using specific primers targeting the internal transcribed spacer (its) region between 16 and 23S rRNA and the cholera toxin (ctx) gene. Sequenced 16SrRNA gene amplicons of two selected isolates were used for phylogenetic analysis. Quantitative microbial risk assessment (QMRA) was conducted using the β-Poisson dose–response model. About 81% (58/72) of Asata River samples recorded Vibrio counts above 1.0 $$\times$$ × 103 cfu/100 mL. Of the fifty Vibrio isolates screened, its was detected in 54% (27/50), out of which 74% (20/27) had the ctx gene of toxigenic V. cholerae. Evolutionary relatedness of the sequenced isolates to V. cholerae was revealed. The estimated risks of cholera infection in persons exposed to untreated Asata River water were above 0.5 for all the exposure scenarios, for both the rainy and dry seasons. The risks were highest (~ 0.9) for exposure via drinking water and annual risk of infection was deduced to have a probability of 1.0. Therefore, dependence on the untreated Asata River water for drinking, recreational, domestic and irrigation purposes may present a potential public health risk of cholera outbreak. We recommend increased monitoring and surveillance of River water for Vibrio abundance and that Asata River be protected from further degradation.https://doi.org/10.1007/s43832-024-00059-zCholeraSurface watersDrinking waterToxigenic Vibrio strainsQuantitative microbial risk assessmentHuman health risks |
| spellingShingle | Chizoba A. Ozochi Christopher C. Okonkwo Emmanuel C. Adukwu Victor C. Ujor Matthew C. Enebe Vincent N. Chigor Detection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposes Discover Water Cholera Surface waters Drinking water Toxigenic Vibrio strains Quantitative microbial risk assessment Human health risks |
| title | Detection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposes |
| title_full | Detection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposes |
| title_fullStr | Detection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposes |
| title_full_unstemmed | Detection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposes |
| title_short | Detection and quantitative microbial risk assessment of pathogenic Vibrio cholerae in a river used for drinking, domestic, fresh produce irrigation and recreational purposes |
| title_sort | detection and quantitative microbial risk assessment of pathogenic vibrio cholerae in a river used for drinking domestic fresh produce irrigation and recreational purposes |
| topic | Cholera Surface waters Drinking water Toxigenic Vibrio strains Quantitative microbial risk assessment Human health risks |
| url | https://doi.org/10.1007/s43832-024-00059-z |
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